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Article: The incidence of cytoplasmic fragmentation in mouse embryos in vitro is not affected by inhibition of caspase activity

TitleThe incidence of cytoplasmic fragmentation in mouse embryos in vitro is not affected by inhibition of caspase activity
Authors
KeywordsApoptosis
Caspases
Embryo
Fragmentation
Issue Date2001
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2001, v. 75 n. 5, p. 986-991 How to Cite?
AbstractObjective: To investigate the relationship between cytoplasmic fragmentation and caspase activity in the mouse embryo. Design: Experimental laboratory study. Setting: University gynacology unit. Animal(s): One-cell zygote of mouse (MF1 × BALB/c). Intervention(s): Mouse embryos were treated with caspase inhibitors: benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone (z-VAD-fmk) and benzyloxycarbonyl-Asp-glu-Val-Asp-fluoromethyl ketone (Z-DEVD-fmk). Main Outcome Measure(s): Morphological development of the embryo, proportion of fragmented embryos, caspase-3-like activity, DNA breakage, and phosphatidylserine exposure in blastomeres. Result(s): The proportion of embryo reaching two-cell, three- to four-cell, and morula stage at 48, 72, and 96 hours after hCG administration, respectively, were comparable between the control embryos and those treated with either z-VAD-fmk or z-DEVD-fmk, at three concentrations (10 μM, 50 μM, and 200 μM). Although the inhibitors suppressed the caspase-3-like activity in the embryo fragment before compaction and decreased DNA breakages, there was no statistically significant difference in the percentage of fragmented embryo between the control and those treated with caspase inhibitors. The inhibitors did not affect the incidence of phosphatidylserine exposure in the blastomere of the treated embryos. Conclusion(s): Cytoplasmic fragmentation in precompaction mouse embryos is not a consequence of caspase-related apoptosis. Copyright © 2001 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87122
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, JSen_HK
dc.contributor.authorCheung, TMen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:25:36Z-
dc.date.available2010-09-06T09:25:36Z-
dc.date.issued2001en_HK
dc.identifier.citationFertility And Sterility, 2001, v. 75 n. 5, p. 986-991en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87122-
dc.description.abstractObjective: To investigate the relationship between cytoplasmic fragmentation and caspase activity in the mouse embryo. Design: Experimental laboratory study. Setting: University gynacology unit. Animal(s): One-cell zygote of mouse (MF1 × BALB/c). Intervention(s): Mouse embryos were treated with caspase inhibitors: benzyloxycarbonyl-Val-Ala-Asp fluoromethylketone (z-VAD-fmk) and benzyloxycarbonyl-Asp-glu-Val-Asp-fluoromethyl ketone (Z-DEVD-fmk). Main Outcome Measure(s): Morphological development of the embryo, proportion of fragmented embryos, caspase-3-like activity, DNA breakage, and phosphatidylserine exposure in blastomeres. Result(s): The proportion of embryo reaching two-cell, three- to four-cell, and morula stage at 48, 72, and 96 hours after hCG administration, respectively, were comparable between the control embryos and those treated with either z-VAD-fmk or z-DEVD-fmk, at three concentrations (10 μM, 50 μM, and 200 μM). Although the inhibitors suppressed the caspase-3-like activity in the embryo fragment before compaction and decreased DNA breakages, there was no statistically significant difference in the percentage of fragmented embryo between the control and those treated with caspase inhibitors. The inhibitors did not affect the incidence of phosphatidylserine exposure in the blastomere of the treated embryos. Conclusion(s): Cytoplasmic fragmentation in precompaction mouse embryos is not a consequence of caspase-related apoptosis. Copyright © 2001 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectApoptosisen_HK
dc.subjectCaspasesen_HK
dc.subjectEmbryoen_HK
dc.subjectFragmentationen_HK
dc.titleThe incidence of cytoplasmic fragmentation in mouse embryos in vitro is not affected by inhibition of caspase activityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=75&issue=5&spage=986&epage=991&date=2001&atitle=The+incidence+of+cytoplasmic+fragmentation+in+mouse+embryos+in+vitro+is+not+affected+by+inhibition+of+caspase+activityen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(01)01687-9en_HK
dc.identifier.pmid11334913-
dc.identifier.scopuseid_2-s2.0-0035028713en_HK
dc.identifier.hkuros65581en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035028713&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue5en_HK
dc.identifier.spage986en_HK
dc.identifier.epage991en_HK
dc.identifier.isiWOS:000168654500022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridCheung, TM=7103334551en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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