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Article: Expression of the carbohydrate recognition domain of bovine conglutinin and demonstration of its binding to iC3b and yeast mannan

TitleExpression of the carbohydrate recognition domain of bovine conglutinin and demonstration of its binding to iC3b and yeast mannan
Authors
Issue Date1996
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 1996, v. 218 n. 1, p. 260-266 How to Cite?
AbstractBovine conglutinin is a collagenous C-type lectin (collectin) that is found in bovine serum. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine conglutinin, has been overexpressed in E. coli. The recombinant protein has been successfully renatured and showed the same sugar binding specificity as the native protein and was able to bind to yeast mannan and complement-activated immune complexes. The binding was calcium-dependent and was inhibited by N-acetylglucosamine. The concentration of N-acetylglucosamine required for 50% inhibition of binding to mannan was 1.77 mM for recombinant conglutinin and 0.71 mM for native conglutinin, respectively. The recombinant conglutinin should be useful in the assay and purification of circulating immune complexes and for therapeutic purposes involving the removal of immune complexes from patient's plasma.
Persistent Identifierhttp://hdl.handle.net/10722/84932
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLim, BLen_HK
dc.contributor.authorHolmskov, Uen_HK
dc.date.accessioned2010-09-06T08:58:48Z-
dc.date.available2010-09-06T08:58:48Z-
dc.date.issued1996en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 1996, v. 218 n. 1, p. 260-266en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/84932-
dc.description.abstractBovine conglutinin is a collagenous C-type lectin (collectin) that is found in bovine serum. A recombinant protein, composed of the neck-region and the carbohydrate binding domain of bovine conglutinin, has been overexpressed in E. coli. The recombinant protein has been successfully renatured and showed the same sugar binding specificity as the native protein and was able to bind to yeast mannan and complement-activated immune complexes. The binding was calcium-dependent and was inhibited by N-acetylglucosamine. The concentration of N-acetylglucosamine required for 50% inhibition of binding to mannan was 1.77 mM for recombinant conglutinin and 0.71 mM for native conglutinin, respectively. The recombinant conglutinin should be useful in the assay and purification of circulating immune complexes and for therapeutic purposes involving the removal of immune complexes from patient's plasma.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.titleExpression of the carbohydrate recognition domain of bovine conglutinin and demonstration of its binding to iC3b and yeast mannanen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=218&spage=260&epage=266&date=1996&atitle=Expression+of+the+carbohydrate+recognition+domain+of+bovine+conglutinin+and+demonstration+of+its+binding+to+iC3b+and+yeast+mannanen_HK
dc.identifier.emailLim, BL: bllim@hkucc.hku.hken_HK
dc.identifier.authorityLim, BL=rp00744en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1006/bbrc.1996.0046en_HK
dc.identifier.pmid8573143-
dc.identifier.scopuseid_2-s2.0-0030024433en_HK
dc.identifier.hkuros21517en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030024433&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume218en_HK
dc.identifier.issue1en_HK
dc.identifier.spage260en_HK
dc.identifier.epage266en_HK
dc.identifier.isiWOS:A1996TP75400046-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLim, BL=7201983917en_HK
dc.identifier.scopusauthoridHolmskov, U=7004526416en_HK

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