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Article: Identification of a potential receptor for both peptide histidine isoleucine and peptide histidine valine

TitleIdentification of a potential receptor for both peptide histidine isoleucine and peptide histidine valine
Authors
Issue Date2002
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2002, v. 143 n. 4, p. 1327-1336 How to Cite?
AbstractPeptide histidine isoleucine (PHI), peptide histidine valine (PHV), and vasoactive intestinal polypeptide (VIP) are cosynthesized from the same precursor and share high levels of structural similarities with overlapping biological functions. In this study, the first PHI/PHV receptor was isolated and characterized in goldfish. To study this receptor using homologous peptides, we have also characterized the goldfish prepro-PHI/VIP, and, surprisingly, a shorter transcript lacking the VIP coding region was isolated. A PHI/VIP precursor without the VIP coding sequence has never before been reported. Initial functional expression of the PHI/PHV receptor in Chinese hamster ovary cells revealed that it could be activated by human PHV [50% effective concentration (EC50): 43 nM] and to a lesser extent human PHI (EC50: 133 nM) and helodermin (EC50: 166 nM) but not fish and mammalian pituitary adenylate cyclase-activating polypeptides and VIPs. Subsequent studies indicated that, similar to the pituitary adenylate cyclase-activating polypeptide receptors (PAC1-R, VPAC1-R, and VPAC2-R), the receptor isolated in this study is able to interact with goldfish PHI and its C-terminally extended form, PHV with EC50 values 93 and 43 nM, respectively. Northern blot and RT-PCP/Southern blot analyses revealed that the PHI/VIP gene is expressed in the intestine, brain, and gall bladder and the PHI/PHV receptor gene is primarily expressed in the pituitary and to a lesser extend in the intestine and gall bladder, suggesting that PHI/PHV may play a role, notably in the regulation of pituitary function. In conclusion, our results demonstrate for the first time the existence of a PHI/PHV receptor, indicating that the functions of PHI and PHV could be mediated by their own receptor in addition to VIP receptors.
Persistent Identifierhttp://hdl.handle.net/10722/84959
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDicky LaiYin, TSEen_HK
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorWong, AONLen_HK
dc.contributor.authorChan, SMen_HK
dc.contributor.authorVaudry, Hen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-09-06T08:59:06Z-
dc.date.available2010-09-06T08:59:06Z-
dc.date.issued2002en_HK
dc.identifier.citationEndocrinology, 2002, v. 143 n. 4, p. 1327-1336en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84959-
dc.description.abstractPeptide histidine isoleucine (PHI), peptide histidine valine (PHV), and vasoactive intestinal polypeptide (VIP) are cosynthesized from the same precursor and share high levels of structural similarities with overlapping biological functions. In this study, the first PHI/PHV receptor was isolated and characterized in goldfish. To study this receptor using homologous peptides, we have also characterized the goldfish prepro-PHI/VIP, and, surprisingly, a shorter transcript lacking the VIP coding region was isolated. A PHI/VIP precursor without the VIP coding sequence has never before been reported. Initial functional expression of the PHI/PHV receptor in Chinese hamster ovary cells revealed that it could be activated by human PHV [50% effective concentration (EC50): 43 nM] and to a lesser extent human PHI (EC50: 133 nM) and helodermin (EC50: 166 nM) but not fish and mammalian pituitary adenylate cyclase-activating polypeptides and VIPs. Subsequent studies indicated that, similar to the pituitary adenylate cyclase-activating polypeptide receptors (PAC1-R, VPAC1-R, and VPAC2-R), the receptor isolated in this study is able to interact with goldfish PHI and its C-terminally extended form, PHV with EC50 values 93 and 43 nM, respectively. Northern blot and RT-PCP/Southern blot analyses revealed that the PHI/VIP gene is expressed in the intestine, brain, and gall bladder and the PHI/PHV receptor gene is primarily expressed in the pituitary and to a lesser extend in the intestine and gall bladder, suggesting that PHI/PHV may play a role, notably in the regulation of pituitary function. In conclusion, our results demonstrate for the first time the existence of a PHI/PHV receptor, indicating that the functions of PHI and PHV could be mediated by their own receptor in addition to VIP receptors.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleIdentification of a potential receptor for both peptide histidine isoleucine and peptide histidine valineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=143&spage=1327&epage=1336&date=2002&atitle=Identification+of+a+Potential+Receptor+for+Both+Peptide+Histidine+Isoleucine+and+Peptide+Histidine+Valineen_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailWong, AONL: olwong@hkucc.hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityWong, AONL=rp00806en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.143.4.1327en_HK
dc.identifier.pmid11897689-
dc.identifier.scopuseid_2-s2.0-0036212655en_HK
dc.identifier.hkuros65864en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036212655&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume143en_HK
dc.identifier.issue4en_HK
dc.identifier.spage1327en_HK
dc.identifier.epage1336en_HK
dc.identifier.isiWOS:000174725000022-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDicky LaiYin, TSE=17340338500en_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridWong, AONL=7403147570en_HK
dc.identifier.scopusauthoridChan, SM=7404255669en_HK
dc.identifier.scopusauthoridVaudry, H=35446602600en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK

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