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Article: Separation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis and its application in capillary electrophoresis-based immunoassay

TitleSeparation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis and its application in capillary electrophoresis-based immunoassay
Authors
KeywordsBovine serum albumin
Monoclonal antibodies
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromb
Citation
Journal Of Chromatography B: Biomedical Sciences And Applications, 1999, v. 731 n. 2, p. 389-394 How to Cite?
AbstractA non-competitive immunoassay was performed by sodium dodecyl sulfate-capillary gel electrophoresis with UV detection using bovine serum albumin (BSA) and monoclonal anti-BSA. BSA, anti-BSA and their immunocomplexes were well resolved under non-denaturing conditions. A linear calibration curve was obtained and can be used for the quantification of anti-BSA. The limit of detection of anti-BSA was 0.1 μM under the present conditions. Compared with capillary zone electrophoresis, we believed that this method has the potential to be used as a more general format for performing capillary electrophoresis-based immunoassay of medium- and large-sized analytes. Copyright (C) 1999 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/87422
ISSN
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorOu, JPen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:29:27Z-
dc.date.available2010-09-06T09:29:27Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Chromatography B: Biomedical Sciences And Applications, 1999, v. 731 n. 2, p. 389-394en_HK
dc.identifier.issn1387-2273en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87422-
dc.description.abstractA non-competitive immunoassay was performed by sodium dodecyl sulfate-capillary gel electrophoresis with UV detection using bovine serum albumin (BSA) and monoclonal anti-BSA. BSA, anti-BSA and their immunocomplexes were well resolved under non-denaturing conditions. A linear calibration curve was obtained and can be used for the quantification of anti-BSA. The limit of detection of anti-BSA was 0.1 μM under the present conditions. Compared with capillary zone electrophoresis, we believed that this method has the potential to be used as a more general format for performing capillary electrophoresis-based immunoassay of medium- and large-sized analytes. Copyright (C) 1999 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jchromben_HK
dc.relation.ispartofJournal of Chromatography B: Biomedical Sciences and Applicationsen_HK
dc.rightsJournal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences. Copyright © Elsevier BV.en_HK
dc.subjectBovine serum albuminen_HK
dc.subjectMonoclonal antibodiesen_HK
dc.titleSeparation of bovine serum albumin and its monoclonal antibody from their immunocomplexes by sodium dodecyl sulfate-capillary gel electrophoresis and its application in capillary electrophoresis-based immunoassayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1570-0232&volume=731&spage=389&epage=394&date=1999&atitle=Separation+of+bovine+serum+albumin+and+its+monoclonal+antibody+from+their+immunocomplexes+by+sodium+dodecyl+sulfate-capillary+gel+electrophoresis+and+its+application+in+capillary+electrophoresis-based+immunoassayen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0378-4347(99)00234-0en_HK
dc.identifier.pmid10510794en_HK
dc.identifier.scopuseid_2-s2.0-0032765282en_HK
dc.identifier.hkuros47687en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032765282&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume731en_HK
dc.identifier.issue2en_HK
dc.identifier.spage389en_HK
dc.identifier.epage394en_HK
dc.identifier.isiWOS:000082472800026-
dc.identifier.scopusauthoridOu, JP=7202845297en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.citeulike2407526-

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