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Article: Peroxynitrite induces apoptosis of HL-60 cells by activation of a caspase-3 family protease

TitlePeroxynitrite induces apoptosis of HL-60 cells by activation of a caspase-3 family protease
Authors
KeywordsCaspase cysteine protease
Leukemia cells
Programmed cell death
Reactive nitrogen species
Issue Date1998
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 1998, v. 274 n. 4 43-4, p. C855-C860 How to Cite?
AbstractApoptosis is an active process critical for the homeostasis of organisms. Enzymes of the caspase family are responsible for executing this process. We have previously shown that peroxynitrite (ONOO-), a biological product generated from the interaction of nitric oxide and superoxide, induces apoptosis of HL-60 cells. The aim of this study was to elucidate the mechanisms involved in the execution process of peroxynitrite-induced apoptosis. Proteolytic cleavage of poly(ADP-ribose) polymerase, an indication of caspase-3 family protease activation and an early biochemical event accompanying apoptosis, was observed in a time-dependent manner during peroxynitrite-induced apoptosis of HL-60 cells. Activation of caspase-3 during peroxynitrite-induced apoptosis was substantiated by monitoring proteolysis of the caspase-3 proenzyme and by measuring caspase-3 activity with a flurogenic substrate. Furthermore, pretreatment of HL-60 cells with N- acetyl-Asp-Glu-Val-Asp-aldehyde, a specific inhibitor of caspase-3, but not N-acetyl-Tyr-Val-Ala-Asp-aldehyde, a specific inhibitor of caspase-1, decreased peroxynitrite-induced apoptosis. These results suggest that the activation of a caspase-3 family protease is essential for initiating the execution process of peroxynitrite-induced apoptosis of HL-60 cells.
Persistent Identifierhttp://hdl.handle.net/10722/87986
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, KTen_HK
dc.contributor.authorXue, JYen_HK
dc.contributor.authorLin, MCen_HK
dc.contributor.authorSpokas, EGen_HK
dc.contributor.authorSun, FFen_HK
dc.contributor.authorWong, PYKen_HK
dc.date.accessioned2010-09-06T09:37:09Z-
dc.date.available2010-09-06T09:37:09Z-
dc.date.issued1998en_HK
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 1998, v. 274 n. 4 43-4, p. C855-C860en_HK
dc.identifier.issn0363-6143en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87986-
dc.description.abstractApoptosis is an active process critical for the homeostasis of organisms. Enzymes of the caspase family are responsible for executing this process. We have previously shown that peroxynitrite (ONOO-), a biological product generated from the interaction of nitric oxide and superoxide, induces apoptosis of HL-60 cells. The aim of this study was to elucidate the mechanisms involved in the execution process of peroxynitrite-induced apoptosis. Proteolytic cleavage of poly(ADP-ribose) polymerase, an indication of caspase-3 family protease activation and an early biochemical event accompanying apoptosis, was observed in a time-dependent manner during peroxynitrite-induced apoptosis of HL-60 cells. Activation of caspase-3 during peroxynitrite-induced apoptosis was substantiated by monitoring proteolysis of the caspase-3 proenzyme and by measuring caspase-3 activity with a flurogenic substrate. Furthermore, pretreatment of HL-60 cells with N- acetyl-Asp-Glu-Val-Asp-aldehyde, a specific inhibitor of caspase-3, but not N-acetyl-Tyr-Val-Ala-Asp-aldehyde, a specific inhibitor of caspase-1, decreased peroxynitrite-induced apoptosis. These results suggest that the activation of a caspase-3 family protease is essential for initiating the execution process of peroxynitrite-induced apoptosis of HL-60 cells.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_HK
dc.subjectCaspase cysteine proteaseen_HK
dc.subjectLeukemia cellsen_HK
dc.subjectProgrammed cell deathen_HK
dc.subjectReactive nitrogen speciesen_HK
dc.titlePeroxynitrite induces apoptosis of HL-60 cells by activation of a caspase-3 family proteaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9513&volume=274&spage=C855&epage=860&date=1998&atitle=Peroxynitrite+induces+apoptosis+of+HL-60+cells+by+activation+of+a+caspase-3+family+proteaseen_HK
dc.identifier.emailLin, MC:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityLin, MC=rp00746en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid9575780-
dc.identifier.scopuseid_2-s2.0-0031980291en_HK
dc.identifier.hkuros46080en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031980291&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume274en_HK
dc.identifier.issue4 43-4en_HK
dc.identifier.spageC855en_HK
dc.identifier.epageC860en_HK
dc.identifier.isiWOS:000072850000002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLin, KT=7403967674en_HK
dc.identifier.scopusauthoridXue, JY=14057428500en_HK
dc.identifier.scopusauthoridLin, MC=7404816359en_HK
dc.identifier.scopusauthoridSpokas, EG=6701462653en_HK
dc.identifier.scopusauthoridSun, FF=7401804215en_HK
dc.identifier.scopusauthoridWong, PYK=7403980650en_HK

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