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Article: Cumulus cells reduce the spermatozoa-zona binding inhibitory activity of human follicular fluid

TitleCumulus cells reduce the spermatozoa-zona binding inhibitory activity of human follicular fluid
Authors
KeywordsCumulus cells
Follicular fluid
Motility
Spermatozoa
Zona pellucida binding
Issue Date2003
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2003, v. 79 n. 3 SUPPL. 1, p. 802-807 How to Cite?
AbstractObjective: To investigate the effects of human follicular fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction program and men visiting the subfertility clinics. Intervention(s): Culture medium and human follicular fluid were used to culture cumulus cells in vitro for specified time periods. Main outcome measure(s): Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human follicular fluid. Result(s): Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human follicular fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human follicular fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human follicular fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Conclusion(s): Cumulus cells reduced the inhibitory effect of human follicular fluid on spermatozoa-zona binding in vitro in a time-dependent manner. © 2003 by American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/87019
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHong, SJen_HK
dc.contributor.authorTse, JYMen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:24:15Z-
dc.date.available2010-09-06T09:24:15Z-
dc.date.issued2003en_HK
dc.identifier.citationFertility And Sterility, 2003, v. 79 n. 3 SUPPL. 1, p. 802-807en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87019-
dc.description.abstractObjective: To investigate the effects of human follicular fluid cultured with cumulus cells to inhibit the binding of spermatozoa to the zona pellucida of oocytes. Design: Controlled experimental laboratory study. Setting: University gynecology unit. Patient(s): Women undergoing assisted reproduction program and men visiting the subfertility clinics. Intervention(s): Culture medium and human follicular fluid were used to culture cumulus cells in vitro for specified time periods. Main outcome measure(s): Zona binding capacity and motility of spermatozoa after incubation with cumulus cells treated culture medium or human follicular fluid. Result(s): Compared with the control medium, spent culture media after culturing cumulus cells for 3, 5, and 7 hours did not affect the motility and zona binding capacity of the treated spermatozoa. Significantly more spermatozoa treated with human follicular fluid that had been preincubated with cumulus cells for 5 and 7 hours bound onto hemizona in hemizona binding assay when compared with those preincubated in human follicular fluid without cumulus treatment. The hemizona index increased with the increase in the duration of cumulus cell treatment. Human follicular fluid with or without cumulus cells maintained sperm motility to similar extent for 3 hours. Conclusion(s): Cumulus cells reduced the inhibitory effect of human follicular fluid on spermatozoa-zona binding in vitro in a time-dependent manner. © 2003 by American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectCumulus cellsen_HK
dc.subjectFollicular fluiden_HK
dc.subjectMotilityen_HK
dc.subjectSpermatozoaen_HK
dc.subjectZona pellucida bindingen_HK
dc.titleCumulus cells reduce the spermatozoa-zona binding inhibitory activity of human follicular fluiden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=79&issue=suppl 1&spage=802&epage=807&date=2003&atitle=Cumulus+cells+reduce+the+spermatozoa-zona+binding+inhibitory+activity+of+human+follicular+fluiden_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0015-0282(02)04832-Xen_HK
dc.identifier.pmid12620494-
dc.identifier.scopuseid_2-s2.0-0037374245en_HK
dc.identifier.hkuros92144en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037374245&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue3 SUPPL. 1en_HK
dc.identifier.spage802en_HK
dc.identifier.epage807en_HK
dc.identifier.isiWOS:000181670300021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHong, SJ=7405767201en_HK
dc.identifier.scopusauthoridTse, JYM=7102607314en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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