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Article: Differential expression of gap-junction gene connexin 31 in seminiferous epithelium of rat testes

TitleDifferential expression of gap-junction gene connexin 31 in seminiferous epithelium of rat testes
Authors
KeywordsConnexin 31
Gap junction
Germ cell
Seminiferous epithelium
Spermatogenesis
Issue Date1999
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febslet
Citation
Febs Letters, 1999, v. 453 n. 3, p. 243-248 How to Cite?
AbstractSpermatogenesis, a tightly regulated developmental process of male germ cells in testis, is associated with temporal and spatial expression of certain gap-junction connexins. Our findings by RT-PCR indicate that the Cx31 gene is expressed in testis tissue of adult and postnatal rats. During the postnatal spermatogenic process, the Cx31-specific signal became detectable at 15 dpp and onward by in situ hybridization, and apparently localized in the basal compartment of seminiferous epithelium where active spermatogonia and early primary spermatocytes reside. No signal was found in the luminal region. In adult testes, spermatids of elongation phase were also Cx31 positive. Immunohistochemical analysis with mouse anti-Cx31 antibody gave a similar staining pattern, providing further evidence that the gap-junction protein is abundant in the basal seminiferous epithelium, in accordance with the cellular distribution of Cx31 mRNA. These results represent the first demonstration of Cx31 expression at both transcriptional and protein level in the seminiferous epithelium of rat testes. Thus, Cx31 may play a role in cell-cell communication during spermatogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/87093
ISSN
2015 Impact Factor: 3.519
2015 SCImago Journal Rankings: 2.026
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMok, BWen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLuk, JMen_HK
dc.date.accessioned2010-09-06T09:25:14Z-
dc.date.available2010-09-06T09:25:14Z-
dc.date.issued1999en_HK
dc.identifier.citationFebs Letters, 1999, v. 453 n. 3, p. 243-248en_HK
dc.identifier.issn0014-5793en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87093-
dc.description.abstractSpermatogenesis, a tightly regulated developmental process of male germ cells in testis, is associated with temporal and spatial expression of certain gap-junction connexins. Our findings by RT-PCR indicate that the Cx31 gene is expressed in testis tissue of adult and postnatal rats. During the postnatal spermatogenic process, the Cx31-specific signal became detectable at 15 dpp and onward by in situ hybridization, and apparently localized in the basal compartment of seminiferous epithelium where active spermatogonia and early primary spermatocytes reside. No signal was found in the luminal region. In adult testes, spermatids of elongation phase were also Cx31 positive. Immunohistochemical analysis with mouse anti-Cx31 antibody gave a similar staining pattern, providing further evidence that the gap-junction protein is abundant in the basal seminiferous epithelium, in accordance with the cellular distribution of Cx31 mRNA. These results represent the first demonstration of Cx31 expression at both transcriptional and protein level in the seminiferous epithelium of rat testes. Thus, Cx31 may play a role in cell-cell communication during spermatogenesis.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/febsleten_HK
dc.relation.ispartofFEBS Lettersen_HK
dc.rightsF E B S Letters. Copyright © Elsevier BV.en_HK
dc.subjectConnexin 31en_HK
dc.subjectGap junctionen_HK
dc.subjectGerm cellen_HK
dc.subjectSeminiferous epitheliumen_HK
dc.subjectSpermatogenesisen_HK
dc.titleDifferential expression of gap-junction gene connexin 31 in seminiferous epithelium of rat testesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0014-5793&volume=452&spage=243&epage=248&date=1999&atitle=Differential+expression+of+gap-junction+gene+connexin+31+in+seminiferous+epithelium+of+rat+testesen_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0014-5793(99)00714-0en_HK
dc.identifier.pmid10405153-
dc.identifier.scopuseid_2-s2.0-0032812569en_HK
dc.identifier.hkuros41587en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032812569&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume453en_HK
dc.identifier.issue3en_HK
dc.identifier.spage243en_HK
dc.identifier.epage248en_HK
dc.identifier.isiWOS:000081242100002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridMok, BW=36786012900en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK

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