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Article: The LMP1 gene isolated from Russian nasopharyngeal carcinoma has no 30-bp deletion

TitleThe LMP1 gene isolated from Russian nasopharyngeal carcinoma has no 30-bp deletion
Authors
Issue Date2001
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2001, v. 91 n. 6, p. 815-821 How to Cite?
AbstractThe Epstein-Barr virus (EBV) is tightly linked to the induction of undifferentiated nasopharyngeal carcinoma (NPC), a tumour endemic in certain areas of southeast Asia. The LMP1 gene encoded by EBV is a classical oncogene due to its ability to transform rodent fibroblasts. LMP1 is absolutely essential for transformation of B cells by the virus and is one of the few EBV genes found to be expressed in NPC. It was originally shown that the LMP1 gene from NPC harbours a deletion of 30 bp in the 3′ part of the gene. However, this deletion is also present in the virus spread in healthy people of the areas endemic for NPC and also in other EBV-positive tumours as well as in healthy carriers. We isolated and sequenced the LMP1 gene obtained from tissue of 7 Russian patients with NPC and 1 German patient with an NPC-like tumour of the parotid gland (PG) and compared them with the LMP1 gene isolated from peripheral blood lymphocytes (PBLs) of 6 Russian and 4 German healthy EBV-positive carriers. Neither the Russian NPC cases nor the German NPC-like tumour harboured an LMP1 gene with the 30-bp deletion, while 1 Russian and 2 German carriers contained the LMP1 gene with the 30-bp deletion. In addition, the LMP1 gene isolated from PBLs of the German patient was virtually identical to the gene isolated from the primary tumour. Functional analysis showed no correlation between the presence or absence of the 30-bp deletion and the level of induction of the transcription factors NFκB and jun/AP-1 caused by LMP1. These data indicate that the 30-bp deletion is not a factor predisposing for NPC. Comparison of the DNA sequences revealed that the LMP1 genes present in the NPCs most likely represent the strain persisting in the general population. © 2001 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/88425
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHahn, Pen_HK
dc.contributor.authorNovikova, Een_HK
dc.contributor.authorScherback, Len_HK
dc.contributor.authorJanik, Cen_HK
dc.contributor.authorPavlish, Oen_HK
dc.contributor.authorArkhipov, Ven_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorMüllerLantzsch, Nen_HK
dc.contributor.authorGurtsevitch, Ven_HK
dc.contributor.authorGrässer, FAen_HK
dc.date.accessioned2010-09-06T09:43:12Z-
dc.date.available2010-09-06T09:43:12Z-
dc.date.issued2001en_HK
dc.identifier.citationInternational Journal Of Cancer, 2001, v. 91 n. 6, p. 815-821en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88425-
dc.description.abstractThe Epstein-Barr virus (EBV) is tightly linked to the induction of undifferentiated nasopharyngeal carcinoma (NPC), a tumour endemic in certain areas of southeast Asia. The LMP1 gene encoded by EBV is a classical oncogene due to its ability to transform rodent fibroblasts. LMP1 is absolutely essential for transformation of B cells by the virus and is one of the few EBV genes found to be expressed in NPC. It was originally shown that the LMP1 gene from NPC harbours a deletion of 30 bp in the 3′ part of the gene. However, this deletion is also present in the virus spread in healthy people of the areas endemic for NPC and also in other EBV-positive tumours as well as in healthy carriers. We isolated and sequenced the LMP1 gene obtained from tissue of 7 Russian patients with NPC and 1 German patient with an NPC-like tumour of the parotid gland (PG) and compared them with the LMP1 gene isolated from peripheral blood lymphocytes (PBLs) of 6 Russian and 4 German healthy EBV-positive carriers. Neither the Russian NPC cases nor the German NPC-like tumour harboured an LMP1 gene with the 30-bp deletion, while 1 Russian and 2 German carriers contained the LMP1 gene with the 30-bp deletion. In addition, the LMP1 gene isolated from PBLs of the German patient was virtually identical to the gene isolated from the primary tumour. Functional analysis showed no correlation between the presence or absence of the 30-bp deletion and the level of induction of the transcription factors NFκB and jun/AP-1 caused by LMP1. These data indicate that the 30-bp deletion is not a factor predisposing for NPC. Comparison of the DNA sequences revealed that the LMP1 genes present in the NPCs most likely represent the strain persisting in the general population. © 2001 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAlkaline Phosphatase - metabolismen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshDNA Primers - chemistryen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Deletionen_HK
dc.subject.meshGenetic Variationen_HK
dc.subject.meshGermany - epidemiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLuciferases - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNF-kappa B - metabolismen_HK
dc.subject.meshNasopharyngeal Neoplasms - chemistry - ethnology - virologyen_HK
dc.subject.meshParotid Neoplasms - chemistry - ethnology - virologyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshRussia - epidemiologyen_HK
dc.subject.meshSequence Homology, Nucleic Aciden_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.subject.meshViral Matrix Proteins - genetics - isolation & purificationen_HK
dc.titleThe LMP1 gene isolated from Russian nasopharyngeal carcinoma has no 30-bp deletionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=91&issue=6&spage=815&epage=821&date=2001&atitle=The+LMP1+gene+isolated+from+Russian+nasopharyngeal+carcinoma+has+no+30-bp+deletionen_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1097-0215(200002)9999:9999<::AID-IJC1122>3.0.CO;2-Wen_HK
dc.identifier.pmid11275985-
dc.identifier.scopuseid_2-s2.0-0035869653en_HK
dc.identifier.hkuros64218en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035869653&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume91en_HK
dc.identifier.issue6en_HK
dc.identifier.spage815en_HK
dc.identifier.epage821en_HK
dc.identifier.isiWOS:000167073300011-
dc.publisher.placeUnited Statesen_HK

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