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Article: Effect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryos

TitleEffect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryos
Authors
Issue Date2007
Citation
Zhonghua Fu Chan Ke Za Zhi, 2007, v. 42 n. 9, p. 605-607 How to Cite?
AbstractOBJECTIVE: To investigate the effect of embryotrophic factors (ETF) from human oviductal cells on gene expression of mouse early developmental embryos and discuss the role of fallopian tube in early development of embryos. METHODS: ETF was isolated from conditioned medium of human oviductal cell line by sequential liquid chromatographic systems. Mouse embryos were treated by ETF in vitro. Using differential display RT-PCR, the gene expression of embryos treated by ETF was compared with embryos without ETF treatment. The differentially expressed genes were separated, re-amplified, cloned and sequenced. RESULTS: Gene expression profiles of embryos with ETF treatment was different from embryos without this treatment. Eight differentially expressed genes were cloned and sequenced. These genes functioned in RNA degradation, synthesis, splicing, protein trafficking, cellular differentiation and embryo development. CONCLUSION: Embryotrophic factors from human oviductal cells affect gene expression of early developmental embryos. The human oviductal cells play wide roles in early developmental stages of embryos.
Persistent Identifierhttp://hdl.handle.net/10722/87024
ISSN
2015 SCImago Journal Rankings: 0.168

 

DC FieldValueLanguage
dc.contributor.authorYao, YQen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorXu, JSen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, SBen_HK
dc.date.accessioned2010-09-06T09:24:19Z-
dc.date.available2010-09-06T09:24:19Z-
dc.date.issued2007en_HK
dc.identifier.citationZhonghua Fu Chan Ke Za Zhi, 2007, v. 42 n. 9, p. 605-607en_HK
dc.identifier.issn0529-567Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87024-
dc.description.abstractOBJECTIVE: To investigate the effect of embryotrophic factors (ETF) from human oviductal cells on gene expression of mouse early developmental embryos and discuss the role of fallopian tube in early development of embryos. METHODS: ETF was isolated from conditioned medium of human oviductal cell line by sequential liquid chromatographic systems. Mouse embryos were treated by ETF in vitro. Using differential display RT-PCR, the gene expression of embryos treated by ETF was compared with embryos without ETF treatment. The differentially expressed genes were separated, re-amplified, cloned and sequenced. RESULTS: Gene expression profiles of embryos with ETF treatment was different from embryos without this treatment. Eight differentially expressed genes were cloned and sequenced. These genes functioned in RNA degradation, synthesis, splicing, protein trafficking, cellular differentiation and embryo development. CONCLUSION: Embryotrophic factors from human oviductal cells affect gene expression of early developmental embryos. The human oviductal cells play wide roles in early developmental stages of embryos.en_HK
dc.languageengen_HK
dc.relation.ispartofZhonghua fu chan ke za zhien_HK
dc.titleEffect of human oviductal embryotrophic factors on gene expression of mouse preimplantation embryosen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, SB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, SB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17983515en_HK
dc.identifier.scopuseid_2-s2.0-58849152858en_HK
dc.identifier.hkuros148775en_HK
dc.identifier.volume42en_HK
dc.identifier.issue9en_HK
dc.identifier.spage605en_HK
dc.identifier.epage607en_HK
dc.identifier.scopusauthoridYao, YQ=7403567431en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridXu, JS=35083004300en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, SB=7102370745en_HK

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