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Article: Identification of mRNAs that are up-regulated after fertilization in the murine zygote by suppression subtractive hybridization

TitleIdentification of mRNAs that are up-regulated after fertilization in the murine zygote by suppression subtractive hybridization
Authors
KeywordsMouse embryos
Oocyte
Preimplantation
Suppression subtractive hybridization
Zygotic gene activation
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2003, v. 304 n. 1, p. 60-66 How to Cite?
AbstractTranscriptions occur in mouse preimplantation embryos as early as one-cell stage. However, our understanding on gene expression at this stage is lacking. The present study applied suppression subtractive hybridization (SSH) to compared gene expression profiles of mouse zygote and oocyte. Forty-four differentially expressed genes were selected and shown positive signals by reverse dot-blot hybridization. DNA sequences comparison of these putative clones with the GenBank/EMBL databases using BLAST search identified 38 clones with 90% identity to known genes and six novel clones with less than 70% homology to the databases. Eleven out of the 44 differentially expressed clones were either originally isolated from male embryo or testis-specific genes, suggesting that these genes may be derived from paternal genome. Five differentially expressed genes of interest, including bromodomain-containing protein BP75, spindlin, radixin, pituitary tumor-transforming gene (PTTG), and proteoglycan core protein (serglycin) were further studied by semi-quantitative RT-PCR. It is noted that spindlin which involves in cell division is highly expressed in zygote, suggesting that this protein may play an important role in zygotic gene activation (ZGA) and early stage development in 1-cell stage mouse embryos. © 2003 Elsevier Science (USA). All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87380
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorXu, JSen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLee, KFen_HK
dc.date.accessioned2010-09-06T09:28:56Z-
dc.date.available2010-09-06T09:28:56Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 304 n. 1, p. 60-66en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87380-
dc.description.abstractTranscriptions occur in mouse preimplantation embryos as early as one-cell stage. However, our understanding on gene expression at this stage is lacking. The present study applied suppression subtractive hybridization (SSH) to compared gene expression profiles of mouse zygote and oocyte. Forty-four differentially expressed genes were selected and shown positive signals by reverse dot-blot hybridization. DNA sequences comparison of these putative clones with the GenBank/EMBL databases using BLAST search identified 38 clones with 90% identity to known genes and six novel clones with less than 70% homology to the databases. Eleven out of the 44 differentially expressed clones were either originally isolated from male embryo or testis-specific genes, suggesting that these genes may be derived from paternal genome. Five differentially expressed genes of interest, including bromodomain-containing protein BP75, spindlin, radixin, pituitary tumor-transforming gene (PTTG), and proteoglycan core protein (serglycin) were further studied by semi-quantitative RT-PCR. It is noted that spindlin which involves in cell division is highly expressed in zygote, suggesting that this protein may play an important role in zygotic gene activation (ZGA) and early stage development in 1-cell stage mouse embryos. © 2003 Elsevier Science (USA). All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectMouse embryosen_HK
dc.subjectOocyteen_HK
dc.subjectPreimplantationen_HK
dc.subjectSuppression subtractive hybridizationen_HK
dc.subjectZygotic gene activationen_HK
dc.titleIdentification of mRNAs that are up-regulated after fertilization in the murine zygote by suppression subtractive hybridizationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=304&spage=60&epage=66&date=2003&atitle=Identification+of+mRNAs+that+are+up-regulated+after+fertilization+in+the+murine+zygote+by+suppression+subtractive+hybridizationen_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(03)00537-0en_HK
dc.identifier.pmid12705884-
dc.identifier.scopuseid_2-s2.0-0037466457en_HK
dc.identifier.hkuros87511en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037466457&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume304en_HK
dc.identifier.issue1en_HK
dc.identifier.spage60en_HK
dc.identifier.epage66en_HK
dc.identifier.isiWOS:000182589500011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK

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