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Article: A new and sensitive method for the quantification of HBV cccDNA by real-time PCR

TitleA new and sensitive method for the quantification of HBV cccDNA by real-time PCR
Authors
KeywordsHBV cccDNA
HBV quantification
Hepatitis B virus
Quantitative PCR
Issue Date2002
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2002, v. 295 n. 5, p. 1102-1107 How to Cite?
AbstractThe persistence of covalently closed circular (ccc) DNA of Hepatitis B virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. We designed a set of primers to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100-107 copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. © 2002 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88022
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHe, MLen_HK
dc.contributor.authorWu, Jen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorKung, HFen_HK
dc.date.accessioned2010-09-06T09:37:38Z-
dc.date.available2010-09-06T09:37:38Z-
dc.date.issued2002en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2002, v. 295 n. 5, p. 1102-1107en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/88022-
dc.description.abstractThe persistence of covalently closed circular (ccc) DNA of Hepatitis B virus (HBV) in liver cells is believed to be the major reason for relapse after completion of HBV antiviral therapy. Up to now, there is no sensitive method to quantify cccDNA in infected liver cells. We designed a set of primers to specifically amplify DNA fragments from HBV cccDNA but not from viral genomic DNA. A good linear range was obtained when 100-107 copies of HBV cccDNA were used as template in the quantitative real-time PCR. Not only is this method rapid, economical, highly sensitive, it can be used to monitor HBV cccDNA in infected human liver biopsies and to guide patients undergoing long-term anti-HBV therapy. © 2002 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectHBV cccDNAen_HK
dc.subjectHBV quantificationen_HK
dc.subjectHepatitis B virusen_HK
dc.subjectQuantitative PCRen_HK
dc.titleA new and sensitive method for the quantification of HBV cccDNA by real-time PCRen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=295&spage=1102&epage=1107&date=2002&atitle=A+new+and+sensitive+method+for+the+quantification+of+HBV+cccDNA+by+real-time+PCRen_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(02)00813-6en_HK
dc.identifier.pmid12135608-
dc.identifier.scopuseid_2-s2.0-0036343697en_HK
dc.identifier.hkuros68772en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036343697&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume295en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1102en_HK
dc.identifier.epage1107en_HK
dc.identifier.isiWOS:000177309700012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHe, ML=35080389700en_HK
dc.identifier.scopusauthoridWu, J=7409250054en_HK
dc.identifier.scopusauthoridChen, Y=35227073300en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK

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