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Article: Characterization transgenic mice with the overexpression of ET-1 in endothelial cells as a model for experimental ischemic stroke

TitleCharacterization transgenic mice with the overexpression of ET-1 in endothelial cells as a model for experimental ischemic stroke
Authors
Issue Date2003
PublisherBlackwell Publishing Ltd.
Citation
Journal of Neurochemistry, 2003, v. 87 n. S1, p. 140 Abstract no. P13-60 How to Cite?
AbstractEndothelin-1 (ET-1), a potent vasoconstrictor, has been suggested to play a crucial role in cerebral ischemia. Previously, we have demonstrated that the ET-1 expressions were induced in astrocytic and endothelial cell after ischemic condition. Furthermore, ET-1 protected primary cultured astrocytes from hypoxic/ischemic injury. However, transgenic mice with overexpression of ET-1 in astrocytes displayed more severe neurological deficit and increased infract volume suggesting high level of astrocytic ET-1 have neurotoxic effect on neurons. In order to further investigate the role of endothelial ET-1 on neurological deficit and infarct after cerebral ischemia in vivo, several transgenic mouse lines were generated by injecting the construct which include ET cDNA and SV40 polyA under tyrosin kinase receptor-specific for endothelial cell (Tie-1) promoter which was shown to target reporter gene to endothelial cells. Over-expression of transgene, ET-1, in TET mouse brain has been characterized by reverse-transcription PCR and quantified by ribonuclease protection assay. Furthermore, the site of ET-1 over-expression in TET mice was determined by in situ hybridization. Our present TET model will serve as an ideal model for studying the pathophysiological mechanism of ischemic stroke. Keywords: endothelin-1, ischemic stroke, middle cerebral artery occlusion, nitric oxide.
Persistent Identifierhttp://hdl.handle.net/10722/88004
ISSN
2015 Impact Factor: 3.842
2015 SCImago Journal Rankings: 2.021

 

DC FieldValueLanguage
dc.contributor.authorLeung, WCen_HK
dc.contributor.authorHo, MCYen_HK
dc.contributor.authorKoon, HWen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T09:37:24Z-
dc.date.available2010-09-06T09:37:24Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal of Neurochemistry, 2003, v. 87 n. S1, p. 140 Abstract no. P13-60en_HK
dc.identifier.issn0022-3042en_HK
dc.identifier.urihttp://hdl.handle.net/10722/88004-
dc.description.abstractEndothelin-1 (ET-1), a potent vasoconstrictor, has been suggested to play a crucial role in cerebral ischemia. Previously, we have demonstrated that the ET-1 expressions were induced in astrocytic and endothelial cell after ischemic condition. Furthermore, ET-1 protected primary cultured astrocytes from hypoxic/ischemic injury. However, transgenic mice with overexpression of ET-1 in astrocytes displayed more severe neurological deficit and increased infract volume suggesting high level of astrocytic ET-1 have neurotoxic effect on neurons. In order to further investigate the role of endothelial ET-1 on neurological deficit and infarct after cerebral ischemia in vivo, several transgenic mouse lines were generated by injecting the construct which include ET cDNA and SV40 polyA under tyrosin kinase receptor-specific for endothelial cell (Tie-1) promoter which was shown to target reporter gene to endothelial cells. Over-expression of transgene, ET-1, in TET mouse brain has been characterized by reverse-transcription PCR and quantified by ribonuclease protection assay. Furthermore, the site of ET-1 over-expression in TET mice was determined by in situ hybridization. Our present TET model will serve as an ideal model for studying the pathophysiological mechanism of ischemic stroke. Keywords: endothelin-1, ischemic stroke, middle cerebral artery occlusion, nitric oxide.-
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd.en_HK
dc.relation.ispartofJournal of Neurochemistryen_HK
dc.rightsJournal of Neurochemistry. Copyright © Blackwell Publishing Ltd.en_HK
dc.titleCharacterization transgenic mice with the overexpression of ET-1 in endothelial cells as a model for experimental ischemic strokeen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3042&volume=87&spage=140 Suppl. 1&epage=&date=2003&atitle=Characterization+transgenic+mice+with+the+overexpression+of+ET-1+in+endothelial+cells+as+a+model+for+experimental+ichemic+strokeen_HK
dc.identifier.emailLeung, WC: just@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1474-1644.2003.2175_13.x-
dc.identifier.hkuros113549en_HK

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