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Article: Garlic inhibits microsomal triglyceride transfer protein gene expression in human liver and intestinal cell lines and in rat intestine

TitleGarlic inhibits microsomal triglyceride transfer protein gene expression in human liver and intestinal cell lines and in rat intestine
Authors
KeywordsCaco-2 cells
Garlic
Gene expression
HepG2 cells
MTP
Rats
Issue Date2002
PublisherAmerican Society for Nutrition. The Journal's web site is located at http://jn.nutrition.org
Citation
Journal Of Nutrition, 2002, v. 132 n. 6, p. 1165-1168 How to Cite?
AbstractEpidemiologic studies have suggested that fresh garlic has lipid-lowering activity. Because the microsomal triglyceride transfer protein (MTP) plays a pivotal role in the assembly and secretion of apolipoprotein B (apoB) containing lipoproteins, we evaluated the effect of garlic on the expression of the MTP gene in vitro in cell lines and in vivo in rats. Fresh garlic extract (FGE) reduced MTP mRNA levels in both the human hepatoma HepG2 and intestinal carcinoma Caco-2 cells in dose-dependent fashion; significant reductions were detected with 3 g/L FGE. Maximal 72 and 59% reductions, respectively, were observed with 6 g/L FGE. To evaluate the in vivo effect of garlic on MTP gene expression, rats were given a single oral dose of fresh garlic homogenate (FGH), with hepatic and intestinal MTP mRNA measured 3 h after dosing. Rats fed FGH had significantly (46% of the control) lower intestinal MTP mRNA levels compared with the control rats, whereas hepatic MTP mRNA levels were not affected. These results suggest a new mechanism for the hypolipidemic effect of fresh garlic. Long-term dietary supplementation of fresh garlic may exert a lipid-lowering effect partly through reducing intestinal MTP gene expression, thus suppressing the assembly and secretion of chylomicrons from intestine to the blood circulation.
Persistent Identifierhttp://hdl.handle.net/10722/88023
ISSN
2015 Impact Factor: 3.74
2015 SCImago Journal Rankings: 2.040
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, MCen_HK
dc.contributor.authorWang, EJen_HK
dc.contributor.authorLee, Cen_HK
dc.contributor.authorChin, KTen_HK
dc.contributor.authorLiu, Den_HK
dc.contributor.authorChiu, JFen_HK
dc.contributor.authorKung, HFen_HK
dc.date.accessioned2010-09-06T09:37:38Z-
dc.date.available2010-09-06T09:37:38Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Nutrition, 2002, v. 132 n. 6, p. 1165-1168en_HK
dc.identifier.issn0022-3166en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88023-
dc.description.abstractEpidemiologic studies have suggested that fresh garlic has lipid-lowering activity. Because the microsomal triglyceride transfer protein (MTP) plays a pivotal role in the assembly and secretion of apolipoprotein B (apoB) containing lipoproteins, we evaluated the effect of garlic on the expression of the MTP gene in vitro in cell lines and in vivo in rats. Fresh garlic extract (FGE) reduced MTP mRNA levels in both the human hepatoma HepG2 and intestinal carcinoma Caco-2 cells in dose-dependent fashion; significant reductions were detected with 3 g/L FGE. Maximal 72 and 59% reductions, respectively, were observed with 6 g/L FGE. To evaluate the in vivo effect of garlic on MTP gene expression, rats were given a single oral dose of fresh garlic homogenate (FGH), with hepatic and intestinal MTP mRNA measured 3 h after dosing. Rats fed FGH had significantly (46% of the control) lower intestinal MTP mRNA levels compared with the control rats, whereas hepatic MTP mRNA levels were not affected. These results suggest a new mechanism for the hypolipidemic effect of fresh garlic. Long-term dietary supplementation of fresh garlic may exert a lipid-lowering effect partly through reducing intestinal MTP gene expression, thus suppressing the assembly and secretion of chylomicrons from intestine to the blood circulation.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Nutrition. The Journal's web site is located at http://jn.nutrition.orgen_HK
dc.relation.ispartofJournal of Nutritionen_HK
dc.subjectCaco-2 cellsen_HK
dc.subjectGarlicen_HK
dc.subjectGene expressionen_HK
dc.subjectHepG2 cellsen_HK
dc.subjectMTPen_HK
dc.subjectRatsen_HK
dc.titleGarlic inhibits microsomal triglyceride transfer protein gene expression in human liver and intestinal cell lines and in rat intestineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3166&volume=132&issue=6&spage=1165&epage=8&date=2002&atitle=Garlic+inhibits+microsomal+triglyceride+transfer+protein+gene+expression+in+human+liver+and+intestinal+cell+lines+and+in+rat+intestineen_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12042427-
dc.identifier.scopuseid_2-s2.0-0036270769en_HK
dc.identifier.hkuros68872en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036270769&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume132en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1165en_HK
dc.identifier.epage1168en_HK
dc.identifier.isiWOS:000176033300011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridWang, EJ=7403414471en_HK
dc.identifier.scopusauthoridLee, C=26651829200en_HK
dc.identifier.scopusauthoridChin, KT=7202995491en_HK
dc.identifier.scopusauthoridLiu, D=21934191400en_HK
dc.identifier.scopusauthoridChiu, JF=7201501692en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK

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