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Article: Vero cells, but not oviductal cells, increase the hatching frequency and total cell count of mouse blastocysts partly by changing energy substrate concentrations in culture medium

TitleVero cells, but not oviductal cells, increase the hatching frequency and total cell count of mouse blastocysts partly by changing energy substrate concentrations in culture medium
Authors
KeywordsCoculture
Embryo
Glutamine
Lactate
Pyruvate
Issue Date2001
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1058-0468
Citation
Journal Of Assisted Reproduction And Genetics, 2001, v. 18 n. 10, p. 566-574 How to Cite?
AbstractPurpose: To investigate the embryotrophic mechanisms of Vero and oviductal cells coculture. Methods: Mouse embryos were cultured in Chatot, Ziomek, and Bavister medium (CZB), in modified CZB media (MM) with nutrient concentrations adjusted to that found in conditioned media after different periods of Vero cells or oviductal cells culture, in reconstituted medium (RM) containing the purified > 100-kDa components of Vero cell conditioned medium that had been reconstituted with CZB medium, and cocultured with Vero cells with an interposing membrane. Results: The blastulation rate was not different among embryos cultured in different Vero-cell-derived MMs. Nine-hour Vero-cell-derived MM significantly increased the total cell number and hatching frequency of the embryos. There was no difference in these parameters with oviductal-cell-derived MMs. The RM of Vero cells did not possess embryotrophic activity. The presence of a porous membrane between Vero cells and embryos did not affect the embryotrophic activity of coculture. Conclusions: Vero cells, but not oviductal cells, improved mouse embryo development partly by modifying the energy substrate concentration in culture medium.
Persistent Identifierhttp://hdl.handle.net/10722/87108
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 0.858
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, YLen_HK
dc.contributor.authorXu, JSen_HK
dc.contributor.authorChan, STHen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:25:25Z-
dc.date.available2010-09-06T09:25:25Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Assisted Reproduction And Genetics, 2001, v. 18 n. 10, p. 566-574en_HK
dc.identifier.issn1058-0468en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87108-
dc.description.abstractPurpose: To investigate the embryotrophic mechanisms of Vero and oviductal cells coculture. Methods: Mouse embryos were cultured in Chatot, Ziomek, and Bavister medium (CZB), in modified CZB media (MM) with nutrient concentrations adjusted to that found in conditioned media after different periods of Vero cells or oviductal cells culture, in reconstituted medium (RM) containing the purified > 100-kDa components of Vero cell conditioned medium that had been reconstituted with CZB medium, and cocultured with Vero cells with an interposing membrane. Results: The blastulation rate was not different among embryos cultured in different Vero-cell-derived MMs. Nine-hour Vero-cell-derived MM significantly increased the total cell number and hatching frequency of the embryos. There was no difference in these parameters with oviductal-cell-derived MMs. The RM of Vero cells did not possess embryotrophic activity. The presence of a porous membrane between Vero cells and embryos did not affect the embryotrophic activity of coculture. Conclusions: Vero cells, but not oviductal cells, improved mouse embryo development partly by modifying the energy substrate concentration in culture medium.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=1058-0468en_HK
dc.relation.ispartofJournal of Assisted Reproduction and Geneticsen_HK
dc.subjectCocultureen_HK
dc.subjectEmbryoen_HK
dc.subjectGlutamineen_HK
dc.subjectLactateen_HK
dc.subjectPyruvateen_HK
dc.titleVero cells, but not oviductal cells, increase the hatching frequency and total cell count of mouse blastocysts partly by changing energy substrate concentrations in culture mediumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1058-0468&volume=18&spage=566&epage=574&date=2001&atitle=Vero+Cells,+but+not+oviductal+cells,+increase+the+hatching+frequency+and+total+cell+count+of+mouse+blastocysts+partly+by+changing+energy+substrate+concentrations+in+culture+medium.en_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1011910125079en_HK
dc.identifier.pmid11699129-
dc.identifier.scopuseid_2-s2.0-0034757251en_HK
dc.identifier.hkuros112500en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034757251&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue10en_HK
dc.identifier.spage566en_HK
dc.identifier.epage574en_HK
dc.identifier.isiWOS:000171815600008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridXu, JS=7408556691en_HK
dc.identifier.scopusauthoridChan, STH=24368283200en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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