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Article: A study of gut immunity to enteral endotoxin in rats of different ages: A possible cause for necrotizing enterocolitis

TitleA study of gut immunity to enteral endotoxin in rats of different ages: A possible cause for necrotizing enterocolitis
Authors
Issue Date2002
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurg
Citation
Journal Of Pediatric Surgery, 2002, v. 37 n. 10, p. 1435-1440 How to Cite?
AbstractPurpose: Immature gut immunity can be a predisposing factor for necrotizing enterocolitis (NEC). The gut active immunity and innate defense to the Escherichia coli endotoxin lipopolysaccharide (LPS) in immature and mature rats were studied. Methods: LPS, started at a dose of 10 mg/kg, was instilled into the stomachs of fetal, newborn, 1-month and 3-monthold rats. Boost doses and normal saline control instillations were given on day 14. Rats that died after instillation had detailed postmortem examinations. For survivors, a group of 6 immunized and 6 controls were killed on day 7 for the collection of serum, spleens, mesenteric lymph nodes, and small intestines. Lymphocytes (106) prepared from each tissue sample of individual group were cultured for 5 days. Serum and supernatant were analyzed for IgA and anti-E coli IgA levels. Results: All control rats survived. The doses of LPS given were 10, 5, 2.5, and 1.25 mg/kg. All fetal rats died after LPS instillation. Half-lethal dose for newborns was 2.5 mg/kg. One-month and 3-month-old rats survived all doses of LPS. The cause of death was endotoxemia. The serum IgA and total supernatant anti- E coli IgA levels of rats of all ages studied showed no significant difference. Conclusion: The poor innate gut defense, not so much the active immunity, may provide an explanation for the susceptibility of the premature babies and newborn infants to the development of NEC. Copyright 2002, Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88286
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.802
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KLen_HK
dc.contributor.authorHo, JCYen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T09:41:22Z-
dc.date.available2010-09-06T09:41:22Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Pediatric Surgery, 2002, v. 37 n. 10, p. 1435-1440en_HK
dc.identifier.issn0022-3468en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88286-
dc.description.abstractPurpose: Immature gut immunity can be a predisposing factor for necrotizing enterocolitis (NEC). The gut active immunity and innate defense to the Escherichia coli endotoxin lipopolysaccharide (LPS) in immature and mature rats were studied. Methods: LPS, started at a dose of 10 mg/kg, was instilled into the stomachs of fetal, newborn, 1-month and 3-monthold rats. Boost doses and normal saline control instillations were given on day 14. Rats that died after instillation had detailed postmortem examinations. For survivors, a group of 6 immunized and 6 controls were killed on day 7 for the collection of serum, spleens, mesenteric lymph nodes, and small intestines. Lymphocytes (106) prepared from each tissue sample of individual group were cultured for 5 days. Serum and supernatant were analyzed for IgA and anti-E coli IgA levels. Results: All control rats survived. The doses of LPS given were 10, 5, 2.5, and 1.25 mg/kg. All fetal rats died after LPS instillation. Half-lethal dose for newborns was 2.5 mg/kg. One-month and 3-month-old rats survived all doses of LPS. The cause of death was endotoxemia. The serum IgA and total supernatant anti- E coli IgA levels of rats of all ages studied showed no significant difference. Conclusion: The poor innate gut defense, not so much the active immunity, may provide an explanation for the susceptibility of the premature babies and newborn infants to the development of NEC. Copyright 2002, Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/jpedsurgen_HK
dc.relation.ispartofJournal of Pediatric Surgeryen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Newborn - growth & development - immunologyen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshEndotoxemia - immunology - pathologyen_HK
dc.subject.meshEndotoxins - administration & dosage - immunologyen_HK
dc.subject.meshEnterocolitis, Necrotizing - etiology - immunology - pathologyen_HK
dc.subject.meshEscherichia coli - immunologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFetus - immunologyen_HK
dc.subject.meshImmunity, Innate - physiologyen_HK
dc.subject.meshIntestines - immunology - pathologyen_HK
dc.subject.meshLipopolysaccharides - immunologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.titleA study of gut immunity to enteral endotoxin in rats of different ages: A possible cause for necrotizing enterocolitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3468&volume=37 &issue=10&spage=1435&epage=1440&date=2002&atitle=A+study+of+gut+immunity+to+enteral+endotoxin+in+rats+of+different+ages:+a+possible+cause+for+necrotizing+enterocolitisen_HK
dc.identifier.emailChan, KW:hrmtckw@hku.hken_HK
dc.identifier.emailTam, PKH:paultam@hkucc.hku.hken_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1053/jpsu.2002.35407en_HK
dc.identifier.pmid12378449en_HK
dc.identifier.scopuseid_2-s2.0-0036788995en_HK
dc.identifier.hkuros77597en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036788995&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1435en_HK
dc.identifier.epage1440en_HK
dc.identifier.isiWOS:000178353600010-
dc.publisher.placeUnited Statesen_HK

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