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Article: Sandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and diode laser induced fluorescence detection

TitleSandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and diode laser induced fluorescence detection
Authors
Wang, QLuo, GWang, YYeung, WSB
Issue Date2000
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10826076.asp
Citation
Journal Of Liquid Chromatography And Related Technologies, 2000, v. 23 n. 10, p. 1489-1498 How to Cite?
DOI: http://dx.doi.org/10.1081/JLC-100100429
AbstractA sandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and LIF detection was described. Monoclonal anti-bovine serum albumin (BSA) was used as a model to test the performance of the system. Cy5 was used as the fluorescent tag and the 635 nm line of a diode laser was used as the excited source. Capillaries with inner diameter of 150 μm were packed with recombinant protein G bound perfusive support and used to capture the immunocomplexes, which were subsequently desorbed by an acidic buffer and then detected using LIF. The results demonstrated the feasibility to perform sandwich immunoassay using this system.
Persistent Identifierhttp://hdl.handle.net/10722/87414
ISSN
1082-6076
2023 Impact Factor: 1.0
2023 SCImago Journal Rankings: 0.262
ISI Accession Number ID
WOS:000087770500003
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorWang, Qen_HK
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:29:21Z-
dc.date.available2010-09-06T09:29:21Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Liquid Chromatography And Related Technologies, 2000, v. 23 n. 10, p. 1489-1498en_HK
dc.identifier.issn1082-6076en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87414-
dc.description.abstractA sandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and LIF detection was described. Monoclonal anti-bovine serum albumin (BSA) was used as a model to test the performance of the system. Cy5 was used as the fluorescent tag and the 635 nm line of a diode laser was used as the excited source. Capillaries with inner diameter of 150 μm were packed with recombinant protein G bound perfusive support and used to capture the immunocomplexes, which were subsequently desorbed by an acidic buffer and then detected using LIF. The results demonstrated the feasibility to perform sandwich immunoassay using this system.en_HK
dc.languageengen_HK
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/10826076.aspen_HK
dc.relation.ispartofJournal of Liquid Chromatography and Related Technologiesen_HK
dc.titleSandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and diode laser induced fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0148-3919&volume=23&issue=10&spage=1489&epage=1498&date=2000&atitle=Sandwich+immunoassay+for+monoclonal+antibody+using+protein+G+immunoaffinity+capillary+chromatography+and+diode+laser+induced+fluorescence+detectionen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1081/JLC-100100429en_HK
dc.identifier.scopuseid_2-s2.0-0034088658en_HK
dc.identifier.hkuros59256en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034088658&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1489en_HK
dc.identifier.epage1498en_HK
dc.identifier.isiWOS:000087770500003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Q=7406915351en_HK
dc.identifier.scopusauthoridLuo, G=7401536462en_HK
dc.identifier.scopusauthoridWang, Y=13310049900en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.issnl1082-6076-

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