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Article: Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction

TitleRapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction
Authors
KeywordsHepatitis B virus
Real-time polymerase chain reaction
Semen
Viral load
Issue Date2005
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2005, v. 11 n. 34, p. 5385-5389 How to Cite?
AbstractAim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. Methods: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. Results: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×10 7 and 1.67×10 7 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.14×10 5 and 3.02×10 5 copies of HBV DNA per mL in the semen. Conclusion: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. © 2005 The WJG Press and Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/88017
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQian, WPen_HK
dc.contributor.authorTan, YQen_HK
dc.contributor.authorChen, Yen_HK
dc.contributor.authorPeng, Yen_HK
dc.contributor.authorLi, Zen_HK
dc.contributor.authorLu, GXen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorHe, MLen_HK
dc.contributor.authorShing, LKen_HK
dc.date.accessioned2010-09-06T09:37:34Z-
dc.date.available2010-09-06T09:37:34Z-
dc.date.issued2005en_HK
dc.identifier.citationWorld Journal Of Gastroenterology, 2005, v. 11 n. 34, p. 5385-5389en_HK
dc.identifier.issn1007-9327en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88017-
dc.description.abstractAim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. Methods: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. Results: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×10 7 and 1.67×10 7 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.14×10 5 and 3.02×10 5 copies of HBV DNA per mL in the semen. Conclusion: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen. © 2005 The WJG Press and Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_HK
dc.relation.ispartofWorld Journal of Gastroenterologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectHepatitis B virusen_HK
dc.subjectReal-time polymerase chain reactionen_HK
dc.subjectSemenen_HK
dc.subjectViral loaden_HK
dc.titleRapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reactionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1007-9327&volume=11&issue=34&spage=5385&epage=9&date=2005&atitle=Rapid+quantification+of+semen+hepatitis+B+virus+DNA+by+real-time+polymerase+chain+reactionen_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3748/wjg.v11.i34.5385-
dc.identifier.pmid16149152-
dc.identifier.scopuseid_2-s2.0-26244441765en_HK
dc.identifier.hkuros107546en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26244441765&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue34en_HK
dc.identifier.spage5385en_HK
dc.identifier.epage5389en_HK
dc.identifier.isiWOS:000208100200024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridQian, WP=55106788500en_HK
dc.identifier.scopusauthoridTan, YQ=7402139346en_HK
dc.identifier.scopusauthoridChen, Y=35227073300en_HK
dc.identifier.scopusauthoridPeng, Y=7403419265en_HK
dc.identifier.scopusauthoridLi, Z=36064156900en_HK
dc.identifier.scopusauthoridLu, GX=7403460237en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridHe, ML=35080389700en_HK
dc.identifier.scopusauthoridShing, LK=54892625500en_HK

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