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Article: Transcriptional regulation of BMP-4 in the Xenopus embryo: Analysis of genomic BMP-4 and its promoter

TitleTranscriptional regulation of BMP-4 in the Xenopus embryo: Analysis of genomic BMP-4 and its promoter
Authors
Issue Date1998
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1998, v. 250 n. 2, p. 516-530 How to Cite?
AbstractRecent experiments in the Xenopus embryo suggest that proper regulation of BMP-4 signaling is critical to the dorsal ventral specification of both mesoderm and ectoderm. Regulation of BMP-4 signaling is known to occur extracellularly by direct binding with chordin, noggin, and follistatin, and intracellularly through the antagonistic signal interaction with dorsalizing TGF-β family member activin. However, the tight repressional regulation of BMP transcription may also be required to sustain the dorsal and neural status of the induced cells. Here we demonstrate that the dominant negative mutant of the BMP receptor (DN-BR) or the BMP-4 antagonizers, chordin and noggin, negatively regulate BMP-4 transcription in animal cap explants. We suggest that repression of BMP-4 transcription is important in the maintenance of dorsal fate and that continuous input of BMP-4 signaling is required to sustain the expression of BMP-4 transcription in the maintenance of epidermal/ventral fate. Consistent with this postulation, we found that the promoter region of the isolated BMP-4 genomic DNA includes several consensus binding sites for transcriptional regulators functioning under BMP-4 signaling such as GATA binding and ventralizing homeobox genes. In a functional assay we found that the GATA binding and ventral homeobox proteins can positively modulate BMP-4 promoter activity. We also observed that DN-BR decreases BMP-4 promoter activity. This was likely due to a repression of the above-mentioned transcription factors. The significance of these observations to embryonic patterning is discussed.
Persistent Identifierhttp://hdl.handle.net/10722/88043
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKim, Jen_HK
dc.contributor.authorAult, KTen_HK
dc.contributor.authorChen, HDen_HK
dc.contributor.authorXu, RHen_HK
dc.contributor.authorRoh, DHen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorPark, MJen_HK
dc.contributor.authorKung, HFen_HK
dc.date.accessioned2010-09-06T09:37:54Z-
dc.date.available2010-09-06T09:37:54Z-
dc.date.issued1998en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 1998, v. 250 n. 2, p. 516-530en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/88043-
dc.description.abstractRecent experiments in the Xenopus embryo suggest that proper regulation of BMP-4 signaling is critical to the dorsal ventral specification of both mesoderm and ectoderm. Regulation of BMP-4 signaling is known to occur extracellularly by direct binding with chordin, noggin, and follistatin, and intracellularly through the antagonistic signal interaction with dorsalizing TGF-β family member activin. However, the tight repressional regulation of BMP transcription may also be required to sustain the dorsal and neural status of the induced cells. Here we demonstrate that the dominant negative mutant of the BMP receptor (DN-BR) or the BMP-4 antagonizers, chordin and noggin, negatively regulate BMP-4 transcription in animal cap explants. We suggest that repression of BMP-4 transcription is important in the maintenance of dorsal fate and that continuous input of BMP-4 signaling is required to sustain the expression of BMP-4 transcription in the maintenance of epidermal/ventral fate. Consistent with this postulation, we found that the promoter region of the isolated BMP-4 genomic DNA includes several consensus binding sites for transcriptional regulators functioning under BMP-4 signaling such as GATA binding and ventralizing homeobox genes. In a functional assay we found that the GATA binding and ventral homeobox proteins can positively modulate BMP-4 promoter activity. We also observed that DN-BR decreases BMP-4 promoter activity. This was likely due to a repression of the above-mentioned transcription factors. The significance of these observations to embryonic patterning is discussed.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.titleTranscriptional regulation of BMP-4 in the Xenopus embryo: Analysis of genomic BMP-4 and its promoteren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=250&spage=516&epage=530&date=1998&atitle=Transcriptional+regulation+of+BMP-4+in+the++Xenopus+embryo:+analysis+of+genomic+BMP-4+and+its+promoteren_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/bbrc.1998.9280en_HK
dc.identifier.pmid9753664-
dc.identifier.scopuseid_2-s2.0-0344301936en_HK
dc.identifier.hkuros46064en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344301936&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume250en_HK
dc.identifier.issue2en_HK
dc.identifier.spage516en_HK
dc.identifier.epage530en_HK
dc.identifier.isiWOS:000076088000060-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKim, J=7601362280en_HK
dc.identifier.scopusauthoridAult, KT=7005241219en_HK
dc.identifier.scopusauthoridChen, HD=7501625999en_HK
dc.identifier.scopusauthoridXu, RH=35243812400en_HK
dc.identifier.scopusauthoridRoh, DH=7005887108en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridPark, MJ=7404491223en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK

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