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Article: Coculture of human oviductal cells maintains mitochondrial function and decreases caspase activity of cleavage-stage mouse embryos

TitleCoculture of human oviductal cells maintains mitochondrial function and decreases caspase activity of cleavage-stage mouse embryos
Authors
KeywordsCaspase
Coculture
Embryo
Mitochondria
Oviduct
Issue Date2003
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2003, v. 80 n. 1, p. 178-183 How to Cite?
AbstractObjective: To investigate the mitochondrial function and caspase activity in mouse embryos after human oviductal cell coculture. Design: Experimental laboratory study. Setting: University gynecology unit. Animal(s): MF-1 (female); BALB/c (male) mice. Intervention(s): Mouse embryos were cocultured with human oviductal cells. Main Outcome Measure(s): Mitochondrial transmembrane potential (Δψ m) and caspase activity. Result(s): Compared to embryos after coculture in Chatot-Ziomek-Bavister (CZB) medium supplemented with 0.5 mg/mL of BSA (CZB), Δψm of embryos cultured in CZB was significantly lower at the two-cell (CZB, 2.04 ± 0.412; coculture, 4.34 ± 0.563) and morula (CZB, 6.06 ± 0.548; coculture, 7.12 ± 0.568) stages. Cocultured embryos and in vivo developed embryos had comparable Δψm. Caspase activity was not detected in unfragmented cleavage-stage embryos and morula developed in vivo. In vitro cultured morula possessed caspase activity. The activity was significantly reduced in the cocultured morula. Conclusion(s): Human oviductal cells maintained the mitochondria function in terms of mitochondrial transmembrane potential and decreased the caspase activity to improve the development of mouse embryo. © 2003 by American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87482
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, JSen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:30:13Z-
dc.date.available2010-09-06T09:30:13Z-
dc.date.issued2003en_HK
dc.identifier.citationFertility And Sterility, 2003, v. 80 n. 1, p. 178-183en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87482-
dc.description.abstractObjective: To investigate the mitochondrial function and caspase activity in mouse embryos after human oviductal cell coculture. Design: Experimental laboratory study. Setting: University gynecology unit. Animal(s): MF-1 (female); BALB/c (male) mice. Intervention(s): Mouse embryos were cocultured with human oviductal cells. Main Outcome Measure(s): Mitochondrial transmembrane potential (Δψ m) and caspase activity. Result(s): Compared to embryos after coculture in Chatot-Ziomek-Bavister (CZB) medium supplemented with 0.5 mg/mL of BSA (CZB), Δψm of embryos cultured in CZB was significantly lower at the two-cell (CZB, 2.04 ± 0.412; coculture, 4.34 ± 0.563) and morula (CZB, 6.06 ± 0.548; coculture, 7.12 ± 0.568) stages. Cocultured embryos and in vivo developed embryos had comparable Δψm. Caspase activity was not detected in unfragmented cleavage-stage embryos and morula developed in vivo. In vitro cultured morula possessed caspase activity. The activity was significantly reduced in the cocultured morula. Conclusion(s): Human oviductal cells maintained the mitochondria function in terms of mitochondrial transmembrane potential and decreased the caspase activity to improve the development of mouse embryo. © 2003 by American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.-
dc.subjectCaspaseen_HK
dc.subjectCocultureen_HK
dc.subjectEmbryoen_HK
dc.subjectMitochondriaen_HK
dc.subjectOviducten_HK
dc.titleCoculture of human oviductal cells maintains mitochondrial function and decreases caspase activity of cleavage-stage mouse embryosen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=80&issue=1&spage=178&epage=183&date=2003&atitle=Coculture+of+human+oviductal+cells+maintains+mitochondrial+function+and+decreases+caspase+activity+of+cleavage-stage+mouse+embryosen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(03)00570-3en_HK
dc.identifier.pmid12849821-
dc.identifier.scopuseid_2-s2.0-0038045076en_HK
dc.identifier.hkuros87623en_HK
dc.identifier.hkuros82024-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038045076&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue1en_HK
dc.identifier.spage178en_HK
dc.identifier.epage183en_HK
dc.identifier.isiWOS:000184170200029-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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