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Article: Transgenic mice over-expressing endothelin-1 in testis transactivated by a Cre/loxP system showed decreased testicular capillary blood flow

TitleTransgenic mice over-expressing endothelin-1 in testis transactivated by a Cre/loxP system showed decreased testicular capillary blood flow
Authors
KeywordsConditional
EF1α
EIIA-cre
Transgenic mice
Two-tiered
Issue Date2004
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0962-8819
Citation
Transgenic Research, 2004, v. 13 n. 2, p. 119-134 How to Cite?
AbstractIt is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express ET-1 in a tissue-specific manner. In such a strategy a single transgenic mouse line, ELSE, was initially generated where a general promoter, human elongation factor 1α (hEF1α) promoter, was used to drive the expression of a loxP-flanked sequence containing the lacZ reporter gene and a STOP cassette before the ET-1 cDNA, the recombinational competency of which was confirmed in an Escherichia coli test system. In ELSE mice, expression of the reporter lacZ was limited to spermatozoa and spermatogonia as well as Sertoli, Leydig and endothelial cells in the testis, thus confirming the suitability of these mice for the generation of testes-limited ET-1 expression. To generate transgenic progeny with ET-1 over-expression in the testis (successful recombination, ELSE/ELT), ELSE mice were mated with EIIa-cre mice expressing Cre recombinase in pre-implantation mouse embryos. These ELSE/ELT mice exhibiting testis-specific ET-1 over-expression had normal reproductive function and showed no obvious alterations in gross testicular morphology. Although over-expression of ET-1 leads to reduction of testicular blood flow, young adult ELSE/ELT mice showed no obvious signs of inflammation, fibrosis or abnormal proliferation of cells in the testes of young ELSE/ELT mice by histochemical analyses.
Persistent Identifierhttp://hdl.handle.net/10722/87991
ISSN
2015 Impact Factor: 2.054
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, ACYen_HK
dc.contributor.authorFung, MKLen_HK
dc.contributor.authorAu, CLen_HK
dc.contributor.authorChan, TSKen_HK
dc.contributor.authorSauer, Ben_HK
dc.contributor.authorChung, SSMen_HK
dc.contributor.authorChung, SKen_HK
dc.date.accessioned2010-09-06T09:37:14Z-
dc.date.available2010-09-06T09:37:14Z-
dc.date.issued2004en_HK
dc.identifier.citationTransgenic Research, 2004, v. 13 n. 2, p. 119-134en_HK
dc.identifier.issn0962-8819en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87991-
dc.description.abstractIt is generally believed that too high or low levels of endothelin-1 (ET-1), a strong vasoconstrictor, may be detrimental to animals. Therefore, in order to understand the in vivo function of ET-1, we used a conditional transgenic approach, Cre/loxP recombination system, to generate transgenic mice that over-express ET-1 in a tissue-specific manner. In such a strategy a single transgenic mouse line, ELSE, was initially generated where a general promoter, human elongation factor 1α (hEF1α) promoter, was used to drive the expression of a loxP-flanked sequence containing the lacZ reporter gene and a STOP cassette before the ET-1 cDNA, the recombinational competency of which was confirmed in an Escherichia coli test system. In ELSE mice, expression of the reporter lacZ was limited to spermatozoa and spermatogonia as well as Sertoli, Leydig and endothelial cells in the testis, thus confirming the suitability of these mice for the generation of testes-limited ET-1 expression. To generate transgenic progeny with ET-1 over-expression in the testis (successful recombination, ELSE/ELT), ELSE mice were mated with EIIa-cre mice expressing Cre recombinase in pre-implantation mouse embryos. These ELSE/ELT mice exhibiting testis-specific ET-1 over-expression had normal reproductive function and showed no obvious alterations in gross testicular morphology. Although over-expression of ET-1 leads to reduction of testicular blood flow, young adult ELSE/ELT mice showed no obvious signs of inflammation, fibrosis or abnormal proliferation of cells in the testes of young ELSE/ELT mice by histochemical analyses.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0962-8819en_HK
dc.relation.ispartofTransgenic Researchen_HK
dc.subjectConditionalen_HK
dc.subjectEF1αen_HK
dc.subjectEIIA-creen_HK
dc.subjectTransgenic miceen_HK
dc.subjectTwo-tiereden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCapillaries - physiologyen_HK
dc.subject.meshEndothelin-1 - genetics - metabolismen_HK
dc.subject.meshGenes, Reporter - geneticsen_HK
dc.subject.meshIntegrases - genetics - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshPeptide Elongation Factor 1 - geneticsen_HK
dc.subject.meshRNA, Messenger - analysisen_HK
dc.subject.meshTestis - blood supply - metabolism - ultrastructureen_HK
dc.subject.meshUp-Regulationen_HK
dc.subject.meshViral Proteins - genetics - metabolismen_HK
dc.subject.meshbeta-Galactosidase - analysisen_HK
dc.titleTransgenic mice over-expressing endothelin-1 in testis transactivated by a Cre/loxP system showed decreased testicular capillary blood flowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0962-8819&volume=13&spage=119&epage=134&date=2004&atitle=Transgenic+mice+over-expressing+endothelin-1+in+testis+transactivated+by+a+cre/loxP+system+showed+decreased+testicular+capillary+blood+flowen_HK
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_HK
dc.identifier.emailChung, SSM: smchung@hkucc.hku.hken_HK
dc.identifier.emailChung, SK: skchung@hkucc.hku.hken_HK
dc.identifier.authorityLo, ACY=rp00425en_HK
dc.identifier.authorityChung, SSM=rp00376en_HK
dc.identifier.authorityChung, SK=rp00381en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/B:TRAG.0000026072.01351.10en_HK
dc.identifier.pmid15198200-
dc.identifier.scopuseid_2-s2.0-3242877050en_HK
dc.identifier.hkuros91216en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3242877050&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue2en_HK
dc.identifier.spage119en_HK
dc.identifier.epage134en_HK
dc.identifier.isiWOS:000221121900004-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLo, ACY=7102780640en_HK
dc.identifier.scopusauthoridFung, MKL=8718040400en_HK
dc.identifier.scopusauthoridAu, CL=7102805672en_HK
dc.identifier.scopusauthoridChan, TSK=7402687396en_HK
dc.identifier.scopusauthoridSauer, B=36513795500en_HK
dc.identifier.scopusauthoridChung, SSM=14120761600en_HK
dc.identifier.scopusauthoridChung, SK=7404292976en_HK

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