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Article: Heterochromatin effects on the frequency and duration of LCR-mediated gene transcription

TitleHeterochromatin effects on the frequency and duration of LCR-mediated gene transcription
Authors
Issue Date1996
PublisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cell
Citation
Cell, 1996, v. 87 n. 1, p. 105-114 How to Cite?
AbstractLocus control regions (LCRs) are responsible for initiating and maintaining a stable tissue-specific open chromatin structure of a locus. In transgenic mice, LCRs confer high level expression on linked genes independent of position in the mouse genome. Here we show that an incomplete LCR loses this property when integrated into heterochromatic regions. Two disruption mechanisms were observed. One is classical position-effect variegation, resulting in continuous transcription in a clonal subpopulation of cells. The other is a novel mechanism resulting in intermittent gene transcription in all cells. We conclude that only a complete LCR fully overcomes heterochromatin silencing and that it controls the level of transcription by ensuring activity in all cells at all times rather than directly controlling the rate of transcription.
Persistent Identifierhttp://hdl.handle.net/10722/85473
ISSN
2015 Impact Factor: 28.71
2015 SCImago Journal Rankings: 28.188
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMilot, Een_HK
dc.contributor.authorStrouboulis, Jen_HK
dc.contributor.authorTrimborn, Ten_HK
dc.contributor.authorWijgerde, Men_HK
dc.contributor.authorDe Boer, Een_HK
dc.contributor.authorLangeveld, Aen_HK
dc.contributor.authorTanUn, Ken_HK
dc.contributor.authorVergeer, Wen_HK
dc.contributor.authorYannoutsos, Nen_HK
dc.contributor.authorGrosveld, Fen_HK
dc.contributor.authorFraser, Pen_HK
dc.date.accessioned2010-09-06T09:05:25Z-
dc.date.available2010-09-06T09:05:25Z-
dc.date.issued1996en_HK
dc.identifier.citationCell, 1996, v. 87 n. 1, p. 105-114en_HK
dc.identifier.issn0092-8674en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85473-
dc.description.abstractLocus control regions (LCRs) are responsible for initiating and maintaining a stable tissue-specific open chromatin structure of a locus. In transgenic mice, LCRs confer high level expression on linked genes independent of position in the mouse genome. Here we show that an incomplete LCR loses this property when integrated into heterochromatic regions. Two disruption mechanisms were observed. One is classical position-effect variegation, resulting in continuous transcription in a clonal subpopulation of cells. The other is a novel mechanism resulting in intermittent gene transcription in all cells. We conclude that only a complete LCR fully overcomes heterochromatin silencing and that it controls the level of transcription by ensuring activity in all cells at all times rather than directly controlling the rate of transcription.en_HK
dc.languageengen_HK
dc.publisherCell Press. The Journal's web site is located at http://www.elsevier.com/locate/cellen_HK
dc.relation.ispartofCellen_HK
dc.titleHeterochromatin effects on the frequency and duration of LCR-mediated gene transcriptionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0092-8674&volume=87&spage=105&epage=114&date=1996&atitle=Heterochromatin+Effects+on+the+Frequency+and+Duration+of+LCR-Mediated+Gene+Transcriptionen_HK
dc.identifier.emailTan-Un, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityTan-Un, KC=rp00787en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0092-8674(00)81327-6en_HK
dc.identifier.pmid8858153-
dc.identifier.scopuseid_2-s2.0-16044372786en_HK
dc.identifier.hkuros27452en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-16044372786&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue1en_HK
dc.identifier.spage105en_HK
dc.identifier.epage114en_HK
dc.identifier.isiWOS:A1996VL69500013-
dc.publisher.placeUnited Statesen_HK

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