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Article: Correlation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development

TitleCorrelation of expression of preprothyrotropin-releasing hormone and receptor with rat testis development
Authors
KeywordsPrepro thyrotrophin-releasing hormone
Rat testis
Real time quantitative RT-PCR
Thyrotrophin-releasing hormone receptor
Issue Date2002
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2002, v. 115 n. 1, p. 12-16 How to Cite?
AbstractObjective. To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis. Methods. Oligonucleotide primers were designed from the sequences of rat hypothalamus prepro TRH (ppTRH) and pituitary TRH-R cDNA for reverse transcription polymerase chain reaction (RT-PCR). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Expression plasmids containing ppTRH and TRH-R genes were then constructed, and expression was found in E. coli DH5-α. ppTRH and TRH-R mRNA in the testis was quantitated in RNA samples prepared from rats at different development stages by real time quantitative RT-PCR. Results. The quantitative analyses demonstrated that no ppTRH and TRH mRNA could be detected at the earliest stage (day 8) . ppTRH and TRH mRNA signals were detected on day 15 and increased progressively on days 20, 35, 60 and 90. Conclusion. Our results suggest that rat testis could specifically express TRH and TRH-R, and the transcriptions of ppTRH and TRH-R genes in the rat testis were development-dependent. The acquirement of expressed products for ppTRH and TRH-R can be used for further research on the physiological significance of TRH and TRH-R expression in rat testis.
Persistent Identifierhttp://hdl.handle.net/10722/84948
ISSN
2015 Impact Factor: 0.957
2015 SCImago Journal Rankings: 0.428
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Zen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorLiu, Xen_HK
dc.contributor.authorXu, Ren_HK
dc.date.accessioned2010-09-06T08:58:59Z-
dc.date.available2010-09-06T08:58:59Z-
dc.date.issued2002en_HK
dc.identifier.citationChinese Medical Journal, 2002, v. 115 n. 1, p. 12-16en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84948-
dc.description.abstractObjective. To investigate the expression regulation of thyrotrophin-releasing hormone (TRH) and TRH receptor (TRH-R), and their role in the development of rat testis. Methods. Oligonucleotide primers were designed from the sequences of rat hypothalamus prepro TRH (ppTRH) and pituitary TRH-R cDNA for reverse transcription polymerase chain reaction (RT-PCR). Specific fragments of ppTRH and TRH-R cDNA were cloned and sequenced. Expression plasmids containing ppTRH and TRH-R genes were then constructed, and expression was found in E. coli DH5-α. ppTRH and TRH-R mRNA in the testis was quantitated in RNA samples prepared from rats at different development stages by real time quantitative RT-PCR. Results. The quantitative analyses demonstrated that no ppTRH and TRH mRNA could be detected at the earliest stage (day 8) . ppTRH and TRH mRNA signals were detected on day 15 and increased progressively on days 20, 35, 60 and 90. Conclusion. Our results suggest that rat testis could specifically express TRH and TRH-R, and the transcriptions of ppTRH and TRH-R genes in the rat testis were development-dependent. The acquirement of expressed products for ppTRH and TRH-R can be used for further research on the physiological significance of TRH and TRH-R expression in rat testis.en_HK
dc.languageengen_HK
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese Medical Journalen_HK
dc.subjectPrepro thyrotrophin-releasing hormoneen_HK
dc.subjectRat testisen_HK
dc.subjectReal time quantitative RT-PCRen_HK
dc.subjectThyrotrophin-releasing hormone receptoren_HK
dc.titleCorrelation of expression of preprothyrotropin-releasing hormone and receptor with rat testis developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0366-6999&volume=115&spage=12&epage=16&date=2001&atitle=Correlation+of+expression+of+preprothyrotropin-releasing+hormone+and+receptor+with+rat+testis+developmenten_HK
dc.identifier.emailXu, R: xuruojun@hkucc.hku.hken_HK
dc.identifier.authorityXu, R=rp00820en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid11930644-
dc.identifier.scopuseid_2-s2.0-0036159158en_HK
dc.identifier.hkuros66131en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036159158&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume115en_HK
dc.identifier.issue1en_HK
dc.identifier.spage12en_HK
dc.identifier.epage16en_HK
dc.identifier.isiWOS:000173671800003-
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridLi, Z=36066890400en_HK
dc.identifier.scopusauthoridZhang, Y=7601321843en_HK
dc.identifier.scopusauthoridLiu, X=8572202600en_HK
dc.identifier.scopusauthoridXu, R=7402813973en_HK

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