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Article: High-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers

TitleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers
Authors
Issue Date2006
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 2006, v. 66 n. 8, p. 4133-4138 How to Cite?
Abstract
Loss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/85466
ISSN
2013 Impact Factor: 9.284
2013 SCImago Journal Rankings: 5.627
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTai, ALSen_HK
dc.contributor.authorMak, Wen_HK
dc.contributor.authorNg, PKMen_HK
dc.contributor.authorChua, DTTen_HK
dc.contributor.authorNg, MYMen_HK
dc.contributor.authorFu, Len_HK
dc.contributor.authorChu, KKWen_HK
dc.contributor.authorFang, Yen_HK
dc.contributor.authorYou, QSen_HK
dc.contributor.authorChen, Men_HK
dc.contributor.authorZhang, Men_HK
dc.contributor.authorSham, PCen_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-09-06T09:05:15Z-
dc.date.available2010-09-06T09:05:15Z-
dc.date.issued2006en_HK
dc.identifier.citationCancer Research, 2006, v. 66 n. 8, p. 4133-4138en_HK
dc.identifier.issn0008-5472en_HK
dc.identifier.urihttp://hdl.handle.net/10722/85466-
dc.description.abstractLoss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is ∼180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer. ©2006 American Association for Cancer Research.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_HK
dc.relation.ispartofCancer Researchen_HK
dc.subject.meshCarcinoma, Non-Small-Cell Lung - geneticsen_HK
dc.subject.meshChromosomes, Human, Pair 3 - geneticsen_HK
dc.subject.meshGenes, Tumor Suppressoren_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLoss of Heterozygosityen_HK
dc.subject.meshLung Neoplasms - geneticsen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshPolymorphism, Single Nucleotideen_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_HK
dc.titleHigh-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-5472&volume=66&issue=8&spage=4133&epage=4138&date=2006&atitle=High-throughput+Loss-of-Heterozygosity+Study+of+Chromosome+3p+in+Lung+Cancer+Using+Single-Nucleotide+Polymorphism+Markersen_HK
dc.identifier.emailChua, DTT: dttchua@hkucc.hku.hken_HK
dc.identifier.emailFu, L: gracelfu@hku.hken_HK
dc.identifier.emailYou, QS: songy@hku.hken_HK
dc.identifier.emailSham, PC: pcsham@hku.hken_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.authorityChua, DTT=rp00415en_HK
dc.identifier.authorityFu, L=rp01435en_HK
dc.identifier.authorityYou, QS=rp00488en_HK
dc.identifier.authoritySham, PC=rp00459en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1158/0008-5472.CAN-05-2775en_HK
dc.identifier.pmid16618734en_HK
dc.identifier.scopuseid_2-s2.0-33646241534en_HK
dc.identifier.hkuros115336en_HK
dc.identifier.hkuros128682-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33646241534&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue8en_HK
dc.identifier.spage4133en_HK
dc.identifier.epage4138en_HK
dc.identifier.isiWOS:000236843200025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTai, ALS=8234187900en_HK
dc.identifier.scopusauthoridMak, W=22934897600en_HK
dc.identifier.scopusauthoridNg, PKM=13204578800en_HK
dc.identifier.scopusauthoridChua, DTT=7006773480en_HK
dc.identifier.scopusauthoridNg, MYM=8367886400en_HK
dc.identifier.scopusauthoridFu, L=22979236700en_HK
dc.identifier.scopusauthoridChu, KKW=22946688100en_HK
dc.identifier.scopusauthoridFang, Y=7403457405en_HK
dc.identifier.scopusauthoridYou, QS=7404921212en_HK
dc.identifier.scopusauthoridChen, M=13204327400en_HK
dc.identifier.scopusauthoridZhang, M=8852350800en_HK
dc.identifier.scopusauthoridSham, PC=34573429300en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK

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