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Article: Expression of leptin and leptin receptors in gestational trophoblastic diseases

TitleExpression of leptin and leptin receptors in gestational trophoblastic diseases
Authors
KeywordsChoriocarcinoma
Hydatidiform mole
Leptin
Leptin receptor
Placental site trophoblastic tumor
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2004, v. 95 n. 2, p. 299-306 How to Cite?
AbstractTo investigate the expression profile of leptin and leptin receptors in gestational trophoblastic diseases (GTDs). Using immunohistochemical staining on archival paraffin-embedded tissue sections, we studied the expression of leptin and leptin receptor in hydatidiform moles, with gestational age-matched normal first-trimester placenta used as control. A total of 38 cases of hydatidiform moles were studied, including 20 complete moles (CHMs) and 18 partial moles (PHMs). Among them, 10 cases of the CHM group and 8 cases of the PHM group subsequently developed residual trophoblastic disease (RTD). In addition, two cases of choriocarcinoma and three cases of placental site trophoblastic tumor (PSTT) were also studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) was further performed using RNA extracted from frozen tissue (five CHMs, four PHMs and nine normal first-trimester placenta) to study the expression of leptin and individual leptin receptor isoforms at the transcription level. In all tissue sections, immunostaining signal was shown in the cytoplasmic compartment of cytotrophoblasts and syncytiotrophoblasts, with much stronger staining in the former. Significantly higher immunostaining intensity was shown for both leptin (P < 0.05) and leptin receptor (P < 0.001) in both CHMs and PHMs compared to normal first-trimester placenta. There was no significant difference between those cases subsequently developing RTD and those which did not (P > 0.05). In the choriocarcinoma and PSTT cases, intense immunostaining was found in the tumor cells. RT-PCR revealed that the expression of leptin and all leptin receptor isoforms were significantly higher in both CHMs and PHMs than in normal placenta (P < 0.05). There is up-regulated expression of leptin and leptin receptor in GTDs. However, there is no obvious correlation with the development of RTD. The exact role played by leptin and its receptors in the pathogenesis of GTDs awaits further investigations. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88466
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, RHWen_HK
dc.contributor.authorYu, MMYen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorWong, YFen_HK
dc.date.accessioned2010-09-06T09:43:45Z-
dc.date.available2010-09-06T09:43:45Z-
dc.date.issued2004en_HK
dc.identifier.citationGynecologic Oncology, 2004, v. 95 n. 2, p. 299-306en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88466-
dc.description.abstractTo investigate the expression profile of leptin and leptin receptors in gestational trophoblastic diseases (GTDs). Using immunohistochemical staining on archival paraffin-embedded tissue sections, we studied the expression of leptin and leptin receptor in hydatidiform moles, with gestational age-matched normal first-trimester placenta used as control. A total of 38 cases of hydatidiform moles were studied, including 20 complete moles (CHMs) and 18 partial moles (PHMs). Among them, 10 cases of the CHM group and 8 cases of the PHM group subsequently developed residual trophoblastic disease (RTD). In addition, two cases of choriocarcinoma and three cases of placental site trophoblastic tumor (PSTT) were also studied. Reverse transcriptase-polymerase chain reaction (RT-PCR) was further performed using RNA extracted from frozen tissue (five CHMs, four PHMs and nine normal first-trimester placenta) to study the expression of leptin and individual leptin receptor isoforms at the transcription level. In all tissue sections, immunostaining signal was shown in the cytoplasmic compartment of cytotrophoblasts and syncytiotrophoblasts, with much stronger staining in the former. Significantly higher immunostaining intensity was shown for both leptin (P < 0.05) and leptin receptor (P < 0.001) in both CHMs and PHMs compared to normal first-trimester placenta. There was no significant difference between those cases subsequently developing RTD and those which did not (P > 0.05). In the choriocarcinoma and PSTT cases, intense immunostaining was found in the tumor cells. RT-PCR revealed that the expression of leptin and all leptin receptor isoforms were significantly higher in both CHMs and PHMs than in normal placenta (P < 0.05). There is up-regulated expression of leptin and leptin receptor in GTDs. However, there is no obvious correlation with the development of RTD. The exact role played by leptin and its receptors in the pathogenesis of GTDs awaits further investigations. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subjectChoriocarcinomaen_HK
dc.subjectHydatidiform moleen_HK
dc.subjectLeptinen_HK
dc.subjectLeptin receptoren_HK
dc.subjectPlacental site trophoblastic tumoren_HK
dc.subject.meshChoriocarcinoma - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydatidiform Mole - metabolismen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLeptin - biosynthesisen_HK
dc.subject.meshPlacenta - metabolismen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshProtein Isoformsen_HK
dc.subject.meshReceptors, Cell Surface - biosynthesisen_HK
dc.subject.meshReceptors, Leptinen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshUp-Regulationen_HK
dc.subject.meshUterine Neoplasms - metabolismen_HK
dc.titleExpression of leptin and leptin receptors in gestational trophoblastic diseasesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=95&issue=2&spage=299&epage=306&date=2004&atitle=Expression+of+leptin+and+leptin+receptors+in+gestational+trophoblastic+diseasesen_HK
dc.identifier.emailLi, RHW: raymondli@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authorityLi, RHW=rp01649en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygyno.2004.06.040en_HK
dc.identifier.pmid15491749-
dc.identifier.scopuseid_2-s2.0-7444270348en_HK
dc.identifier.hkuros104928en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-7444270348&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue2en_HK
dc.identifier.spage299en_HK
dc.identifier.epage306en_HK
dc.identifier.isiWOS:000224937000004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, RHW=7404724295en_HK
dc.identifier.scopusauthoridYu, MMY=8581464300en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridWong, YF=7403041448en_HK

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