File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Detection and Identification of Tumor-Associated Protein Variants in Human Hepatocellular Carcinomas

TitleDetection and Identification of Tumor-Associated Protein Variants in Human Hepatocellular Carcinomas
Authors
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 2004, v. 39 n. 2, p. 540-549 How to Cite?
AbstractThe proteomic approach is a valuable tool to detect and identify proteins that are associated with cancer. In previous investigations on experimentally induced rat hepatomas, we detected aldose reductase-like protein (ARLP) as a highly significant marker protein. Our present study was intended to look for the presence of similar tumor-associated marker proteins on human hepatocellular carcinomas (HCC). We found several novel tumor-associated protein variants that represent members of the aldo-keto reductase (AKR) superfamily. Human aldose reductase-like protein-1 (hARLP-1) was the most prominent tumor-associated AKR member detected in HCC by 2-dimensional electrophoresis (2-DE) and identified by mass spectrometric fingerprinting. The enzyme was found in 4 distinct forms (hARLP-1, 36/7.4 (kd/pI); hARLP-2, 36/7.2; hARLP-3, 36/6.4; and hARLP-4, 33/7-35). In addition, a human aldose reductase-like protein (hARLP-5, 36/7.6) was identified that differed from hARLP-1 by 1 amino acid (D313N), indicating 2 allelic forms of the human aldose reductase-like gene. A novel antibody directed against common parts of the hARLPs revealed hARLP reactivity in human HCC by immunohistochemistry. Furthermore, aldose reductase (AR) was identified and characterized as a tumor-associated variant. In conclusion, in all investigated human HCCs at least one of the various types of the described tumor-associated proteins of the AKR superfamily was clearly present. Of these HCC samples, 95% were positive for hARLPs as proven by 2-DE analysis and/or by use of the antibody directed against hARLP. Thus, hARLP is a strong candidate for use as an immunohistochemical diagnostic marker of human HCC.
Persistent Identifierhttp://hdl.handle.net/10722/88014
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZeindlEberhart, Een_HK
dc.contributor.authorHaraida, Sen_HK
dc.contributor.authorLiebmann, Sen_HK
dc.contributor.authorJungblut, PRen_HK
dc.contributor.authorLamer, Sen_HK
dc.contributor.authorMayer, Den_HK
dc.contributor.authorJäger, Gen_HK
dc.contributor.authorChung, Sen_HK
dc.contributor.authorRabes, HMen_HK
dc.date.accessioned2010-09-06T09:37:31Z-
dc.date.available2010-09-06T09:37:31Z-
dc.date.issued2004en_HK
dc.identifier.citationHepatology, 2004, v. 39 n. 2, p. 540-549en_HK
dc.identifier.issn0270-9139en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88014-
dc.description.abstractThe proteomic approach is a valuable tool to detect and identify proteins that are associated with cancer. In previous investigations on experimentally induced rat hepatomas, we detected aldose reductase-like protein (ARLP) as a highly significant marker protein. Our present study was intended to look for the presence of similar tumor-associated marker proteins on human hepatocellular carcinomas (HCC). We found several novel tumor-associated protein variants that represent members of the aldo-keto reductase (AKR) superfamily. Human aldose reductase-like protein-1 (hARLP-1) was the most prominent tumor-associated AKR member detected in HCC by 2-dimensional electrophoresis (2-DE) and identified by mass spectrometric fingerprinting. The enzyme was found in 4 distinct forms (hARLP-1, 36/7.4 (kd/pI); hARLP-2, 36/7.2; hARLP-3, 36/6.4; and hARLP-4, 33/7-35). In addition, a human aldose reductase-like protein (hARLP-5, 36/7.6) was identified that differed from hARLP-1 by 1 amino acid (D313N), indicating 2 allelic forms of the human aldose reductase-like gene. A novel antibody directed against common parts of the hARLPs revealed hARLP reactivity in human HCC by immunohistochemistry. Furthermore, aldose reductase (AR) was identified and characterized as a tumor-associated variant. In conclusion, in all investigated human HCCs at least one of the various types of the described tumor-associated proteins of the AKR superfamily was clearly present. Of these HCC samples, 95% were positive for hARLPs as proven by 2-DE analysis and/or by use of the antibody directed against hARLP. Thus, hARLP is a strong candidate for use as an immunohistochemical diagnostic marker of human HCC.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_HK
dc.relation.ispartofHepatologyen_HK
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleDetection and Identification of Tumor-Associated Protein Variants in Human Hepatocellular Carcinomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0270-9139&volume=39&spage=540&epage=&date=2004&atitle=Detection+and+identification+of+tumor-associated+protein+variants+in+human+hepatocellular+carcinomasen_HK
dc.identifier.emailChung, S: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, S=rp00376en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/hep.20060en_HK
dc.identifier.pmid14768008-
dc.identifier.scopuseid_2-s2.0-1242321294en_HK
dc.identifier.hkuros91651en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1242321294&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue2en_HK
dc.identifier.spage540en_HK
dc.identifier.epage549en_HK
dc.identifier.isiWOS:000220375600034-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZeindlEberhart, E=6602635022en_HK
dc.identifier.scopusauthoridHaraida, S=6603366589en_HK
dc.identifier.scopusauthoridLiebmann, S=6602210760en_HK
dc.identifier.scopusauthoridJungblut, PR=7006422903en_HK
dc.identifier.scopusauthoridLamer, S=6701366372en_HK
dc.identifier.scopusauthoridMayer, D=7202304098en_HK
dc.identifier.scopusauthoridJäger, G=7103330370en_HK
dc.identifier.scopusauthoridChung, S=14120761600en_HK
dc.identifier.scopusauthoridRabes, HM=7006825786en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats