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Article: Noncompetitive immunoassays using protein G affinity capillary chromatography and capillary electrophoresis with laser-induced fluorescence detection

TitleNoncompetitive immunoassays using protein G affinity capillary chromatography and capillary electrophoresis with laser-induced fluorescence detection
Authors
KeywordsImmunoassays
Proteins
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chroma
Citation
Journal Of Chromatography A, 1999, v. 848 n. 1-2, p. 139-148 How to Cite?
AbstractA new and simple approach to perform immunoassay using protein G affinity capillary chromatography and laser-induced fluorescence detection was described. A noncompetitive assay for monoclonal anti-bovine serum albumin (BSA) was used to test the performance of the system. Fluorescein isothiocyanate labeled BSA was used as a tracer to determine anti-BSA in pM level. Capillaries with inner diameter of 150 μm were packed with recombinant protein G-bound perfusive support. The packed capillary was used to capture the immunocomplexes, which were subsequently desorbed by 100 mM glycine (pH 9.0). Open tube capillary electrophoresis-based immunoassay (CEIA) for anti-BSA was also performed. Using standard samples, calibration curves for anti-BSA was established in both assays. Compared with CEIA, this system improved the concentration sensitivity for about 100-fold due to the pre-concentration of immunocomplex in the protein G column, while the mass sensitivity was similar in the two methods. Copyright (C) 1999 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/87155
ISSN
2015 Impact Factor: 3.926
2015 SCImago Journal Rankings: 1.774
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Qen_HK
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorOu, Jen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:26:02Z-
dc.date.available2010-09-06T09:26:02Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Chromatography A, 1999, v. 848 n. 1-2, p. 139-148en_HK
dc.identifier.issn0021-9673en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87155-
dc.description.abstractA new and simple approach to perform immunoassay using protein G affinity capillary chromatography and laser-induced fluorescence detection was described. A noncompetitive assay for monoclonal anti-bovine serum albumin (BSA) was used to test the performance of the system. Fluorescein isothiocyanate labeled BSA was used as a tracer to determine anti-BSA in pM level. Capillaries with inner diameter of 150 μm were packed with recombinant protein G-bound perfusive support. The packed capillary was used to capture the immunocomplexes, which were subsequently desorbed by 100 mM glycine (pH 9.0). Open tube capillary electrophoresis-based immunoassay (CEIA) for anti-BSA was also performed. Using standard samples, calibration curves for anti-BSA was established in both assays. Compared with CEIA, this system improved the concentration sensitivity for about 100-fold due to the pre-concentration of immunocomplex in the protein G column, while the mass sensitivity was similar in the two methods. Copyright (C) 1999 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/chromaen_HK
dc.relation.ispartofJournal of Chromatography Aen_HK
dc.rightsJournal of Chromatography A. Copyright © Elsevier BV.en_HK
dc.subjectImmunoassaysen_HK
dc.subjectProteinsen_HK
dc.titleNoncompetitive immunoassays using protein G affinity capillary chromatography and capillary electrophoresis with laser-induced fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9673&volume=848&issue=1-2&spage=139&epage=148&date=1999&atitle=Noncompetitive+immunoassays+using+protein+G+affinity+capillary+chromatography+and+capillary+electrophoresis+with+laser+induced+fluorescence+detectionen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0021-9673(99)00413-6en_HK
dc.identifier.pmid10427754en_HK
dc.identifier.scopuseid_2-s2.0-0033056401en_HK
dc.identifier.hkuros42300en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033056401&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume848en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage139en_HK
dc.identifier.epage148en_HK
dc.identifier.isiWOS:000081413600013-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridWang, Q=7406915351en_HK
dc.identifier.scopusauthoridLuo, G=7401536462en_HK
dc.identifier.scopusauthoridOu, J=7202845297en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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