Article: High-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers

File Download Links for fulltext
(May Require Subscription)
  • Basic View
  • Metadata View
  • XML View
TitleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
AuthorsTai, ALS
Mak, W1
Ng, PKM1
Chua, DTT
Ng, MYM1
Fu, L
Chu, KKW
Fang, Y2
You, QS1
Chen, M
Zhang, M
Sham, PC1
Guan, XY1 2
Issue Date2006
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
CitationCancer Research, 2006, v. 66 n. 8, p. 4133-4138 [How to Cite?]
DOI: http://dx.doi.org/10.1158/0008-5472.CAN-05-2775
AbstractLoss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.
ISSN0008-5472
2011 Impact Factor: 7.856
2011 SCImago Journal Rankings: 1.309
DOIhttp://dx.doi.org/10.1158/0008-5472.CAN-05-2775
ISI Accession Number IDWOS:000236843200025
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTai, ALS
dc.contributor.authorMak, W
dc.contributor.authorNg, PKM
dc.contributor.authorChua, DTT
dc.contributor.authorNg, MYM
dc.contributor.authorFu, L
dc.contributor.authorChu, KKW
dc.contributor.authorFang, Y
dc.contributor.authorYou, QS
dc.contributor.authorChen, M
dc.contributor.authorZhang, M
dc.contributor.authorSham, PC
dc.contributor.authorGuan, XY
dc.date.accessioned2010-09-06T09:05:15Z
dc.date.available2010-09-06T09:05:15Z
dc.date.issued2006
dc.description.abstractLoss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCancer Research, 2006, v. 66 n. 8, p. 4133-4138 [How to Cite?]
DOI: http://dx.doi.org/10.1158/0008-5472.CAN-05-2775
dc.identifier.doihttp://dx.doi.org/10.1158/0008-5472.CAN-05-2775
dc.identifier.epage4138
dc.identifier.hkuros115336
dc.identifier.hkuros128682
dc.identifier.isiWOS:000236843200025
dc.identifier.issn0008-5472
2011 Impact Factor: 7.856
2011 SCImago Journal Rankings: 1.309
dc.identifier.issue8
dc.identifier.openurl
dc.identifier.pmid16618734
dc.identifier.scopuseid_2-s2.0-33646241534
dc.identifier.spage4133
dc.identifier.urihttp://hdl.handle.net/10722/85466
dc.identifier.volume66
dc.languageeng
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
dc.publisher.placeUnited States
dc.relation.ispartofCancer Research
dc.relation.referencesReferences in Scopus
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics
dc.subject.meshChromosomes, Human, Pair 3 - genetics
dc.subject.meshGenes, Tumor Suppressor
dc.subject.meshGenotype
dc.subject.meshHumans
dc.subject.meshLoss of Heterozygosity
dc.subject.meshLung Neoplasms - genetics
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshPolymorphism, Single Nucleotide
dc.subject.meshReproducibility of Results
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
dc.titleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University