File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effects of human follicular fluid on the capacitation and motility of human spermatozoa

TitleEffects of human follicular fluid on the capacitation and motility of human spermatozoa
Authors
KeywordsAcrosome reaction
Capacitation
Human follicular fluid
Motility
Spermatozoa
Issue Date2000
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2000, v. 73 n. 4, p. 680-686 How to Cite?
AbstractObjective: To investigate the capacitation and motility kinetics of spermatozoa treated with human follicular fluid (FF). Design: Controlled, experimental laboratory study.Setting: University-based gynecology unit. Patient(s): Human FF was collected from women undergoing assisted reproductive treatment. Semen samples were obtained from men visiting subfertility clinics. Intervention(s): Spermatozoa were incubated with human FF under various experimental conditions. Spermatozoa incubated with Earle's balanced salt solution were used as the control. Main Outcome Measure(s): Chlortetracycline staining patterns and sperm motility parameters. Result(s): The rate of capacitation in the human FF-treated spermatozoa was significantly higher than that in the control spermatozoa after 1 hour and 3 hours of treatment. The percentage of acrosome-reacted spermatozoa also was significantly higher after human FF treatment than after control treatment. These effects of human FF were dose-dependent. Human FF-treated spermatozoa maintained their velocities at the zero-hour level for 5 hours, whereas the velocities of the control spermatozoa decreased significantly after 1 hour. Human FF treatment significantly increased the beat cross-frequency above the rate at zero hour for 5 hours. The hyperactivation of the human FF-treated spermatozoa remained stable for 3 hours, whereas that of the control spermatozoa decreased significantly after 1 hour of incubation. Significantly more human FF-treated spermatozoa underwent hyperactivation than did control spermatozoa after 1 hour and 3 hours of treatment. The effects of human FF on beat cross-frequency and hyperactivation were dose-dependent. Conclusion(s): Human FF promotes capacitation and the acrosome reaction within a short period. It also stimulates or maintains various sperm motility parameters. Copyright (C) 2000 American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87051
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:24:41Z-
dc.date.available2010-09-06T09:24:41Z-
dc.date.issued2000en_HK
dc.identifier.citationFertility And Sterility, 2000, v. 73 n. 4, p. 680-686en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87051-
dc.description.abstractObjective: To investigate the capacitation and motility kinetics of spermatozoa treated with human follicular fluid (FF). Design: Controlled, experimental laboratory study.Setting: University-based gynecology unit. Patient(s): Human FF was collected from women undergoing assisted reproductive treatment. Semen samples were obtained from men visiting subfertility clinics. Intervention(s): Spermatozoa were incubated with human FF under various experimental conditions. Spermatozoa incubated with Earle's balanced salt solution were used as the control. Main Outcome Measure(s): Chlortetracycline staining patterns and sperm motility parameters. Result(s): The rate of capacitation in the human FF-treated spermatozoa was significantly higher than that in the control spermatozoa after 1 hour and 3 hours of treatment. The percentage of acrosome-reacted spermatozoa also was significantly higher after human FF treatment than after control treatment. These effects of human FF were dose-dependent. Human FF-treated spermatozoa maintained their velocities at the zero-hour level for 5 hours, whereas the velocities of the control spermatozoa decreased significantly after 1 hour. Human FF treatment significantly increased the beat cross-frequency above the rate at zero hour for 5 hours. The hyperactivation of the human FF-treated spermatozoa remained stable for 3 hours, whereas that of the control spermatozoa decreased significantly after 1 hour of incubation. Significantly more human FF-treated spermatozoa underwent hyperactivation than did control spermatozoa after 1 hour and 3 hours of treatment. The effects of human FF on beat cross-frequency and hyperactivation were dose-dependent. Conclusion(s): Human FF promotes capacitation and the acrosome reaction within a short period. It also stimulates or maintains various sperm motility parameters. Copyright (C) 2000 American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectAcrosome reactionen_HK
dc.subjectCapacitationen_HK
dc.subjectHuman follicular fluiden_HK
dc.subjectMotilityen_HK
dc.subjectSpermatozoaen_HK
dc.titleEffects of human follicular fluid on the capacitation and motility of human spermatozoaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=73&issue=4&spage=680&epage=686&date=2000&atitle=Effects+of+human+follicular+fluid+on+the+capacitation+and+motility+of+human+spermatozoaen_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(99)00637-8en_HK
dc.identifier.pmid10731525en_HK
dc.identifier.scopuseid_2-s2.0-0034069417en_HK
dc.identifier.hkuros49685en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034069417&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume73en_HK
dc.identifier.issue4en_HK
dc.identifier.spage680en_HK
dc.identifier.epage686en_HK
dc.identifier.isiWOS:000086043700003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats