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Article: Identification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor

TitleIdentification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitor
Authors
Issue Date2002
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2002, v. 277 n. 18, p. 15237-15240 How to Cite?
AbstractNeuronal Cdc2-like kinase (Nclk) plays an important role in a variety of cellular processes, including neuronal cell differentiation, apoptosis, neuron migration, and formation of neuromuscular junction. The active kinase consists of a catalytic subunit, Cdk5, and an essential regulatory subunit, neuronal Cdk5 activator (p35 nck5a or p25 nck5a), which is expressed primarily in neurons of central nervous tissue. In our previous study using the yeast two-hybrid screening method, three novel p35 nck5a-associated proteins were isolated. Here we show that one of these proteins, called C42, specifically inhibits the activation of Cdk5 by Nck5a. Co-immunoprecipitation data suggested that C42 and p35 nck5a could form a complex within cultured mammalian cells. Deletion analysis has mapped the inhibitory domain of C42 to a region of 135 amino acids, which is conserved in Pho81, a yeast protein that inhibits the yeast cyclin-dependent protein kinase Pho85. The Pho85·Pho80 kinase complex has been shown to be the yeast functional homologue of the mammalian Cdk5/p35 nck5a kinase.
Persistent Identifierhttp://hdl.handle.net/10722/88030
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChing, YPen_HK
dc.contributor.authorPang, ASHen_HK
dc.contributor.authorLam, WHen_HK
dc.contributor.authorQi, RZen_HK
dc.contributor.authorWang, JHen_HK
dc.date.accessioned2010-09-06T09:37:44Z-
dc.date.available2010-09-06T09:37:44Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2002, v. 277 n. 18, p. 15237-15240en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88030-
dc.description.abstractNeuronal Cdc2-like kinase (Nclk) plays an important role in a variety of cellular processes, including neuronal cell differentiation, apoptosis, neuron migration, and formation of neuromuscular junction. The active kinase consists of a catalytic subunit, Cdk5, and an essential regulatory subunit, neuronal Cdk5 activator (p35 nck5a or p25 nck5a), which is expressed primarily in neurons of central nervous tissue. In our previous study using the yeast two-hybrid screening method, three novel p35 nck5a-associated proteins were isolated. Here we show that one of these proteins, called C42, specifically inhibits the activation of Cdk5 by Nck5a. Co-immunoprecipitation data suggested that C42 and p35 nck5a could form a complex within cultured mammalian cells. Deletion analysis has mapped the inhibitory domain of C42 to a region of 135 amino acids, which is conserved in Pho81, a yeast protein that inhibits the yeast cyclin-dependent protein kinase Pho85. The Pho85·Pho80 kinase complex has been shown to be the yeast functional homologue of the mammalian Cdk5/p35 nck5a kinase.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.rightsJournal of Biological Chemistry. Copyright © American Society for Biochemistry and Molecular Biology, Inc.en_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshCarrier Proteins - chemistry - metabolismen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshCyclin-Dependent Kinase 5en_HK
dc.subject.meshCyclin-Dependent Kinases - metabolismen_HK
dc.subject.meshEnzyme Inhibitors - metabolismen_HK
dc.subject.meshGlutathione Transferase - geneticsen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntracellular Signaling Peptides and Proteinsen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNerve Tissue Proteins - chemistry - metabolismen_HK
dc.subject.meshNeurons - cytology - enzymologyen_HK
dc.subject.meshRecombinant Proteins - metabolismen_HK
dc.subject.meshTransfectionen_HK
dc.titleIdentification of a neuronal Cdk5 activator-binding protein as Cdk5 inhibitoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9258&volume=277&issue=18&spage=15237&epage=15240&date=2002&atitle=Identification+of+a+neuronal+Cdk5+activator-binding+protein+as+Cdk5+inhibitoren_HK
dc.identifier.emailChing, YP:ypching@hku.hken_HK
dc.identifier.authorityChing, YP=rp00469en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1074/jbc.C200032200en_HK
dc.identifier.pmid11882646-
dc.identifier.scopuseid_2-s2.0-0037013311en_HK
dc.identifier.hkuros69114en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037013311&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume277en_HK
dc.identifier.issue18en_HK
dc.identifier.spage15237en_HK
dc.identifier.epage15240en_HK
dc.identifier.isiWOS:000175510400005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChing, YP=7005431277en_HK
dc.identifier.scopusauthoridPang, ASH=18537277000en_HK
dc.identifier.scopusauthoridLam, WH=7203022052en_HK
dc.identifier.scopusauthoridQi, RZ=7006273649en_HK
dc.identifier.scopusauthoridWang, JH=7701314238en_HK

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