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Article: Profiles of sperm morphology and motility after discontinuous multiple-step Percoll density gradient centrifugation

TitleProfiles of sperm morphology and motility after discontinuous multiple-step Percoll density gradient centrifugation
Authors
Keywordshuman
morphology
motility
Percoll gradient centrifugation
spermatozoa
Issue Date1996
PublisherBlackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/AND
Citation
Andrologia, 1996, v. 28 n. 2, p. 127-131 How to Cite?
AbstractMorphology and motility are important parameters for assessing the fertilizing capacity of spermatozoa. This investigation reports a systematic study on the profiles of these parameters after Percoll gradient centrifugation. Spermatozoa from normal human semen were fractionated by discontinuous Percoll gradients (30%, 45%, 75%, 90%). Spermatozoa washed with Earle's balanced salt solution were used as a control. After centrifugation, sperm morphology was evaluated according to strict criteria; motility was assessed by a computer-assisted semen analysis system. The results showed that the percentage of spermatozoa with normal morphology increased, while those with severe morphological defects decreased, as the density of Percoll increased. The percentages of motile spermatozoa and hyperactivated spermatozoa, and the velocity and amplitude of lateral head displacement of the spermatozoa were significantly higher in the 75% and 90% Percoll fractions than that in the 30% and 45% ones, and in the control. These results demonstrated that Percoll density gradient centrifugation enriched spermatozoa in terms of morphology and motility.
Persistent Identifierhttp://hdl.handle.net/10722/87412
ISSN
2015 Impact Factor: 1.441
2015 SCImago Journal Rankings: 0.528
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorNg, Ven_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2010-09-06T09:29:20Z-
dc.date.available2010-09-06T09:29:20Z-
dc.date.issued1996en_HK
dc.identifier.citationAndrologia, 1996, v. 28 n. 2, p. 127-131en_HK
dc.identifier.issn0303-4569en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87412-
dc.description.abstractMorphology and motility are important parameters for assessing the fertilizing capacity of spermatozoa. This investigation reports a systematic study on the profiles of these parameters after Percoll gradient centrifugation. Spermatozoa from normal human semen were fractionated by discontinuous Percoll gradients (30%, 45%, 75%, 90%). Spermatozoa washed with Earle's balanced salt solution were used as a control. After centrifugation, sperm morphology was evaluated according to strict criteria; motility was assessed by a computer-assisted semen analysis system. The results showed that the percentage of spermatozoa with normal morphology increased, while those with severe morphological defects decreased, as the density of Percoll increased. The percentages of motile spermatozoa and hyperactivated spermatozoa, and the velocity and amplitude of lateral head displacement of the spermatozoa were significantly higher in the 75% and 90% Percoll fractions than that in the 30% and 45% ones, and in the control. These results demonstrated that Percoll density gradient centrifugation enriched spermatozoa in terms of morphology and motility.en_HK
dc.languageengen_HK
dc.publisherBlackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/ANDen_HK
dc.relation.ispartofAndrologiaen_HK
dc.subjecthumanen_HK
dc.subjectmorphologyen_HK
dc.subjectmotilityen_HK
dc.subjectPercoll gradient centrifugationen_HK
dc.subjectspermatozoaen_HK
dc.titleProfiles of sperm morphology and motility after discontinuous multiple-step Percoll density gradient centrifugationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-4569&volume=28&spage=127&epage=131&date=1996&atitle=Profiles+of+sperm+morphology+and+motility+after+discontinuous+multiple+step+Percoll+density+gradient+centrifugationen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8849048-
dc.identifier.scopuseid_2-s2.0-0029931046en_HK
dc.identifier.hkuros14304en_HK
dc.identifier.hkuros23019-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029931046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue2en_HK
dc.identifier.spage127en_HK
dc.identifier.epage131en_HK
dc.identifier.isiWOS:A1996UB18400009-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridNg, V=7102162970en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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