File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Human oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryo

TitleHuman oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryo
Authors
KeywordsCoculture
Embryos
Embryotrophic factor
Oviductal cells
Issue Date1995
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 1995, v. 10 n. 10, p. 2781-2786 How to Cite?
AbstractThe coculture effects of human oviductal cells on mouse embryo development in vitro were studied. Pronuclear stage mouse zygotes were cocultured with different cell types, or then cultured either in medium alone (control), spent medium from oviductal cell culture (conditioned medium) or high molecular weight fractions ( > 10 and > 100 kDa) of the conditioned medium (reconstituted medium). Embryotrophic activities were compared between these groups in terms of percentage of morula and blastocyst formation, and cell count at the blastocyst stage. The mouse embryos developed better in oviductal cell coculture than in fibroblast coculture and medium alone culture. Conditioned medium and its reconstituted medium also provided a significant enhancement of embryo development in vitro when compared with the control medium culture, suggesting the production of high molecular weight embryotrophic factor(s) by the oviductal cells. The high molecular weight embryotrophic activity accumulated with the duration of conditioning could be serially diluted, and was abolished by heat or trypsin treatment. Replacing bovine serum albumin with polyvinyl alcohol in the culture medium did not affect the production of this high molecular weight embryotrophic activity by oviductal cells.
Persistent Identifierhttp://hdl.handle.net/10722/87419
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, LPSen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:29:25Z-
dc.date.available2010-09-06T09:29:25Z-
dc.date.issued1995en_HK
dc.identifier.citationHuman Reproduction, 1995, v. 10 n. 10, p. 2781-2786en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87419-
dc.description.abstractThe coculture effects of human oviductal cells on mouse embryo development in vitro were studied. Pronuclear stage mouse zygotes were cocultured with different cell types, or then cultured either in medium alone (control), spent medium from oviductal cell culture (conditioned medium) or high molecular weight fractions ( > 10 and > 100 kDa) of the conditioned medium (reconstituted medium). Embryotrophic activities were compared between these groups in terms of percentage of morula and blastocyst formation, and cell count at the blastocyst stage. The mouse embryos developed better in oviductal cell coculture than in fibroblast coculture and medium alone culture. Conditioned medium and its reconstituted medium also provided a significant enhancement of embryo development in vitro when compared with the control medium culture, suggesting the production of high molecular weight embryotrophic factor(s) by the oviductal cells. The high molecular weight embryotrophic activity accumulated with the duration of conditioning could be serially diluted, and was abolished by heat or trypsin treatment. Replacing bovine serum albumin with polyvinyl alcohol in the culture medium did not affect the production of this high molecular weight embryotrophic activity by oviductal cells.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectCocultureen_HK
dc.subjectEmbryosen_HK
dc.subjectEmbryotrophic factoren_HK
dc.subjectOviductal cellsen_HK
dc.titleHuman oviductal cells produce high molecular weight factor(s) that improves the development of mouse embryoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=10&spage=2781&epage=2786&date=1996&atitle=Human+oviductal+cells+produce+high+molecular+weight+factor(s)+that+improves+the+development+of+mouse+embryoen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8567813-
dc.identifier.scopuseid_2-s2.0-0028811184en_HK
dc.identifier.hkuros14315en_HK
dc.identifier.volume10en_HK
dc.identifier.issue10en_HK
dc.identifier.spage2781en_HK
dc.identifier.epage2786en_HK
dc.identifier.isiWOS:A1995TF39300060-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLiu, LPS=19335129300en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats