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Article: Low mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytes

TitleLow mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytes
Authors
KeywordsEmbryo development
Mitochondria
Oocyte
PolScope
Spindle
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2007, v. 22 n. 6, p. 1681-1686 How to Cite?
AbstractBackground: Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated the relationship between birefrigent meiotic spindle and cytoplasmic mitochondrial DNA (mtDNA) and ATP contents in in vitro matured human oocytes. Methods: Oocytes at germinal vesicle stage were collected and cultured for 24-48 h with or without the metabolic inhibitor, carbonyl cyanide p-(tri-fluromethoxy) phenyl-hydrazone (FCCP). All in vitro matured oocytes were examined by PolScope for the presence of meiotic spindle, then the oocytes were used for either intracytoplasmic sperm injection or the measurement of mitochondrial quantity and ATP content. Results: Meiotic spindles were observed in 51.3% (60/117) of the in vitro matured oocytes. Oocytes with detectable meiotic spindle contained significantly higher mtDNA copies (637 250 ± 237 606 versus 491 454 ± 153 406, P = 0.027) and ATP content (1.97 ± 0.38 versus 1.65 ± 0.32 pmol, P = 0.028) when compared with those without detectable meiotic spindle. However, in vitro matured oocytes showed a significantly reduced rate of positive meiotic spindle and a lower ATP content when cultured with FCCP. A lower incidence of normal fertilization and good quality embryos were observed if meiotic spindles were not detected. Conclusions: Low mtDNA and ATP content might contribute to the absence of birefringent spindle imaged with the PolScope in human in vitro matured oocytes. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87172
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZeng, HTen_HK
dc.contributor.authorRen, Zen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorShu, YMen_HK
dc.contributor.authorXu, YWen_HK
dc.contributor.authorZhuang, GLen_HK
dc.contributor.authorLiang, XYen_HK
dc.date.accessioned2010-09-06T09:26:15Z-
dc.date.available2010-09-06T09:26:15Z-
dc.date.issued2007en_HK
dc.identifier.citationHuman Reproduction, 2007, v. 22 n. 6, p. 1681-1686en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87172-
dc.description.abstractBackground: Birefrigent meiotic spindle in live human oocytes can be visualized by the PolScope. This study investigated the relationship between birefrigent meiotic spindle and cytoplasmic mitochondrial DNA (mtDNA) and ATP contents in in vitro matured human oocytes. Methods: Oocytes at germinal vesicle stage were collected and cultured for 24-48 h with or without the metabolic inhibitor, carbonyl cyanide p-(tri-fluromethoxy) phenyl-hydrazone (FCCP). All in vitro matured oocytes were examined by PolScope for the presence of meiotic spindle, then the oocytes were used for either intracytoplasmic sperm injection or the measurement of mitochondrial quantity and ATP content. Results: Meiotic spindles were observed in 51.3% (60/117) of the in vitro matured oocytes. Oocytes with detectable meiotic spindle contained significantly higher mtDNA copies (637 250 ± 237 606 versus 491 454 ± 153 406, P = 0.027) and ATP content (1.97 ± 0.38 versus 1.65 ± 0.32 pmol, P = 0.028) when compared with those without detectable meiotic spindle. However, in vitro matured oocytes showed a significantly reduced rate of positive meiotic spindle and a lower ATP content when cultured with FCCP. A lower incidence of normal fertilization and good quality embryos were observed if meiotic spindles were not detected. Conclusions: Low mtDNA and ATP content might contribute to the absence of birefringent spindle imaged with the PolScope in human in vitro matured oocytes. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectEmbryo developmenten_HK
dc.subjectMitochondriaen_HK
dc.subjectOocyteen_HK
dc.subjectPolScopeen_HK
dc.subjectSpindleen_HK
dc.titleLow mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=22&issue=6&spage=1681&epage=1686&date=2007&atitle=Low+mitochondrial+DNA+and+ATP+contents+contribute+to+the+absence+of+birefringent+spindle+imaged+with+PolScope+in+in+vitro+matured+human+oocytesen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/humrep/dem070en_HK
dc.identifier.pmid17449512-
dc.identifier.scopuseid_2-s2.0-34447321569en_HK
dc.identifier.hkuros131312en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447321569&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1681en_HK
dc.identifier.epage1686en_HK
dc.identifier.isiWOS:000247470700028-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZeng, HT=16837514100en_HK
dc.identifier.scopusauthoridRen, Z=7402408519en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridShu, YM=7103239434en_HK
dc.identifier.scopusauthoridXu, YW=8450843700en_HK
dc.identifier.scopusauthoridZhuang, GL=7004628287en_HK
dc.identifier.scopusauthoridLiang, XY=7401735965en_HK

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