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Article: Preimplantation embryos cooperate with oviductal cells to produce embryotrophic inactivated complement-3b

TitlePreimplantation embryos cooperate with oviductal cells to produce embryotrophic inactivated complement-3b
Authors
Issue Date2008
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2008, v. 149 n. 3, p. 1268-1276 How to Cite?
AbstractHuman oviductal epithelial (OE) cells produce complement protein 3 (C3) and its derivatives, C3b and inactivated complement-3b (iC3b). Among them, iC3b is the most potent embryotrophic molecule. We studied the production of iC3b in the oviductal cell/embryo culture system. In the immune system, C3 convertase converts C3 into C3b, and the conversion of C3b to iC3b requires factor I (fI) and its cofactors, such as factor H or membrane cofactor protein. Human oviductal epithelium and OE cells expressed mRNA and protein of the components of C3 convertase, including C2, C4, factor B, and factor D. The OE cell-conditioned medium contained active C3 convertase activity that was suppressed by C3 convertase inhibitor, H17 in a dose and time-dependent manner. Although the oviductal epithelium and OE cells produced fI, the production of its cofactor, factor H required for the conversion of C3b to iC3b, was weak. Thus, OE cell-conditioned medium was inefficient in producing iC3b from exogenous C3b. On the contrary, mouse embryos facilitated such conversion to iC3b, which was taken up by the embryos, resulting in the formation of more blastocysts of larger size. The facilitatory activity was mediated by complement receptor 1-related gene/protein Y (Crry) with known membrane cofactor protein activity on the trophectoderm of the embryos as anti-Crry antibody inhibited the conversion and embryotrophic activity of C3b in the presence of fI. In conclusion, human oviduct possesses C3 convertase activity converting C3 to C3b, and Crry of the preimplantation embryos may be involved in the production of embryotrophic iC3b on the surface of the embryos. Copyright © 2008 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/87343
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTse, PKen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorChow, WNen_HK
dc.contributor.authorLuk, JMCen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:28:27Z-
dc.date.available2010-09-06T09:28:27Z-
dc.date.issued2008en_HK
dc.identifier.citationEndocrinology, 2008, v. 149 n. 3, p. 1268-1276en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87343-
dc.description.abstractHuman oviductal epithelial (OE) cells produce complement protein 3 (C3) and its derivatives, C3b and inactivated complement-3b (iC3b). Among them, iC3b is the most potent embryotrophic molecule. We studied the production of iC3b in the oviductal cell/embryo culture system. In the immune system, C3 convertase converts C3 into C3b, and the conversion of C3b to iC3b requires factor I (fI) and its cofactors, such as factor H or membrane cofactor protein. Human oviductal epithelium and OE cells expressed mRNA and protein of the components of C3 convertase, including C2, C4, factor B, and factor D. The OE cell-conditioned medium contained active C3 convertase activity that was suppressed by C3 convertase inhibitor, H17 in a dose and time-dependent manner. Although the oviductal epithelium and OE cells produced fI, the production of its cofactor, factor H required for the conversion of C3b to iC3b, was weak. Thus, OE cell-conditioned medium was inefficient in producing iC3b from exogenous C3b. On the contrary, mouse embryos facilitated such conversion to iC3b, which was taken up by the embryos, resulting in the formation of more blastocysts of larger size. The facilitatory activity was mediated by complement receptor 1-related gene/protein Y (Crry) with known membrane cofactor protein activity on the trophectoderm of the embryos as anti-Crry antibody inhibited the conversion and embryotrophic activity of C3b in the presence of fI. In conclusion, human oviduct possesses C3 convertase activity converting C3 to C3b, and Crry of the preimplantation embryos may be involved in the production of embryotrophic iC3b on the surface of the embryos. Copyright © 2008 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titlePreimplantation embryos cooperate with oviductal cells to produce embryotrophic inactivated complement-3ben_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=149&issue=3&spage=1268&epage=1276&date=2008&atitle=Preimplantation+embryos+cooperate+with+oviductal+cells+to+produce+embryotrophic+inactivated+complement-3ben_HK
dc.identifier.emailLee, YL: h9316321@hku.hken_HK
dc.identifier.emailLuk, JMC: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLuk, JMC=rp00349en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2007-1277en_HK
dc.identifier.pmid18039777-
dc.identifier.scopuseid_2-s2.0-40849140965en_HK
dc.identifier.hkuros154433en_HK
dc.identifier.hkuros158117-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-40849140965&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume149en_HK
dc.identifier.issue3en_HK
dc.identifier.spage1268en_HK
dc.identifier.epage1276en_HK
dc.identifier.isiWOS:000253421900050-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTse, PK=23978964600en_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridChow, WN=36852688700en_HK
dc.identifier.scopusauthoridLuk, JMC=7006777791en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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