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Article: Establishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorus

TitleEstablishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorus
Authors
KeywordsCumulus oophorus
Human spermatozoa
Issue Date2004
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2004, v. 19 n. 7, p. 1562-1569 How to Cite?
AbstractBackground: Spermatozoa have to traverse the cumulus oophorus before fertilization in vivo. Evidence suggests that the cumulus oophorus plays an important role in the fertilization process. We describe the establishment of a capillary-cumulus oophorus model with which to study the action of cumulus mass on the function of human spermatozoa. Methods: Human cumulus oophorus was aspirated into a glass capillary. Spermatozoa were allowed to pass through the cumulus mass in the capillary from one end of the capillary. The spermatozoa that had traversed the mass were collected at the other end of the capillary and underwent sperm function analyses. Results: Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to have normal morphology, be capacitated and acrosome reacted, with a distinct motility pattern and better zona-binding capacity. Conclusion: A novel in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions. © European Society of Human Reproduction and Embryology 2004; all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87069
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHong, SJen_HK
dc.contributor.authorChiu, PCen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorTse, JMYen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:24:55Z-
dc.date.available2010-09-06T09:24:55Z-
dc.date.issued2004en_HK
dc.identifier.citationHuman Reproduction, 2004, v. 19 n. 7, p. 1562-1569en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87069-
dc.description.abstractBackground: Spermatozoa have to traverse the cumulus oophorus before fertilization in vivo. Evidence suggests that the cumulus oophorus plays an important role in the fertilization process. We describe the establishment of a capillary-cumulus oophorus model with which to study the action of cumulus mass on the function of human spermatozoa. Methods: Human cumulus oophorus was aspirated into a glass capillary. Spermatozoa were allowed to pass through the cumulus mass in the capillary from one end of the capillary. The spermatozoa that had traversed the mass were collected at the other end of the capillary and underwent sperm function analyses. Results: Compared with those spermatozoa cultured in medium alone, spermatozoa exposed to the cumulus mass were more likely to have normal morphology, be capacitated and acrosome reacted, with a distinct motility pattern and better zona-binding capacity. Conclusion: A novel in vitro model for spermatozoa penetration through the cumulus oophorus was established. The model can be applied to investigate the effect of the cumulus oophorus on sperm functions. © European Society of Human Reproduction and Embryology 2004; all rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectCumulus oophorusen_HK
dc.subjectHuman spermatozoaen_HK
dc.titleEstablishment of a capillary-cumulus model to study the selection of sperm for fertilization by the cumulus oophorusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=19&issue=7&spage=1562&epage=1569&date=2004&atitle=Establishment+of+a+capillary-cumulus+model+to+study+the+selection+of+sperm+for+fertilization+by+the+cumulus+oophorusen_HK
dc.identifier.emailChiu, PC:pchiucn@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PC=rp00424en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/humrep/deh281en_HK
dc.identifier.pmid15178655-
dc.identifier.scopuseid_2-s2.0-3242879732en_HK
dc.identifier.hkuros89196en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3242879732&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1562en_HK
dc.identifier.epage1569en_HK
dc.identifier.isiWOS:000222400100013-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHong, SJ=7405767201en_HK
dc.identifier.scopusauthoridChiu, PC=25959969200en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridTse, JMY=8050376700en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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