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Article: Human oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos

TitleHuman oviductal cells reduce the incidence of apoptosis in cocultured mouse embryos
Authors
KeywordsApoptosis
Coculture
Embryo
Oviduct
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2000, v. 74 n. 6, p. 1215-1219 How to Cite?
AbstractObjective: To investigate the effect of human oviductal cell coculture on the incidence of apoptosis in mouse embryos. Design: Experimental laboratory study. Setting: University gynecology unit. Patient(s): Fallopian tubes were obtained from patients undergoing hysterectomy. Intervention(s): Mouse embryos were cocultured with human oviductal cells. Main Outcome Measure(s): Blastocyst development, allocation of inner cell mass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos. Results: Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0.005). This was due to increase in both the cell count of ICM (P<0.05) and TE (P<0.001). Coculture reduced the incidence of apoptosis in the cultured morula and blastocyst from 38% and 48% to 16% (P<0.001) and 27% (P<0.05), respectively. The number of apoptotic blastomeres per morula (1.5 ± 0.6; P<0.005) and blastocyst (2.3 ± 0.7; P<0.005) after coculture was also significantly lower than that of the corresponding control (morula, 2.1 ± 0.8; blastocyst, 3.5 ± 1.1). Conclusion(s): Human oviductal cells improved mouse embryo development partly by decreasing the incidence of apoptosis. Copyright (C) 2000 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87215
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Jen_HK
dc.contributor.authorCheung, TMen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:26:48Z-
dc.date.available2010-09-06T09:26:48Z-
dc.date.issued2000en_HK
dc.identifier.citationFertility And Sterility, 2000, v. 74 n. 6, p. 1215-1219en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87215-
dc.description.abstractObjective: To investigate the effect of human oviductal cell coculture on the incidence of apoptosis in mouse embryos. Design: Experimental laboratory study. Setting: University gynecology unit. Patient(s): Fallopian tubes were obtained from patients undergoing hysterectomy. Intervention(s): Mouse embryos were cocultured with human oviductal cells. Main Outcome Measure(s): Blastocyst development, allocation of inner cell mass (ICM) and trophectoderm (TE) in blastocyst, and apoptosis in embryos. Results: Oviductal cells significantly enhanced the blastulation (38%) and hatching rate (22%) of the cocultured zygotes. The corresponding values in medium alone culture were 21% and 9%, respectively. The cocultured embryos also had higher blastomere count at blastocyst stage (P<0.005). This was due to increase in both the cell count of ICM (P<0.05) and TE (P<0.001). Coculture reduced the incidence of apoptosis in the cultured morula and blastocyst from 38% and 48% to 16% (P<0.001) and 27% (P<0.05), respectively. The number of apoptotic blastomeres per morula (1.5 ± 0.6; P<0.005) and blastocyst (2.3 ± 0.7; P<0.005) after coculture was also significantly lower than that of the corresponding control (morula, 2.1 ± 0.8; blastocyst, 3.5 ± 1.1). Conclusion(s): Human oviductal cells improved mouse embryo development partly by decreasing the incidence of apoptosis. Copyright (C) 2000 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectApoptosisen_HK
dc.subjectCocultureen_HK
dc.subjectEmbryoen_HK
dc.subjectOviducten_HK
dc.titleHuman oviductal cells reduce the incidence of apoptosis in cocultured mouse embryosen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=74&issue=6&spage=1215&epage=1219&date=2000&atitle=Human+oviductal+cells+reduce+the+incidence+of+apoptosis+in+cocultured+mouse+embryosen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(00)01618-6en_HK
dc.identifier.pmid11119753-
dc.identifier.scopuseid_2-s2.0-0033637018en_HK
dc.identifier.hkuros56311en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033637018&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume74en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1215en_HK
dc.identifier.epage1219en_HK
dc.identifier.isiWOS:000165897300026-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, J=7408556691en_HK
dc.identifier.scopusauthoridCheung, TM=7103334551en_HK
dc.identifier.scopusauthoridChan, STH=36901169900en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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