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Conference Paper: Over-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia

TitleOver-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia
Authors
KeywordsAstrocytes
Cytokines
Endothelin
Ischemia
Transgenic mouse
Issue Date2003
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159
Citation
Journal of Neurochemistry, 2003, v. 87 suppl. s1, p. 150, abstract no. P14-27 How to Cite?
AbstractPreviously, we reported that the expression of endothelin (ET-1) is induced in astrocytes and endothelial cells after cerebral hypoxia/ischemia. Recently, we also showed that transgenic mice over-expressing ET-1 in astrocytes displayed more severe neurologic deficits and increased brain infract following transient middle cerebral artery occlusion (MCAO for 2 h followed by 22 h reperfusion) compared with those of non-transgenic mice. However, the underling mechanism for further brain damage in these transgenic mice with astrocytic ET-1 over-expression is not yet clear. ET-1 has been shown to stimulate leukotriene (LTB4) and cytokine expression such as interleukin 6 (IL-6) after MCAO, which may contribute to brain inflammation and damage. Here, we investigated cytokine expression in brain of astrocytic ET-1 transgenic mice to determine whether the altered cytokine expressions may have lead to further brain damage in these transgenic mice. Group of transgenic and non-transgenic mice were exposed to either sham or transient MCAO. Cytokine mRNAs from these mouse brains were detected by using the RiboQuant multi-Probe Ribonuclease Protection Assay system. The brain of astrocytic ET-1 transgenic mice treated with the transient MCAO showed increased level of macrophage migration inhibitory factor (MIF), whereas IL-6 level did not show any changes. The present data suggest that altered expression of MIF in the brain of astrocytic ET-1 transgenic mice may have contributed to more severe neurologic deficits and increased brain damage after MCAO.
DescriptionPoster Session P14: Neurotrauma and Injury, Pain, Regeneration and Repair
Persistent Identifierhttp://hdl.handle.net/10722/88037
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorWu, WH-
dc.contributor.authorLo, ACY-
dc.contributor.authorChung, SK-
dc.date.accessioned2010-09-06T09:37:49Z-
dc.date.available2010-09-06T09:37:49Z-
dc.date.issued2003-
dc.identifier.citationJournal of Neurochemistry, 2003, v. 87 suppl. s1, p. 150, abstract no. P14-27-
dc.identifier.issn0022-3042-
dc.identifier.urihttp://hdl.handle.net/10722/88037-
dc.descriptionPoster Session P14: Neurotrauma and Injury, Pain, Regeneration and Repair-
dc.description.abstractPreviously, we reported that the expression of endothelin (ET-1) is induced in astrocytes and endothelial cells after cerebral hypoxia/ischemia. Recently, we also showed that transgenic mice over-expressing ET-1 in astrocytes displayed more severe neurologic deficits and increased brain infract following transient middle cerebral artery occlusion (MCAO for 2 h followed by 22 h reperfusion) compared with those of non-transgenic mice. However, the underling mechanism for further brain damage in these transgenic mice with astrocytic ET-1 over-expression is not yet clear. ET-1 has been shown to stimulate leukotriene (LTB4) and cytokine expression such as interleukin 6 (IL-6) after MCAO, which may contribute to brain inflammation and damage. Here, we investigated cytokine expression in brain of astrocytic ET-1 transgenic mice to determine whether the altered cytokine expressions may have lead to further brain damage in these transgenic mice. Group of transgenic and non-transgenic mice were exposed to either sham or transient MCAO. Cytokine mRNAs from these mouse brains were detected by using the RiboQuant multi-Probe Ribonuclease Protection Assay system. The brain of astrocytic ET-1 transgenic mice treated with the transient MCAO showed increased level of macrophage migration inhibitory factor (MIF), whereas IL-6 level did not show any changes. The present data suggest that altered expression of MIF in the brain of astrocytic ET-1 transgenic mice may have contributed to more severe neurologic deficits and increased brain damage after MCAO.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1471-4159-
dc.relation.ispartofJournal of Neurochemistry-
dc.subjectAstrocytes-
dc.subjectCytokines-
dc.subjectEndothelin-
dc.subjectIschemia-
dc.subjectTransgenic mouse-
dc.titleOver-express endothelin-1 in astrocyte lead to increased brain damage and altered cytokine expression after cerebrl ischemia-
dc.typeConference_Paper-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=87&spage=150 Suppl. 1&epage=&date=2003&atitle=Over-express+endothelin-1+in+astrocyte+lead+to+increased+brain+damage+and+altered+cytokine+expression+after+cerebrl+ischemiaen_HK
dc.identifier.emailLo, ACY: amylo@.hku.hk-
dc.identifier.emailChung, SK: skchung@hkucc.hku.hk-
dc.identifier.authorityLo, ACY=rp00425-
dc.identifier.authorityChung, SK=rp00381-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1046/j.1474-1644.2003.2175_14.x-
dc.identifier.hkuros113543-
dc.identifier.volume87-
dc.identifier.issuesuppl. s1-
dc.identifier.spage150, abstract no. P14-27-
dc.identifier.epage150, abstract no. P14-27-
dc.publisher.placeUnited Kingdom-

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