File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Novel noninvasive approach to study the pathogenesis of chronic diabetic neuropathy

TitleNovel noninvasive approach to study the pathogenesis of chronic diabetic neuropathy
Authors
Keywordsdiabetic neuropathy
epidermal nerve fibre density
pain
novelapproach
YFP
Issue Date2004
PublisherBlackwell Publishing Ltd.
Citation
The 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 59 Abstract no. P22-23 How to Cite?
AbstractDiabetic neuropathy is characterized by hyperalgesia, tactile allodynia andloss of sensation. Reduction in small fibres in the skin is observed in chronicdiabetic patients. In rats, short-term exposure to diabetes did not affect theepidermal nerve fibre density (ENFD) but increase in ENFD was observedafter longer exposure to diabetes, which is different from diabetic neuropathypatients. These discrepancy may rise from the invasive methods, whichinvolves many steps like skin biopsy and PGP9.5 staining. Here we made useof YFP transgenic mice that express high level of yellowish-green fluorescentprotein in motor and sensory neuronal cell bodies, axons and the terminals.They were induced to become diabetic by streptozotocin injection (200 mg/kg bw). In the anesthetized mice, the YFP positive epidermal nerve fibreswere quantitated under fluorescent stereo-microscope at different time pointof diabetes. There was no change in ENFD at 4 weeks and 8 weeks ofdiabetes, but there was reduction of ENFD at 22nd weeks. The delayed heatsensation response was observed in diabetic YFP mice at 4th, 8th, 22nd weekof diabetes. The present data showing no ENFD change at early stages ofdiabetes and reduction of ENFD at late stage of diabetes is consistent to theprevious report from the diabetic patients. This is the first novel noninvasivemethod to document the loss of epidermal nerve fibres and loss of sensationin chronic diabetic animal models. The YFP transgenic mouse model is aninvaluable tool for investigating the mechanism of the aetiology of diabeticneuropathy and determining therapeutic efficacy of experimental drugs inchronic diabetic animals.
Persistent Identifierhttp://hdl.handle.net/10722/88041
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorChen, AYSen_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T09:37:52Z-
dc.date.available2010-09-06T09:37:52Z-
dc.date.issued2004en_HK
dc.identifier.citationThe 6th Biennial Meeting of the Asian-Pacific Society for Neurochemistry, Hong Kong, 4-7 February 2004. In Journal of Neurochemistry, 2004, v. 88 n. S1, p. 59 Abstract no. P22-23en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88041-
dc.description.abstractDiabetic neuropathy is characterized by hyperalgesia, tactile allodynia andloss of sensation. Reduction in small fibres in the skin is observed in chronicdiabetic patients. In rats, short-term exposure to diabetes did not affect theepidermal nerve fibre density (ENFD) but increase in ENFD was observedafter longer exposure to diabetes, which is different from diabetic neuropathypatients. These discrepancy may rise from the invasive methods, whichinvolves many steps like skin biopsy and PGP9.5 staining. Here we made useof YFP transgenic mice that express high level of yellowish-green fluorescentprotein in motor and sensory neuronal cell bodies, axons and the terminals.They were induced to become diabetic by streptozotocin injection (200 mg/kg bw). In the anesthetized mice, the YFP positive epidermal nerve fibreswere quantitated under fluorescent stereo-microscope at different time pointof diabetes. There was no change in ENFD at 4 weeks and 8 weeks ofdiabetes, but there was reduction of ENFD at 22nd weeks. The delayed heatsensation response was observed in diabetic YFP mice at 4th, 8th, 22nd weekof diabetes. The present data showing no ENFD change at early stages ofdiabetes and reduction of ENFD at late stage of diabetes is consistent to theprevious report from the diabetic patients. This is the first novel noninvasivemethod to document the loss of epidermal nerve fibres and loss of sensationin chronic diabetic animal models. The YFP transgenic mouse model is aninvaluable tool for investigating the mechanism of the aetiology of diabeticneuropathy and determining therapeutic efficacy of experimental drugs inchronic diabetic animals.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectdiabetic neuropathy-
dc.subjectepidermal nerve fibre density-
dc.subjectpain-
dc.subjectnovelapproach-
dc.subjectYFP-
dc.titleNovel noninvasive approach to study the pathogenesis of chronic diabetic neuropathyen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=88 &issue=Suppl 1&spage=59&epage=&date=2004&atitle=Novel+non-invasive+approach+to+study+the+pathogenesis+of+chronic+diabetic+neuropathyen_HK
dc.identifier.emailChen, AYS: ayschen@hkucc.hku.hken_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1474-1644.2003.2314p22_01.x-
dc.identifier.hkuros91234en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats