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Article: Rat testicular myotubularin, a protein tyrosine phosphatase expressed by sertoli and germ cells, is a potential marker for studying cell-cell interactions in the rat testis

TitleRat testicular myotubularin, a protein tyrosine phosphatase expressed by sertoli and germ cells, is a potential marker for studying cell-cell interactions in the rat testis
Authors
Issue Date2000
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 2000, v. 185 n. 3, p. 366-385 How to Cite?
AbstractThe full-length cDNA encoding the entire open reading frame (ORF) of rat myotubularin (rMTM) was isolated from a rat testis expression library by PCR. Among the three ~2.9-kb cDNAs that were sequenced, one clone was different from the other two clones. It contained seven extra amino acids of FVVLNLQ; this short stretch of extra sequence was found between Gin421 and Phe422 within the SET (Suvar3-9, Enhancer-of-zeste, Trithorax) interacting domain (SID) of rMTM. The rMTM ORF had 1,713 bp encoding for a 571 amino acid polypeptide and a calculated molecular weight of 65.8 kDa. A comparison between its deduced amino acid sequence and the GenBank database using BLAST revealed a 53.1% identity with human myotubularin protein (hMTM1), which is a member of the protein tyrosine phosphatase (PTP) family associated with X-linked myotubular myopathy. A 22 amino acid peptide NH2-TKVNERYELCDTYPALLAVPAN was synthesized based on the deduced amino acid sequence of rMTM and used for antibody production. By using immunoblot analysis, a 66-kDa protein was indeed detected in both Sertoli and germ-cell cytosols. rMTM mRNA was found in various tissues but was predominantly expressed in the testis, ovary, and skeletal muscle. Sertoli cell rMTM expression was stimulated by germ cells and enhanced when inter-Sertoli junctions were being assembled in vitro. A drastic reduction in testicular rMTM steady-state mRNA level correlated with the depletion of germ cells from the testis in vivo following either glycerol or Ionidamine treatment. These results indicate that rMTM is a rat homologue of hMTM1 that may be a useful marker in monitoring the events of cell-cell interactions in the testis. (C) 2000 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/84926
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, JCHen_HK
dc.contributor.authorSamy, ETen_HK
dc.contributor.authorGrima, Jen_HK
dc.contributor.authorChung, SSWen_HK
dc.contributor.authorMruk, Den_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorSilvestrini, Ben_HK
dc.contributor.authorCheng, CYen_HK
dc.date.accessioned2010-09-06T08:58:44Z-
dc.date.available2010-09-06T08:58:44Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Cellular Physiology, 2000, v. 185 n. 3, p. 366-385en_HK
dc.identifier.issn0021-9541en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84926-
dc.description.abstractThe full-length cDNA encoding the entire open reading frame (ORF) of rat myotubularin (rMTM) was isolated from a rat testis expression library by PCR. Among the three ~2.9-kb cDNAs that were sequenced, one clone was different from the other two clones. It contained seven extra amino acids of FVVLNLQ; this short stretch of extra sequence was found between Gin421 and Phe422 within the SET (Suvar3-9, Enhancer-of-zeste, Trithorax) interacting domain (SID) of rMTM. The rMTM ORF had 1,713 bp encoding for a 571 amino acid polypeptide and a calculated molecular weight of 65.8 kDa. A comparison between its deduced amino acid sequence and the GenBank database using BLAST revealed a 53.1% identity with human myotubularin protein (hMTM1), which is a member of the protein tyrosine phosphatase (PTP) family associated with X-linked myotubular myopathy. A 22 amino acid peptide NH2-TKVNERYELCDTYPALLAVPAN was synthesized based on the deduced amino acid sequence of rMTM and used for antibody production. By using immunoblot analysis, a 66-kDa protein was indeed detected in both Sertoli and germ-cell cytosols. rMTM mRNA was found in various tissues but was predominantly expressed in the testis, ovary, and skeletal muscle. Sertoli cell rMTM expression was stimulated by germ cells and enhanced when inter-Sertoli junctions were being assembled in vitro. A drastic reduction in testicular rMTM steady-state mRNA level correlated with the depletion of germ cells from the testis in vivo following either glycerol or Ionidamine treatment. These results indicate that rMTM is a rat homologue of hMTM1 that may be a useful marker in monitoring the events of cell-cell interactions in the testis. (C) 2000 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_HK
dc.relation.ispartofJournal of Cellular Physiologyen_HK
dc.rightsJournal of Cellular Physiology. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleRat testicular myotubularin, a protein tyrosine phosphatase expressed by sertoli and germ cells, is a potential marker for studying cell-cell interactions in the rat testisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9541&volume=185&spage=366&epage=385&date=2000&atitle=Rat+Testicular+Myotubularin,+a+Protein+Tyrosine+Phosphatase+Expressed+by+Sertoli+and+Germ+Cells,+Is+a+Potential+Marker+for+Studying+Cell-Cell+Interactions+in+the+Rat+Testisen_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/1097-4652(200012)185:3<366::AID-JCP7>3.0.CO;2-1en_HK
dc.identifier.pmid11056007-
dc.identifier.scopuseid_2-s2.0-0033773233en_HK
dc.identifier.hkuros56827en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033773233&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume185en_HK
dc.identifier.issue3en_HK
dc.identifier.spage366en_HK
dc.identifier.epage385en_HK
dc.identifier.isiWOS:000165129400007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, JCH=37039127300en_HK
dc.identifier.scopusauthoridSamy, ET=6602891563en_HK
dc.identifier.scopusauthoridGrima, J=7003383792en_HK
dc.identifier.scopusauthoridChung, SSW=35104555300en_HK
dc.identifier.scopusauthoridMruk, D=6701823934en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridSilvestrini, B=7006825900en_HK
dc.identifier.scopusauthoridCheng, CY=7404797787en_HK

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