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Article: High-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
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TitleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
 
AuthorsTai, ALS
Mak, W1
Ng, PKM1
Chua, DTT
Ng, MYM1
Fu, L
Chu, KKW
Fang, Y2
You, QS1
Chen, M
Zhang, M
Sham, PC1
Guan, XY2 1
 
Issue Date2006
 
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
 
CitationCancer Research, 2006, v. 66 n. 8, p. 4133-4138 [How to Cite?]
DOI: http://dx.doi.org/10.1158/0008-5472.CAN-05-2775
 
AbstractLoss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.
 
ISSN0008-5472
2012 Impact Factor: 8.65
2012 SCImago Journal Rankings: 4.152
 
DOIhttp://dx.doi.org/10.1158/0008-5472.CAN-05-2775
 
ISI Accession Number IDWOS:000236843200025
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTai, ALS
 
dc.contributor.authorMak, W
 
dc.contributor.authorNg, PKM
 
dc.contributor.authorChua, DTT
 
dc.contributor.authorNg, MYM
 
dc.contributor.authorFu, L
 
dc.contributor.authorChu, KKW
 
dc.contributor.authorFang, Y
 
dc.contributor.authorYou, QS
 
dc.contributor.authorChen, M
 
dc.contributor.authorZhang, M
 
dc.contributor.authorSham, PC
 
dc.contributor.authorGuan, XY
 
dc.date.accessioned2010-09-06T09:05:15Z
 
dc.date.available2010-09-06T09:05:15Z
 
dc.date.issued2006
 
dc.description.abstractLoss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCancer Research, 2006, v. 66 n. 8, p. 4133-4138 [How to Cite?]
DOI: http://dx.doi.org/10.1158/0008-5472.CAN-05-2775
 
dc.identifier.doihttp://dx.doi.org/10.1158/0008-5472.CAN-05-2775
 
dc.identifier.epage4138
 
dc.identifier.hkuros115336
 
dc.identifier.hkuros128682
 
dc.identifier.isiWOS:000236843200025
 
dc.identifier.issn0008-5472
2012 Impact Factor: 8.65
2012 SCImago Journal Rankings: 4.152
 
dc.identifier.issue8
 
dc.identifier.openurl
 
dc.identifier.pmid16618734
 
dc.identifier.scopuseid_2-s2.0-33646241534
 
dc.identifier.spage4133
 
dc.identifier.urihttp://hdl.handle.net/10722/85466
 
dc.identifier.volume66
 
dc.languageeng
 
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofCancer Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics
 
dc.subject.meshChromosomes, Human, Pair 3 - genetics
 
dc.subject.meshGenes, Tumor Suppressor
 
dc.subject.meshGenotype
 
dc.subject.meshHumans
 
dc.subject.meshLoss of Heterozygosity
 
dc.subject.meshLung Neoplasms - genetics
 
dc.subject.meshNucleic Acid Hybridization
 
dc.subject.meshPolymorphism, Single Nucleotide
 
dc.subject.meshReproducibility of Results
 
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
 
dc.titleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University