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Article: Influence of interleukin-2 on Ca2+ handling in rat ventricular myocytes

TitleInfluence of interleukin-2 on Ca2+ handling in rat ventricular myocytes
Authors
KeywordsCardiomyocytes
Frequency relationship
Interleukin-2
Intracellular Ca2+
Issue Date2003
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmcc
Citation
Journal Of Molecular And Cellular Cardiology, 2003, v. 35 n. 12, p. 1491-1503 How to Cite?
AbstractIn the present study, we examined the effect of interleukin-2 (IL-2) on cardiomyocyte Ca2+ handling. The effects of steady-state and transient changes in stimulation frequency on the intracellular Ca2+ transient were investigated in isolated ventricular myocytes by spectrofluorometry. In the steady state (0.2 Hz) IL-2 (200 U/ml) decreased the amplitude of Ca2+ transients induced by electrical stimulation and caffeine. At 1.25 mM extracellular Ca2+ concentration ([Ca 2+]o), when the stimulation frequency increased from 0.2 to 1.0 Hz, diastolic Ca2+ level and peak intracellular Ca 2+ concentration ([Ca2+]i), as well as the amplitude of the transient, increased. The positive frequency relationships of the peak and amplitude of [Ca2+]i transients were blunted in the IL-2-treated myocytes. The effect of IL-2 on the electrically induced [Ca2+]i transient was not normalized by increasing [Ca2+]o to 2.5 mM. IL-2 inhibited the frequency relationship of caffeine-induced Ca2+ release. Blockade of sarcoplasmic reticulum (SR) Ca2+-ATPase with thapsigargin resulted in a significant reduction of the amplitude-frequency relationship of the transient similar to that induced by IL-2. The restitutions were not different between control and IL-2 groups at 1.25 mM [Ca2+]o, which was slowed in IL-2-treated myocytes when [Ca2+]o was increased to 2.5 mM. There was no difference in the recirculation fraction (RF) between control and IL-2-treated myocytes at both 1.25 and 2.5 mM [Ca 2+]o. The effects of IL-2 on frequency relationship, restitution, and RF may be due to depressed SR functions and an increased Na+-Ca2+ exchange activity, but not to any change in L-type Ca2+ channels. © 2003 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/85484
ISSN
2015 Impact Factor: 4.874
2015 SCImago Journal Rankings: 2.522
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCao, CMen_HK
dc.contributor.authorXia, Qen_HK
dc.contributor.authorBruce, ICen_HK
dc.contributor.authorShen, YLen_HK
dc.contributor.authorYe, ZGen_HK
dc.contributor.authorLin, GHen_HK
dc.contributor.authorChen, JZen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-09-06T09:05:37Z-
dc.date.available2010-09-06T09:05:37Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Molecular And Cellular Cardiology, 2003, v. 35 n. 12, p. 1491-1503en_HK
dc.identifier.issn0022-2828en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85484-
dc.description.abstractIn the present study, we examined the effect of interleukin-2 (IL-2) on cardiomyocyte Ca2+ handling. The effects of steady-state and transient changes in stimulation frequency on the intracellular Ca2+ transient were investigated in isolated ventricular myocytes by spectrofluorometry. In the steady state (0.2 Hz) IL-2 (200 U/ml) decreased the amplitude of Ca2+ transients induced by electrical stimulation and caffeine. At 1.25 mM extracellular Ca2+ concentration ([Ca 2+]o), when the stimulation frequency increased from 0.2 to 1.0 Hz, diastolic Ca2+ level and peak intracellular Ca 2+ concentration ([Ca2+]i), as well as the amplitude of the transient, increased. The positive frequency relationships of the peak and amplitude of [Ca2+]i transients were blunted in the IL-2-treated myocytes. The effect of IL-2 on the electrically induced [Ca2+]i transient was not normalized by increasing [Ca2+]o to 2.5 mM. IL-2 inhibited the frequency relationship of caffeine-induced Ca2+ release. Blockade of sarcoplasmic reticulum (SR) Ca2+-ATPase with thapsigargin resulted in a significant reduction of the amplitude-frequency relationship of the transient similar to that induced by IL-2. The restitutions were not different between control and IL-2 groups at 1.25 mM [Ca2+]o, which was slowed in IL-2-treated myocytes when [Ca2+]o was increased to 2.5 mM. There was no difference in the recirculation fraction (RF) between control and IL-2-treated myocytes at both 1.25 and 2.5 mM [Ca 2+]o. The effects of IL-2 on frequency relationship, restitution, and RF may be due to depressed SR functions and an increased Na+-Ca2+ exchange activity, but not to any change in L-type Ca2+ channels. © 2003 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjmccen_HK
dc.relation.ispartofJournal of Molecular and Cellular Cardiologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCardiomyocytesen_HK
dc.subjectFrequency relationshipen_HK
dc.subjectInterleukin-2en_HK
dc.subjectIntracellular Ca2+en_HK
dc.subject.meshAdenosine Triphosphatases - drug effects - metabolism-
dc.subject.meshCaffeine/pharmacology-
dc.subject.meshCalcium - metabolism-
dc.subject.meshInterleukin-2 - pharmacology-
dc.subject.meshMyocytes, Cardiac - cytology - drug effects - metabolism-
dc.titleInfluence of interleukin-2 on Ca2+ handling in rat ventricular myocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2828&volume=35&issue=12&spage=1491&epage=1503&date=2003&atitle=Influence+of+interleukin-2+on+Ca2++handling+in+rat+ventricular+myocytes.en_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.yjmcc.2003.09.017en_HK
dc.identifier.pmid14654375-
dc.identifier.scopuseid_2-s2.0-0344062668en_HK
dc.identifier.hkuros93503en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0344062668&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1491en_HK
dc.identifier.epage1503en_HK
dc.identifier.isiWOS:000187625100012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCao, CM=7401501737en_HK
dc.identifier.scopusauthoridXia, Q=7202871577en_HK
dc.identifier.scopusauthoridBruce, IC=35612490700en_HK
dc.identifier.scopusauthoridShen, YL=35082647400en_HK
dc.identifier.scopusauthoridYe, ZG=7401956920en_HK
dc.identifier.scopusauthoridLin, GH=7401699746en_HK
dc.identifier.scopusauthoridChen, JZ=36107692700en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK

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