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Article: Effects of human follicular fluid on spermatozoa that have been cocultured with human oviductal cells

TitleEffects of human follicular fluid on spermatozoa that have been cocultured with human oviductal cells
Authors
KeywordsCoculture
Follicular fluid
Oviductal cells
Sperm functions
Issue Date1999
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 1999, v. 72 n. 6, p. 1079-1084 How to Cite?
AbstractObjective: To investigate the sequential effects of human oviductal cells and human follicular fluid (hFF) on various sperm functions. Design: Laboratory experimental study. Setting: University gynecology unit. Patient(s): Fallopian tubes were from patients undergoing tubal ligation or hysterectomy. Semen was from men attending the subfertility clinics. Intervention(s): Spermatozoa were treated with [1] 6 hours in Earle's balanced salt solution (EBSS-BSA; control); [2] 5 hours in EBSS-BSA and 1 hour with hFF (hFF); [3] 5 hours with oviductal cells and 1 hour in EBSS-BSA (coculture); and [4] 5 hours with oviductal cells and 1 hour with hFF (sequential). Main Outcome Measure(s): Motility, acrosome reaction, zona binding, and oocyte fusion. Result(s): Groups II and III spermatozoa had similar motility and were better than that of group I. Group IV displayed higher motility parameters than the other groups. Human follicular fluid induced acrosome reaction. The incidence of acrosome reaction in group IV was significantly lower than that in group II. Group III did not affect the acrosome reaction. Spermatozoa in groups II-IV had lower zona binding capacity than those in group I. Human follicular fluid stimulated oocyte penetration, whereas oviductal cells suppressed this effect of hFF. Conclusion(s): Oviductal cells maintained the fertilizing capacity of spermatozoa, whereas hFF facilitated the fertilization process of oviductal spermatozoa.
Persistent Identifierhttp://hdl.handle.net/10722/87465
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:30:00Z-
dc.date.available2010-09-06T09:30:00Z-
dc.date.issued1999en_HK
dc.identifier.citationFertility And Sterility, 1999, v. 72 n. 6, p. 1079-1084en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87465-
dc.description.abstractObjective: To investigate the sequential effects of human oviductal cells and human follicular fluid (hFF) on various sperm functions. Design: Laboratory experimental study. Setting: University gynecology unit. Patient(s): Fallopian tubes were from patients undergoing tubal ligation or hysterectomy. Semen was from men attending the subfertility clinics. Intervention(s): Spermatozoa were treated with [1] 6 hours in Earle's balanced salt solution (EBSS-BSA; control); [2] 5 hours in EBSS-BSA and 1 hour with hFF (hFF); [3] 5 hours with oviductal cells and 1 hour in EBSS-BSA (coculture); and [4] 5 hours with oviductal cells and 1 hour with hFF (sequential). Main Outcome Measure(s): Motility, acrosome reaction, zona binding, and oocyte fusion. Result(s): Groups II and III spermatozoa had similar motility and were better than that of group I. Group IV displayed higher motility parameters than the other groups. Human follicular fluid induced acrosome reaction. The incidence of acrosome reaction in group IV was significantly lower than that in group II. Group III did not affect the acrosome reaction. Spermatozoa in groups II-IV had lower zona binding capacity than those in group I. Human follicular fluid stimulated oocyte penetration, whereas oviductal cells suppressed this effect of hFF. Conclusion(s): Oviductal cells maintained the fertilizing capacity of spermatozoa, whereas hFF facilitated the fertilization process of oviductal spermatozoa.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectCocultureen_HK
dc.subjectFollicular fluiden_HK
dc.subjectOviductal cellsen_HK
dc.subjectSperm functionsen_HK
dc.titleEffects of human follicular fluid on spermatozoa that have been cocultured with human oviductal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=72&issue=6&spage=1079&epage=1084&date=1999&atitle=Effects+of+human+follicular+fluid+on+spermatozoa+that+have+been+cocultured+with+human+oviductal+cellsen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(99)00420-3en_HK
dc.identifier.pmid10593385-
dc.identifier.scopuseid_2-s2.0-0032730798en_HK
dc.identifier.hkuros48585en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032730798&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume72en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1079en_HK
dc.identifier.epage1084en_HK
dc.identifier.isiWOS:000083904500023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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