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Article: Human oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro

TitleHuman oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro
Authors
KeywordsMotility
Oviductal cells
Spermatozoa
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2000, v. 73 n. 3, p. 479-486 How to Cite?
AbstractObjective: To characterize in part the factor(s) in conditioned medium (CM) that maintains sperm motility after human oviductal cell culture. Design: Controlled, experimental, laboratory study. Setting: University-based gynecology unit. Patient(s): Fallopian tubes were obtained from patients who underwent tubal ligation or hysterectomy. Semen with normal sperm parameters was obtained from men who visited subfertility clinics. Intervention(s): Spermatozoa were incubated with CM and their motility was evaluated by a computer-aided sperm analysis system. Main Outcome Measure(s): Curvilinear velocity, straight-line velocity, average path velocity, linearity, amplitude of lateral head displacement, beat cross-frequency, and percentage of spermatozoa that exhibited hyperactivation. Result(s): Compared with their baseline motility (0 hour), spermatozoa incubated with CM maintained various motility parameters for a longer period than did control spermatozoa. All the motility parameters of the CM-treated spermatozoa were higher than those of the control spermatozoa at the same time point. This effect of CM was dose- dependent and increased with the duration of incubation. The effect was stable at 56°C but was not observed after 100°C heat treatment. Trypsin, but not proteinase K, abolished the effect. A fraction with a molecular weight of <3 kd in the CM was responsible for the observed effect. Conclusion(s): Human oviductal cells produce a peptide(s) that maintains sperm motility. (C)2000 by American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87185
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:26:25Z-
dc.date.available2010-09-06T09:26:25Z-
dc.date.issued2000en_HK
dc.identifier.citationFertility And Sterility, 2000, v. 73 n. 3, p. 479-486en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87185-
dc.description.abstractObjective: To characterize in part the factor(s) in conditioned medium (CM) that maintains sperm motility after human oviductal cell culture. Design: Controlled, experimental, laboratory study. Setting: University-based gynecology unit. Patient(s): Fallopian tubes were obtained from patients who underwent tubal ligation or hysterectomy. Semen with normal sperm parameters was obtained from men who visited subfertility clinics. Intervention(s): Spermatozoa were incubated with CM and their motility was evaluated by a computer-aided sperm analysis system. Main Outcome Measure(s): Curvilinear velocity, straight-line velocity, average path velocity, linearity, amplitude of lateral head displacement, beat cross-frequency, and percentage of spermatozoa that exhibited hyperactivation. Result(s): Compared with their baseline motility (0 hour), spermatozoa incubated with CM maintained various motility parameters for a longer period than did control spermatozoa. All the motility parameters of the CM-treated spermatozoa were higher than those of the control spermatozoa at the same time point. This effect of CM was dose- dependent and increased with the duration of incubation. The effect was stable at 56°C but was not observed after 100°C heat treatment. Trypsin, but not proteinase K, abolished the effect. A fraction with a molecular weight of <3 kd in the CM was responsible for the observed effect. Conclusion(s): Human oviductal cells produce a peptide(s) that maintains sperm motility. (C)2000 by American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectMotilityen_HK
dc.subjectOviductal cellsen_HK
dc.subjectSpermatozoaen_HK
dc.titleHuman oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=73&issue=3&spage=479&epage=486&date=2000&atitle=Human+oviductal+cells+produce+a+factor(s)+that+maintains+the+motility+of+human+spermatozoa+in+vitroen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(99)00563-4en_HK
dc.identifier.pmid10688999-
dc.identifier.scopuseid_2-s2.0-0033977123en_HK
dc.identifier.hkuros48903en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033977123&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue3en_HK
dc.identifier.spage479en_HK
dc.identifier.epage486en_HK
dc.identifier.isiWOS:000085445800008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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