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Article: Increased lymphocyte DNA strand breaks in rubber workers

TitleIncreased lymphocyte DNA strand breaks in rubber workers
Authors
Issue Date2000
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gentox
Citation
Mutation Research - Genetic Toxicology And Environmental Mutagenesis, 2000, v. 470 n. 2, p. 201-209 How to Cite?
AbstractObjective: To study the effect of occupational exposure to rubber processing, smoking, and alcohol drinking on lymphocyte DNA damage. Subjects and Methods: Of 371 employees (197 men and 174 women) from a rubber factory in Guangzhou, 281 were rubber processing workers from five production sections and 90 were managerial workers. Information on occupational exposure, smoking, and drinking was collected by interviews. Blood samples were taken in the morning by venipuncture. DNA damages were measured by the Comet assay. Possible DNA-protein crosslinks were broken down by proteinase K. Tail moment, measured by Komet 4.0 image analysis software, was the measure of DNA damage. Results: The rubber processing workers had larger tail moment than the managerial workers (Geometric mean, 95%CI) [1.77 μm (1.64- 1.90) versus 1.52 μm (1.36-1.71), P = 0.04]. Both smoking [1.93 μm (1.74- 2.13) versus 1.59 μm (1.47-1.71), P = 0.003] and alcohol drinking [2.21 μm (1.87-2.62) versus 1.63 μm (1.53-1.74), P < 0.001] increased tail moment. Tail moment differed significantly among job categories (F = 3.21, P = 0.008), the largest was observed in mixers. In the non-smoking and non- drinking workers, rubber processing workers had larger tail moment than managerial workers after adjusting for age (P = 0.033). General linear model analysis showed that after adjusting for each other, occupational exposure (P = 0.027), smoking (P = 0.012), and alcohol drinking (P = 0.013) was associated with larger tail moment, whereas age and gender had no effect. Conclusions: Occupational exposure to rubber processing, smoking, and alcohol drinking can cause DNA damage. (C) 2000 Elsevier Science B.V.
Persistent Identifierhttp://hdl.handle.net/10722/86702
ISSN
2015 Impact Factor: 2.254
2015 SCImago Journal Rankings: 1.052
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, CQen_HK
dc.contributor.authorLam, THen_HK
dc.contributor.authorJiang, CQen_HK
dc.contributor.authorWei, BXen_HK
dc.contributor.authorXu, QRen_HK
dc.contributor.authorChen, YHen_HK
dc.date.accessioned2010-09-06T09:20:17Z-
dc.date.available2010-09-06T09:20:17Z-
dc.date.issued2000en_HK
dc.identifier.citationMutation Research - Genetic Toxicology And Environmental Mutagenesis, 2000, v. 470 n. 2, p. 201-209en_HK
dc.identifier.issn1383-5718en_HK
dc.identifier.urihttp://hdl.handle.net/10722/86702-
dc.description.abstractObjective: To study the effect of occupational exposure to rubber processing, smoking, and alcohol drinking on lymphocyte DNA damage. Subjects and Methods: Of 371 employees (197 men and 174 women) from a rubber factory in Guangzhou, 281 were rubber processing workers from five production sections and 90 were managerial workers. Information on occupational exposure, smoking, and drinking was collected by interviews. Blood samples were taken in the morning by venipuncture. DNA damages were measured by the Comet assay. Possible DNA-protein crosslinks were broken down by proteinase K. Tail moment, measured by Komet 4.0 image analysis software, was the measure of DNA damage. Results: The rubber processing workers had larger tail moment than the managerial workers (Geometric mean, 95%CI) [1.77 μm (1.64- 1.90) versus 1.52 μm (1.36-1.71), P = 0.04]. Both smoking [1.93 μm (1.74- 2.13) versus 1.59 μm (1.47-1.71), P = 0.003] and alcohol drinking [2.21 μm (1.87-2.62) versus 1.63 μm (1.53-1.74), P < 0.001] increased tail moment. Tail moment differed significantly among job categories (F = 3.21, P = 0.008), the largest was observed in mixers. In the non-smoking and non- drinking workers, rubber processing workers had larger tail moment than managerial workers after adjusting for age (P = 0.033). General linear model analysis showed that after adjusting for each other, occupational exposure (P = 0.027), smoking (P = 0.012), and alcohol drinking (P = 0.013) was associated with larger tail moment, whereas age and gender had no effect. Conclusions: Occupational exposure to rubber processing, smoking, and alcohol drinking can cause DNA damage. (C) 2000 Elsevier Science B.V.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gentoxen_HK
dc.relation.ispartofMutation Research - Genetic Toxicology and Environmental Mutagenesisen_HK
dc.rightsMutation Research. Copyright © Elsevier BV.en_HK
dc.subject.meshComet Assayen_HK
dc.subject.meshDNA Damageen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLymphocytes - ultrastructureen_HK
dc.subject.meshMaleen_HK
dc.subject.meshOccupational Exposureen_HK
dc.subject.meshRubberen_HK
dc.titleIncreased lymphocyte DNA strand breaks in rubber workersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0027-5107&volume=470&spage=201&epage=9&date=2000&atitle=Increased+lymphocyte+DNA+strand+breaks+in+rubber+workersen_HK
dc.identifier.emailLam, TH:hrmrlth@hkucc.hku.hken_HK
dc.identifier.authorityLam, TH=rp00326en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1383-5718(00)00106-6en_HK
dc.identifier.pmid11027975-
dc.identifier.scopuseid_2-s2.0-0342794229en_HK
dc.identifier.hkuros55785en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0342794229&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume470en_HK
dc.identifier.issue2en_HK
dc.identifier.spage201en_HK
dc.identifier.epage209en_HK
dc.identifier.isiWOS:000090151200014-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhu, CQ=8537385600en_HK
dc.identifier.scopusauthoridLam, TH=7202522876en_HK
dc.identifier.scopusauthoridJiang, CQ=10639500500en_HK
dc.identifier.scopusauthoridWei, BX=8138124100en_HK
dc.identifier.scopusauthoridXu, QR=7403743673en_HK
dc.identifier.scopusauthoridChen, YH=8138124300en_HK

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