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Article: Characterization of ionic currents in human mesenchymal stem cells from bone marrow

TitleCharacterization of ionic currents in human mesenchymal stem cells from bone marrow
Authors
Issue Date2005
PublisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.com
Citation
Stem Cells, 2005, v. 23 n. 3, p. 371-382 How to Cite?
AbstractThis study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca2+-activated K+ channel (IKCa) blocker iberiotoxin, a transient outward K+ current (Ito) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K+ current (IKDR)-like ether-à-go-go (eag) K+ channel. In addition, tetrodotoxin-sensitive sodium current (INa.TTX) and nifedipine-sensitive L-type Ca2+ current (ICa.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for IKCa, Kv4.2 and Kv1.4 for Ito, heag1 for IKDR, hNE-Na for INa.TTX, and CACNAIC for I Ca.L. These results demonstrate that multiple functional ion channel currents-that is, IKca, Ito heag1, INa.TTX, and ICa.L-are expressed in hMSCs from bone marrow.
Persistent Identifierhttp://hdl.handle.net/10722/85513
ISSN
2015 Impact Factor: 5.902
2015 SCImago Journal Rankings: 3.438
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_HK
dc.contributor.authorSun, Hen_HK
dc.contributor.authorDeng, Xen_HK
dc.contributor.authorLau, CPen_HK
dc.date.accessioned2010-09-06T09:05:57Z-
dc.date.available2010-09-06T09:05:57Z-
dc.date.issued2005en_HK
dc.identifier.citationStem Cells, 2005, v. 23 n. 3, p. 371-382en_HK
dc.identifier.issn1066-5099en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85513-
dc.description.abstractThis study characterized functional ion channels in cultured undifferentiated human mesenchymal stem cells (hMSCs) from bone marrow with whole-cell patch clamp and reverse transcription polymerase chain reaction (RT-PCR) techniques. Three types of outward currents were found in hMSCs, including a noise-like rapidly activating outward current inhibited by the large conductance Ca2+-activated K+ channel (IKCa) blocker iberiotoxin, a transient outward K+ current (Ito) suppressed by 4-aminopyridine (4-AP), and a delayed rectifier K+ current (IKDR)-like ether-à-go-go (eag) K+ channel. In addition, tetrodotoxin-sensitive sodium current (INa.TTX) and nifedipine-sensitive L-type Ca2+ current (ICa.L) were also detected in 29% and 15% hMSCs, respectively. Moreover, RT-PCR revealed the molecular evidence of high levels of mRNA for the functional ionic currents, including human MaxiK for IKCa, Kv4.2 and Kv1.4 for Ito, heag1 for IKDR, hNE-Na for INa.TTX, and CACNAIC for I Ca.L. These results demonstrate that multiple functional ion channel currents-that is, IKca, Ito heag1, INa.TTX, and ICa.L-are expressed in hMSCs from bone marrow.en_HK
dc.languageengen_HK
dc.publisherAlphaMed Press, Inc. The Journal's web site is located at http://www.stemcells.comen_HK
dc.relation.ispartofStem Cellsen_HK
dc.subject.mesh4-Aminopyridine - pharmacologyen_HK
dc.subject.meshBone Marrow Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshCalcium Channels, L-Type - drug effects - genetics - physiologyen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshEther-A-Go-Go Potassium Channelsen_HK
dc.subject.meshGene Expression - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIon Channels - genetics - physiologyen_HK
dc.subject.meshKv1.4 Potassium Channelen_HK
dc.subject.meshLarge-Conductance Calcium-Activated Potassium Channel alpha Subunitsen_HK
dc.subject.meshLarge-Conductance Calcium-Activated Potassium Channelsen_HK
dc.subject.meshMembrane Potentials - drug effectsen_HK
dc.subject.meshMesenchymal Stem Cells - cytology - drug effects - physiologyen_HK
dc.subject.meshNerve Tissue Proteins - geneticsen_HK
dc.subject.meshNifedipine - pharmacologyen_HK
dc.subject.meshPatch-Clamp Techniquesen_HK
dc.subject.meshPeptides - pharmacologyen_HK
dc.subject.meshPotassium Channels - geneticsen_HK
dc.subject.meshPotassium Channels, Calcium-Activated - drug effects - genetics - physiologyen_HK
dc.subject.meshPotassium Channels, Voltage-Gated - drug effects - genetics - physiologyen_HK
dc.subject.meshRNA, Messenger - genetics - metabolismen_HK
dc.subject.meshShal Potassium Channelsen_HK
dc.subject.meshSodium Channels - drug effects - genetics - physiologyen_HK
dc.subject.meshTetrodotoxin - pharmacologyen_HK
dc.titleCharacterization of ionic currents in human mesenchymal stem cells from bone marrowen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1066-5099&volume=23&issue=3&spage=371&epage=382&date=2005&atitle=Characterization+of+ionic+currents+in+human+mesenchymal+stem+cells+from+bone+marrowen_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1634/stemcells.2004-0213en_HK
dc.identifier.pmid15749932-
dc.identifier.scopuseid_2-s2.0-15544379480en_HK
dc.identifier.hkuros101185en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15544379480&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue3en_HK
dc.identifier.spage371en_HK
dc.identifier.epage382en_HK
dc.identifier.isiWOS:000227786900010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.scopusauthoridSun, H=35723049200en_HK
dc.identifier.scopusauthoridDeng, X=14057894600en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK

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