File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1006/bbrc.2000.3614
- Scopus: eid_2-s2.0-0034609949
- PMID: 11027611
- WOS: WOS:000089794300060
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Molecular cloning and functional analysis of the promoter region of rat nonmuscle myosin heavy chain-B gene
Title | Molecular cloning and functional analysis of the promoter region of rat nonmuscle myosin heavy chain-B gene |
---|---|
Authors | |
Keywords | Alkaline phosphatase reporter assay Nonmuscle myosin heavy chain-B Promoter Rat 6 fibroblast Transcriptional factor Transformation |
Issue Date | 2000 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 2000, v. 276 n. 3, p. 1203-1209 How to Cite? |
Abstract | Rat nonmuscle myosin heavy chain-B (r-nmMHC-B) mRNA was previously found downregulated in Rat 6 fibroblasts transformed by mutant p53(val135) [J. W. P. Yam, J. Y. Zheng, and W. L. W. Hsiao (1987) Biochem. Biophys. Res. Commun. 266, 472-480]. Overexpression of exogenous r-nmMHC-B could partially reverse the transforming phenotypes both in vitro and in vivo. The downregulation of r-nmMHC-B was also observed in Rat 6 transformed by c-H-ras and v-myc oncogenes. We cloned a 5.2-kb r-nmMHC-B promoter region. Sequence analysis of -1248 to +1 revealed no TATA box, but did show that it contained CAAT boxes, E12/E47, MyoD, MEF, E2F, CREB, and SP1 binding sites. Based on transient reporter assays, the promoter/enhancer activities were unusually extended to the entire 5.2 kb region in normal Rat 6 cultures, but markedly suppressed in p53(val135)-, and c-H-ras-transformed cells. The activity detected by the reporter assay corresponded to levels of mRNA as analyzed previously by Northern blots in each respective cell line. Thus, the switch-off of the r-nmMHC-B in the transformed cells is very likely controlled by upstream transcriptional factors, which might have been altered in the course of neo-plastic transformation. (C) 2000 Academic Press. |
Persistent Identifier | http://hdl.handle.net/10722/88340 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yam, JWP | en_HK |
dc.contributor.author | Chan, KW | en_HK |
dc.contributor.author | Li, N | en_HK |
dc.contributor.author | Hsiao, WLWendy | en_HK |
dc.date.accessioned | 2010-09-06T09:42:06Z | - |
dc.date.available | 2010-09-06T09:42:06Z | - |
dc.date.issued | 2000 | en_HK |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 2000, v. 276 n. 3, p. 1203-1209 | en_HK |
dc.identifier.issn | 0006-291X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/88340 | - |
dc.description.abstract | Rat nonmuscle myosin heavy chain-B (r-nmMHC-B) mRNA was previously found downregulated in Rat 6 fibroblasts transformed by mutant p53(val135) [J. W. P. Yam, J. Y. Zheng, and W. L. W. Hsiao (1987) Biochem. Biophys. Res. Commun. 266, 472-480]. Overexpression of exogenous r-nmMHC-B could partially reverse the transforming phenotypes both in vitro and in vivo. The downregulation of r-nmMHC-B was also observed in Rat 6 transformed by c-H-ras and v-myc oncogenes. We cloned a 5.2-kb r-nmMHC-B promoter region. Sequence analysis of -1248 to +1 revealed no TATA box, but did show that it contained CAAT boxes, E12/E47, MyoD, MEF, E2F, CREB, and SP1 binding sites. Based on transient reporter assays, the promoter/enhancer activities were unusually extended to the entire 5.2 kb region in normal Rat 6 cultures, but markedly suppressed in p53(val135)-, and c-H-ras-transformed cells. The activity detected by the reporter assay corresponded to levels of mRNA as analyzed previously by Northern blots in each respective cell line. Thus, the switch-off of the r-nmMHC-B in the transformed cells is very likely controlled by upstream transcriptional factors, which might have been altered in the course of neo-plastic transformation. (C) 2000 Academic Press. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_HK |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_HK |
dc.subject | Alkaline phosphatase reporter assay | - |
dc.subject | Nonmuscle myosin heavy chain-B | - |
dc.subject | Promoter | - |
dc.subject | Rat 6 fibroblast | - |
dc.subject | Transcriptional factor | - |
dc.subject | Transformation | - |
dc.subject.mesh | Animals | en_HK |
dc.subject.mesh | Base Sequence | en_HK |
dc.subject.mesh | Binding Sites | en_HK |
dc.subject.mesh | Cell Line, Transformed | en_HK |
dc.subject.mesh | Cell Transformation, Neoplastic - genetics | en_HK |
dc.subject.mesh | Cloning, Molecular | en_HK |
dc.subject.mesh | Conserved Sequence - genetics | en_HK |
dc.subject.mesh | Fibroblasts - metabolism | en_HK |
dc.subject.mesh | Gene Expression Regulation | en_HK |
dc.subject.mesh | Genes, Reporter - genetics | en_HK |
dc.subject.mesh | Genes, myc - genetics | en_HK |
dc.subject.mesh | Genes, p53 - genetics | en_HK |
dc.subject.mesh | Genes, ras - genetics | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Molecular Sequence Data | en_HK |
dc.subject.mesh | Mutation - genetics | en_HK |
dc.subject.mesh | Myosin Heavy Chains - genetics | en_HK |
dc.subject.mesh | Nonmuscle Myosin Type IIB | en_HK |
dc.subject.mesh | Promoter Regions, Genetic - genetics | en_HK |
dc.subject.mesh | RNA, Messenger - analysis - genetics | en_HK |
dc.subject.mesh | Rats | en_HK |
dc.subject.mesh | Response Elements - genetics | en_HK |
dc.subject.mesh | Sequence Alignment | en_HK |
dc.subject.mesh | Transcription, Genetic - genetics | en_HK |
dc.subject.mesh | Tumor Suppressor Protein p53 - genetics - metabolism | en_HK |
dc.title | Molecular cloning and functional analysis of the promoter region of rat nonmuscle myosin heavy chain-B gene | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=276&spage=1203&epage=1209&date=2000&atitle=Molecular+cloning+and+functional+analysis+of+the+promoter+region+of+rat+nonmuscle+myosin+heavy+chain-B+gene | en_HK |
dc.identifier.email | Yam, JWP:judyyam@pathology.hku.hk | en_HK |
dc.identifier.authority | Yam, JWP=rp00468 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1006/bbrc.2000.3614 | en_HK |
dc.identifier.pmid | 11027611 | - |
dc.identifier.scopus | eid_2-s2.0-0034609949 | en_HK |
dc.identifier.hkuros | 56449 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034609949&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 276 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 1203 | en_HK |
dc.identifier.epage | 1209 | en_HK |
dc.identifier.isi | WOS:000089794300060 | - |
dc.publisher.place | United States | en_HK |
dc.identifier.issnl | 0006-291X | - |