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Article: Complement 3 deficiency impairs early pregnancy in mice

TitleComplement 3 deficiency impairs early pregnancy in mice
Authors
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2009, v. 76 n. 7, p. 647-655 How to Cite?
AbstractHuman oviductal cells produce complement-3 (C3) and its derivative, iC3b. These molecules are important in immune responses. Our recent study suggested that iC3b also possessed embryotrophic activity and it stimulates the blastulation and hatching rates of in vitro cultured mouse embryos. The objective is to study the impact of C3 deficiency on early pregnancy in vivo using homozygous C3-deficient (C3KO) and wild-type (C3WT) mice. C3 protein was undetectable in the reproductive tissues of C3KO mice. Deficiency in C3 is associated with significantly longer estrous cycle (P = 0.037). No significant difference was found in the ovulation rate, total cell count in blastocysts and implantation rate between the wild-type and the C3KO mice, though C3KO mice tended to have lower values in the latter two parameters. On day 15 of pregnancy, C3KO mice had fewer conceptus (P < 0.001) and higher resorption rate (P < 0.001) than that of C3WT mice. The fetal and placental weights (P < 0.001) were lower in the C3KO mice. The placenta of C3KO mice had smaller spongiotrophoblast (P = 0.001) and labyrinth (P = 0.037). Deficiency in C3 is associated with mild impairment in early pregnancy including longer estrous cycle and higher resorption rates after implantation. The impairment may be related to compromised placental development leading to under-developed fetuses. © 2009 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/87010
ISSN
2014 Impact Factor: 2.527
2014 SCImago Journal Rankings: 0.975
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council, Hong KongHKU7319/01M
HKU7411/04M
Funding Information:

This work was supported in part by grants (HKU7319/01M to W.S.B.Y. and HKU7411/04M to K.F.L.) from the Research Grant Council, Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChow, WNen_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorWong, PCen_HK
dc.contributor.authorChung, MKen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-09-06T09:24:08Z-
dc.date.available2010-09-06T09:24:08Z-
dc.date.issued2009en_HK
dc.identifier.citationMolecular Reproduction And Development, 2009, v. 76 n. 7, p. 647-655en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/87010-
dc.description.abstractHuman oviductal cells produce complement-3 (C3) and its derivative, iC3b. These molecules are important in immune responses. Our recent study suggested that iC3b also possessed embryotrophic activity and it stimulates the blastulation and hatching rates of in vitro cultured mouse embryos. The objective is to study the impact of C3 deficiency on early pregnancy in vivo using homozygous C3-deficient (C3KO) and wild-type (C3WT) mice. C3 protein was undetectable in the reproductive tissues of C3KO mice. Deficiency in C3 is associated with significantly longer estrous cycle (P = 0.037). No significant difference was found in the ovulation rate, total cell count in blastocysts and implantation rate between the wild-type and the C3KO mice, though C3KO mice tended to have lower values in the latter two parameters. On day 15 of pregnancy, C3KO mice had fewer conceptus (P < 0.001) and higher resorption rate (P < 0.001) than that of C3WT mice. The fetal and placental weights (P < 0.001) were lower in the C3KO mice. The placenta of C3KO mice had smaller spongiotrophoblast (P = 0.001) and labyrinth (P = 0.037). Deficiency in C3 is associated with mild impairment in early pregnancy including longer estrous cycle and higher resorption rates after implantation. The impairment may be related to compromised placental development leading to under-developed fetuses. © 2009 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.rightsMolecular Reproduction and Development. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleComplement 3 deficiency impairs early pregnancy in miceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1040-452X&volume=76&spage=647&epage=55&date=2009&atitle=Complement+3+deficiency+impairs+early+pregnancy+in+mice.en_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrd.21013en_HK
dc.identifier.pmid19205046en_HK
dc.identifier.scopuseid_2-s2.0-67449106491en_HK
dc.identifier.hkuros158112en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67449106491&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume76en_HK
dc.identifier.issue7en_HK
dc.identifier.spage647en_HK
dc.identifier.epage655en_HK
dc.identifier.isiWOS:000266517200007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, WN=36852688700en_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridWong, PC=35094888100en_HK
dc.identifier.scopusauthoridChung, MK=8964203600en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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