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Article: Molecular characterization of the Chinese isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the construction and characterization of the DNA vaccines

TitleMolecular characterization of the Chinese isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the construction and characterization of the DNA vaccines
Authors
Issue Date2005
PublisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.html
Citation
Journal of Animal and Veterinary Advances, 2005, v. 4 n. 1, p. 63-70 How to Cite?
Abstract
The genomic regions encoding ORFs 5 and 6 of the Chinese isolates named as AV strain of PRRSV found in Guangzhou are chosen and cloned into the mammalian expression vector pcDNA3.1(+) so as to construct the DNA vaccine. The objectives of this study are to first characterize this AV strain PRRSV emerged in Guangzhou, sequences based on ORF 5 and 6 were served as reference for further phylogenetic studies on the difference with North American (VR-2332) and the European (LV) strains. Investigation further focuses on the role of DNA vaccine constructed based on AV strain PRRSV in the generation of both humoral and lymphoproliferation immune responses and the possibility of using crude bacteria as adjuvant. Immunization with plasmid encoding ORF 5 or ORF 6 of AV strain PRRSV, with or without crude bacteria as adjuvant, induced anti-ORF 5 neutralizing antibodies or ORF 6 specific antibodies in both Balb/c mice and its natural host, pigs (trial experiment). Animals vaccinated with crude bacteria conjugated with ORF 5 encoded plasmid elicited stronger (which is around 80% more) and faster antibody responses than with plasmid construct alone as measured by ELISA. In addition, the increase in the number of blood lymphocytes obtained from vaccinated mice and pigs indicates the specificity of the lymphoproliferation responses to ORF 5-envelope protein & ORF 6 matrix protein. In summary, it demonstrates that ORF 5 & 6 plasmid construct with crude bacteria as adjuvant may be a potential candidate against this regional AV strain PRRSV isolated from Guangzhou, China.
Persistent Identifierhttp://hdl.handle.net/10722/84956
ISSN
2013 SCImago Journal Rankings: 0.202

 

DC FieldValueLanguage
dc.contributor.authorWong, EYLen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:59:04Z-
dc.date.available2010-09-06T08:59:04Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal of Animal and Veterinary Advances, 2005, v. 4 n. 1, p. 63-70en_HK
dc.identifier.issn1680-5593en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84956-
dc.description.abstractThe genomic regions encoding ORFs 5 and 6 of the Chinese isolates named as AV strain of PRRSV found in Guangzhou are chosen and cloned into the mammalian expression vector pcDNA3.1(+) so as to construct the DNA vaccine. The objectives of this study are to first characterize this AV strain PRRSV emerged in Guangzhou, sequences based on ORF 5 and 6 were served as reference for further phylogenetic studies on the difference with North American (VR-2332) and the European (LV) strains. Investigation further focuses on the role of DNA vaccine constructed based on AV strain PRRSV in the generation of both humoral and lymphoproliferation immune responses and the possibility of using crude bacteria as adjuvant. Immunization with plasmid encoding ORF 5 or ORF 6 of AV strain PRRSV, with or without crude bacteria as adjuvant, induced anti-ORF 5 neutralizing antibodies or ORF 6 specific antibodies in both Balb/c mice and its natural host, pigs (trial experiment). Animals vaccinated with crude bacteria conjugated with ORF 5 encoded plasmid elicited stronger (which is around 80% more) and faster antibody responses than with plasmid construct alone as measured by ELISA. In addition, the increase in the number of blood lymphocytes obtained from vaccinated mice and pigs indicates the specificity of the lymphoproliferation responses to ORF 5-envelope protein & ORF 6 matrix protein. In summary, it demonstrates that ORF 5 & 6 plasmid construct with crude bacteria as adjuvant may be a potential candidate against this regional AV strain PRRSV isolated from Guangzhou, China.-
dc.languageengen_HK
dc.publisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.htmlen_HK
dc.relation.ispartofJournal of Animal and Veterinary Advancesen_HK
dc.titleMolecular characterization of the Chinese isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the construction and characterization of the DNA vaccinesen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.hkuros101943en_HK
dc.identifier.volume4-
dc.identifier.issue1-
dc.identifier.spage63-
dc.identifier.epage70-
dc.publisher.placePakistan-

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