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Article: Establishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinoma

TitleEstablishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinoma
Authors
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
Citation
Cancer Genetics And Cytogenetics, 2000, v. 118 n. 2, p. 112-120 How to Cite?
AbstractThe establishment of an esophageal cancer cell line can facilitate the search for molecular mechanisms involved in esophageal carcinogenesis. A new human cancer cell line, HKESC-1, was established from a primary moderately-differentiated squamous cell carcinoma of the esophagus from a 47-year-old Hong Kong Chinese man. The pathological characteristics (morphology, immunohistochemical, and electron microscopic studies), the tumorigenecity in nude mice, the cytogenetic features, the DNA ploidy, and telomerase activity of the cell line were investigated. The HKESC-1 cells have been maintained continuously in vitro for more than 16 months and passaged over 96 times. HKESC-1 cells grow as a monolayer, with a doubling time of 46 hours. The HKESC-1 cells are of a squamous epithelial origin, as shown by their immunopositivity with the anti-cytokeratin antibodies and ultrastructural demonstration of tonofilaments and desmosomes. The HKESC-1 cells possess characteristics of malignancy because they are highly tumorigenic in nude mice and have strong telomerase activity. The HKESC-1 cells had an aneuploid DNA content, as demonstrated by flow cytometric analysis. Cytogenetic analysis revealed hyperdiploidy of greater than 50 in 80% of analyzable metaphases. Chromosome gains and losses were common, and loss of the Y chromosome was a consistent numerical aberration. Additionally, many structural chromosomal abnormalities were encountered, with frequent breakpoints at 1p32, 7p22, 7q34, and 20q13. This newly established cell line serves as a useful model for studying the molecular pathogenesis, and testing new therapeutic reagents for esophageal squamous cell carcinoma. Copyright (C) 2000 Elsevier Science Inc.
Persistent Identifierhttp://hdl.handle.net/10722/88321
ISSN
2012 Impact Factor: 1.929
2013 SCImago Journal Rankings: 0.872
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, YCen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorFang, WGen_HK
dc.contributor.authorMa, ESKen_HK
dc.contributor.authorChan, LCen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T09:41:50Z-
dc.date.available2010-09-06T09:41:50Z-
dc.date.issued2000en_HK
dc.identifier.citationCancer Genetics And Cytogenetics, 2000, v. 118 n. 2, p. 112-120en_HK
dc.identifier.issn0165-4608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88321-
dc.description.abstractThe establishment of an esophageal cancer cell line can facilitate the search for molecular mechanisms involved in esophageal carcinogenesis. A new human cancer cell line, HKESC-1, was established from a primary moderately-differentiated squamous cell carcinoma of the esophagus from a 47-year-old Hong Kong Chinese man. The pathological characteristics (morphology, immunohistochemical, and electron microscopic studies), the tumorigenecity in nude mice, the cytogenetic features, the DNA ploidy, and telomerase activity of the cell line were investigated. The HKESC-1 cells have been maintained continuously in vitro for more than 16 months and passaged over 96 times. HKESC-1 cells grow as a monolayer, with a doubling time of 46 hours. The HKESC-1 cells are of a squamous epithelial origin, as shown by their immunopositivity with the anti-cytokeratin antibodies and ultrastructural demonstration of tonofilaments and desmosomes. The HKESC-1 cells possess characteristics of malignancy because they are highly tumorigenic in nude mice and have strong telomerase activity. The HKESC-1 cells had an aneuploid DNA content, as demonstrated by flow cytometric analysis. Cytogenetic analysis revealed hyperdiploidy of greater than 50 in 80% of analyzable metaphases. Chromosome gains and losses were common, and loss of the Y chromosome was a consistent numerical aberration. Additionally, many structural chromosomal abnormalities were encountered, with frequent breakpoints at 1p32, 7p22, 7q34, and 20q13. This newly established cell line serves as a useful model for studying the molecular pathogenesis, and testing new therapeutic reagents for esophageal squamous cell carcinoma. Copyright (C) 2000 Elsevier Science Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergeneen_HK
dc.relation.ispartofCancer Genetics and Cytogeneticsen_HK
dc.rightsCancer Genetics and Cytogenetics. Copyright © Elsevier Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarcinoma, Squamous Cell - genetics - pathologyen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshDNA, Neoplasm - analysisen_HK
dc.subject.meshEsophageal Neoplasms - genetics - pathologyen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNeoplasm Transplantationen_HK
dc.subject.meshTelomerase - analysisen_HK
dc.subject.meshTransplantation, Heterologousen_HK
dc.titleEstablishment and characterization of HKESC-1, a new cancer cell line from human esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0165-4608&volume=118&issue=2&spage=112&epage=120&date=2000&atitle=Establishment+and+characterization+of+HKESC-1,+a+new+cancer+cell+line+from+human+esophageal+squamous+cell+carcinomaen_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0165-4608(99)00193-4en_HK
dc.identifier.pmid10748291-
dc.identifier.scopuseid_2-s2.0-0034656223en_HK
dc.identifier.hkuros50823en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034656223&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume118en_HK
dc.identifier.issue2en_HK
dc.identifier.spage112en_HK
dc.identifier.epage120en_HK
dc.identifier.isiWOS:000086462800004-
dc.publisher.placeUnited Statesen_HK

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