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Article: Identification of novel genes expressed during spermatogenesis in stage-synchronized rat testes by differential display

TitleIdentification of novel genes expressed during spermatogenesis in stage-synchronized rat testes by differential display
Authors
KeywordsDifferential display
Spermatogenesis
Testis
Vitamin A deficiency
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2003, v. 307 n. 4, p. 782-790 How to Cite?
AbstractThe molecular mechanism regulating spermatogenesis at different developmental stages remains largely unknown. In a vitamin A-deficiency (VAD) rat model, five distinct histologically defined, stage-synchronized testes: (i) resting spermatogonia and preleptotene spermatocytes at Day 0 of post-vitamin A treatment (PVA); (ii) early pachytene spermatocytes at Day 7 PVA; (iii) late pachytene at Day 15 PVA; (iv) round spermatids at Day 25 PVA; and (v) elongated spermatids at Day 35 PVA were used to study gene expression profiles by mRNA differential display. Twenty-four differentially expressed cDNA fragments were identified and cloned; oligonucleotide sequence analyses indicated that there are 12 novel gene sequences, half of which share no apparent match in current GenBank/EMBL databases. Other 12 VAD clones share sequence homology to membrane channel and transport, transcription and translation, cell cycle and morphogenesis, inducer and transducer, surface or secreted glycoproteins or enzymes, and other miscellaneous molecules. Semi-quantitative RT-PCR analyses against different stages of VAD testes demonstrated: (i) restricted expression of VAD1.2 and 1.3 (novel) on Day 25 PVA when round spermatids form; (ii) escalating pattern of VAD12 (Cx43) in Sertoli cells; and (iii) relative constant levels of VAD4 (A5D3), VAD26.1 (ribonuclease), and VAD27 (GRP8) in spermatogenesis. © 2003 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87349
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorMok, BWen_HK
dc.contributor.authorShum, CKen_HK
dc.contributor.authorYeung, WSen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorTse, JYen_HK
dc.contributor.authorChow, JFen_HK
dc.contributor.authorWoo, Jen_HK
dc.contributor.authorKam, Ken_HK
dc.contributor.authorLee, KFen_HK
dc.date.accessioned2010-09-06T09:28:31Z-
dc.date.available2010-09-06T09:28:31Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2003, v. 307 n. 4, p. 782-790en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87349-
dc.description.abstractThe molecular mechanism regulating spermatogenesis at different developmental stages remains largely unknown. In a vitamin A-deficiency (VAD) rat model, five distinct histologically defined, stage-synchronized testes: (i) resting spermatogonia and preleptotene spermatocytes at Day 0 of post-vitamin A treatment (PVA); (ii) early pachytene spermatocytes at Day 7 PVA; (iii) late pachytene at Day 15 PVA; (iv) round spermatids at Day 25 PVA; and (v) elongated spermatids at Day 35 PVA were used to study gene expression profiles by mRNA differential display. Twenty-four differentially expressed cDNA fragments were identified and cloned; oligonucleotide sequence analyses indicated that there are 12 novel gene sequences, half of which share no apparent match in current GenBank/EMBL databases. Other 12 VAD clones share sequence homology to membrane channel and transport, transcription and translation, cell cycle and morphogenesis, inducer and transducer, surface or secreted glycoproteins or enzymes, and other miscellaneous molecules. Semi-quantitative RT-PCR analyses against different stages of VAD testes demonstrated: (i) restricted expression of VAD1.2 and 1.3 (novel) on Day 25 PVA when round spermatids form; (ii) escalating pattern of VAD12 (Cx43) in Sertoli cells; and (iii) relative constant levels of VAD4 (A5D3), VAD26.1 (ribonuclease), and VAD27 (GRP8) in spermatogenesis. © 2003 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectDifferential displayen_HK
dc.subjectSpermatogenesisen_HK
dc.subjectTestisen_HK
dc.subjectVitamin A deficiencyen_HK
dc.titleIdentification of novel genes expressed during spermatogenesis in stage-synchronized rat testes by differential displayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=307&issue=4&spage=782&epage=790&date=2003&atitle=Identification+of+novel+genes+expressed+during+spermatogenesis+in+stage-synchronized+rat+testes+by+differential+displayen_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailYeung, WS: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityYeung, WS=rp00331en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0006-291X(03)01250-6en_HK
dc.identifier.pmid12878178-
dc.identifier.scopuseid_2-s2.0-0038783231en_HK
dc.identifier.hkuros87579en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0038783231&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume307en_HK
dc.identifier.issue4en_HK
dc.identifier.spage782en_HK
dc.identifier.epage790en_HK
dc.identifier.isiWOS:000184510100005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridMok, BW=36786012900en_HK
dc.identifier.scopusauthoridShum, CK=35286215500en_HK
dc.identifier.scopusauthoridYeung, WS=7102370745en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridTse, JY=7102607314en_HK
dc.identifier.scopusauthoridChow, JF=7401728953en_HK
dc.identifier.scopusauthoridWoo, J=54889326800en_HK
dc.identifier.scopusauthoridKam, K=16426096200en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK

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