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Article: Capillary electrophoresis based immunoassay for monoclonal antibody with diode laser induced fluorescence detection

TitleCapillary electrophoresis based immunoassay for monoclonal antibody with diode laser induced fluorescence detection
Authors
KeywordsCapillary electrophoresis
Diode laser induced fluorescence detection
Immunoassay
Monoclonal antibody
Issue Date2000
PublisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00032719.asp
Citation
Analytical Letters, 2000, v. 33 n. 4, p. 589-602 How to Cite?
AbstractA capillary electrophoresis based immunoassay (CEIA) for monoclonal antibody using diode laser induced fluorescence (LIF) detection was described. A direct assay for monoclonal anti-BSA in mouse serum was used as a model. BSA was labeled with Cy5 and used as the immunoreagent. The 635 nm line of a diode laser was used as the excitation source for LIF detection. The calibration curve for anti-BSA in mouse serum had a linear dynamic range of 4-40 nM. The concentration limit of detection (LOD) was 1.2 nM. Incubation time and CE conditions such as buffer concentration, pH and separation voltage were optimized, and the performances of different lasers as excitation sources were also compared.
Persistent Identifierhttp://hdl.handle.net/10722/87106
ISSN
2015 Impact Factor: 1.088
2015 SCImago Journal Rankings: 0.368
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Qen_HK
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:25:24Z-
dc.date.available2010-09-06T09:25:24Z-
dc.date.issued2000en_HK
dc.identifier.citationAnalytical Letters, 2000, v. 33 n. 4, p. 589-602en_HK
dc.identifier.issn0003-2719en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87106-
dc.description.abstractA capillary electrophoresis based immunoassay (CEIA) for monoclonal antibody using diode laser induced fluorescence (LIF) detection was described. A direct assay for monoclonal anti-BSA in mouse serum was used as a model. BSA was labeled with Cy5 and used as the immunoreagent. The 635 nm line of a diode laser was used as the excitation source for LIF detection. The calibration curve for anti-BSA in mouse serum had a linear dynamic range of 4-40 nM. The concentration limit of detection (LOD) was 1.2 nM. Incubation time and CE conditions such as buffer concentration, pH and separation voltage were optimized, and the performances of different lasers as excitation sources were also compared.en_HK
dc.languageengen_HK
dc.publisherTaylor & Francis Inc. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00032719.aspen_HK
dc.relation.ispartofAnalytical Lettersen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectDiode laser induced fluorescence detectionen_HK
dc.subjectImmunoassayen_HK
dc.subjectMonoclonal antibodyen_HK
dc.titleCapillary electrophoresis based immunoassay for monoclonal antibody with diode laser induced fluorescence detectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-2719&volume=33&issue=4&spage=589&epage=602&date=1999&atitle=Capillary+electrophoresis+based+immunoassay+for+monoclonal+antibody+with+diode+laser+induced+fluorescence+detectionen_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/00032710008543076-
dc.identifier.scopuseid_2-s2.0-0034064113en_HK
dc.identifier.hkuros59251en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034064113&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume33en_HK
dc.identifier.issue4en_HK
dc.identifier.spage589en_HK
dc.identifier.epage602en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, Q=7406915351en_HK
dc.identifier.scopusauthoridLuo, G=7401536462en_HK
dc.identifier.scopusauthoridWang, Y=13310049900en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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