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Article: Direct detection of cytolytic T lymphocyte-mediated cytotoxicity on antigen-transfected cell microarray

TitleDirect detection of cytolytic T lymphocyte-mediated cytotoxicity on antigen-transfected cell microarray
Authors
KeywordsCaspases
CD8+ T lymphocytes
Cell-mediated cytotoxicity
Imaging
Microarray
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jim
Citation
Journal Of Immunological Methods, 2007, v. 326 n. 1-2, p. 1-9 How to Cite?
AbstractCD8+ T lymphocytes are capable of recognizing and destroying cancer cells or virally infected cells and can thus offer protection from cellular malignant transformation and pathogenic challenges. With large numbers of genes discovered in genome analyses, rapid identification of cancer or viral antigens would facilitate better exploitation of CD8+ T lymphocyte-mediated immune protection. Reverse transfection microarray technology allows expression of individual cDNAs at defined positions in a cell monolayer and direct detection of corresponding phenotypic changes of transfected cells at specific locations. In this study, we have integrated reverse transfection with image-based fluorometric detection of antigen-specific CTL-mediated cytotoxicity on transfected cell microarrays. As a result, the antigen recognition of cloned CTL cells has been successfully detected on the cell microarray, in which the specific or non-specific mini-gene DNA in the expression vector or vector alone was spotted. Moreover, the cellular capability of antigen processing and presentation on microarray has also been evaluated by using chimeric DNA constructs containing the antigen-encoding mini-gene sequence. This novel approach may facilitate high throughput screens of cancer cell or virus cDNA libraries to identify individual cDNAs that encode targets for immune intervention. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/85488
ISSN
2015 Impact Factor: 1.858
2015 SCImago Journal Rankings: 1.053
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRong, Jen_HK
dc.contributor.authorChris Bleackley, Ren_HK
dc.contributor.authorKane, KPen_HK
dc.date.accessioned2010-09-06T09:05:40Z-
dc.date.available2010-09-06T09:05:40Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Immunological Methods, 2007, v. 326 n. 1-2, p. 1-9en_HK
dc.identifier.issn0022-1759en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85488-
dc.description.abstractCD8+ T lymphocytes are capable of recognizing and destroying cancer cells or virally infected cells and can thus offer protection from cellular malignant transformation and pathogenic challenges. With large numbers of genes discovered in genome analyses, rapid identification of cancer or viral antigens would facilitate better exploitation of CD8+ T lymphocyte-mediated immune protection. Reverse transfection microarray technology allows expression of individual cDNAs at defined positions in a cell monolayer and direct detection of corresponding phenotypic changes of transfected cells at specific locations. In this study, we have integrated reverse transfection with image-based fluorometric detection of antigen-specific CTL-mediated cytotoxicity on transfected cell microarrays. As a result, the antigen recognition of cloned CTL cells has been successfully detected on the cell microarray, in which the specific or non-specific mini-gene DNA in the expression vector or vector alone was spotted. Moreover, the cellular capability of antigen processing and presentation on microarray has also been evaluated by using chimeric DNA constructs containing the antigen-encoding mini-gene sequence. This novel approach may facilitate high throughput screens of cancer cell or virus cDNA libraries to identify individual cDNAs that encode targets for immune intervention. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jimen_HK
dc.relation.ispartofJournal of Immunological Methodsen_HK
dc.rightsJournal of Immunological Methods. Copyright © Elsevier BV.en_HK
dc.subjectCaspasesen_HK
dc.subjectCD8+ T lymphocytesen_HK
dc.subjectCell-mediated cytotoxicityen_HK
dc.subjectImagingen_HK
dc.subjectMicroarrayen_HK
dc.titleDirect detection of cytolytic T lymphocyte-mediated cytotoxicity on antigen-transfected cell microarrayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-1759&volume=326&spage=1&epage=6&date=2007&atitle=Direct+Detection+of+Cytolytic+T+Lymphocyte-Mediated+Cytotoxicity+on+Antigen-Transfected+Cell+Microarrayen_HK
dc.identifier.emailRong, J: jrong@hku.hken_HK
dc.identifier.authorityRong, J=rp00515en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jim.2007.06.008en_HK
dc.identifier.pmid17673228-
dc.identifier.scopuseid_2-s2.0-34548516138en_HK
dc.identifier.hkuros130744en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548516138&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume326en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage1en_HK
dc.identifier.epage9en_HK
dc.identifier.isiWOS:000250026500001-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridRong, J=7005980047en_HK
dc.identifier.scopusauthoridChris Bleackley, R=6508134465en_HK
dc.identifier.scopusauthoridKane, KP=24534271300en_HK

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