Human oviductal cells produce a factor(s) that maintains the motility of human spermatozoa in vitro

Abstract

Objective: To characterize in part the factor(s) in conditioned medium (CM) that maintains sperm motility after human oviductal cell culture. Design: Controlled, experimental, laboratory study. Setting: University-based gynecology unit. Patient(s): Fallopian tubes were obtained from patients who underwent tubal ligation or hysterectomy. Semen with normal sperm parameters was obtained from men who visited subfertility clinics. Intervention(s): Spermatozoa were incubated with CM and their motility was evaluated by a computer-aided sperm analysis system. Main Outcome Measure(s): Curvilinear velocity, straight-line velocity, average path velocity, linearity, amplitude of lateral head displacement, beat cross-frequency, and percentage of spermatozoa that exhibited hyperactivation. Result(s): Compared with their baseline motility (0 hour), spermatozoa incubated with CM maintained various motility parameters for a longer period than did control spermatozoa. All the motility parameters of the CM-treated spermatozoa were higher than those of the control spermatozoa at the same time point. This effect of CM was dose- dependent and increased with the duration of incubation. The effect was stable at 56°C but was not observed after 100°C heat treatment. Trypsin, but not proteinase K, abolished the effect. A fraction with a molecular weight of <3 kd in the CM was responsible for the observed effect. Conclusion(s): Human oviductal cells produce a peptide(s) that maintains sperm motility. (C)2000 by American Society for Reproductive Medicine.