File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification and regulation of receptor tyrosine kinases Rse and Mer and their ligand Gas6 in testicular somatic cells

TitleIdentification and regulation of receptor tyrosine kinases Rse and Mer and their ligand Gas6 in testicular somatic cells
Authors
KeywordsSertoli cells
Testicular cell lines
Tyrosine phosphorylation
Issue Date2000
PublisherAmerican Society of Andrology. The Journal's web site is located at http://www.andrologyjournal.org
Citation
Journal Of Andrology, 2000, v. 21 n. 2, p. 291-302 How to Cite?
AbstractReceptor tyrosine kinases act to convey extracellular signals to intracellular signaling pathways and ultimately control cell proliferation and differentiation. Rse, Axl, and Mer belong to a newly identified family of cell adhesion molecule-related receptor tyrosine kinase. They bind the vitamin K-dependent protein growth arrest-specific gene 6 (Gas6), which is also structurally related to the anticoagulation factor Protein S. The aim of this study is to investigate the possible role of Rse/Axl/Mer tyrosine kinase receptors and their ligand in regulating testicular functions. Gene expression of Rse, Axl, Mer, and Gas6 in the testis was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blot analysis. The results indicated that receptors Rse and Mer and the ligand Gas6 were expressed in the rat endothelial cell line (TR1), mouse Leydig cell line (TM3), rat peritubular myoid cell line (TRM), mouse Sertoli cell line (TM4), and primary rat Sertoli cells. Axl was not expressed in the testicular somatic cells by RT-PCR or Northern blot analysis. The highest level of expression of Gas6 messenger RNA (mRNA) was observed in the Sertoli cells, and its expression was responsive to the addition of forskolin in vitro. The effects of serum, insulin, and transferrin on Gas6 expression by TM4 cells were examined. It was shown that they all exhibited an up-regulating effect on Gas6 expression. The forskolin-stimulated Gas6 expression was accompanied by an increase in tyrosine phosphorylation of the Rse receptor in vitro, suggesting that Gas6 may exhibit an autocrine effect in the Sertoli cells through multiple tyrosine kinase receptors. Our studies so far have demonstrated that tyrosine kinase receptors Rse and Mer and their ligand Gas6 are widely expressed in the testicular somatic cell lines and may play a marked role in promoting testicular cell survival.
Persistent Identifierhttp://hdl.handle.net/10722/84924
ISSN
2014 Impact Factor: 2.473
2015 SCImago Journal Rankings: 0.867
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, MCWen_HK
dc.contributor.authorMather, JPen_HK
dc.contributor.authorMcCray, Gen_HK
dc.contributor.authorLee, WMen_HK
dc.date.accessioned2010-09-06T08:58:42Z-
dc.date.available2010-09-06T08:58:42Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Andrology, 2000, v. 21 n. 2, p. 291-302en_HK
dc.identifier.issn0196-3635en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84924-
dc.description.abstractReceptor tyrosine kinases act to convey extracellular signals to intracellular signaling pathways and ultimately control cell proliferation and differentiation. Rse, Axl, and Mer belong to a newly identified family of cell adhesion molecule-related receptor tyrosine kinase. They bind the vitamin K-dependent protein growth arrest-specific gene 6 (Gas6), which is also structurally related to the anticoagulation factor Protein S. The aim of this study is to investigate the possible role of Rse/Axl/Mer tyrosine kinase receptors and their ligand in regulating testicular functions. Gene expression of Rse, Axl, Mer, and Gas6 in the testis was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blot analysis. The results indicated that receptors Rse and Mer and the ligand Gas6 were expressed in the rat endothelial cell line (TR1), mouse Leydig cell line (TM3), rat peritubular myoid cell line (TRM), mouse Sertoli cell line (TM4), and primary rat Sertoli cells. Axl was not expressed in the testicular somatic cells by RT-PCR or Northern blot analysis. The highest level of expression of Gas6 messenger RNA (mRNA) was observed in the Sertoli cells, and its expression was responsive to the addition of forskolin in vitro. The effects of serum, insulin, and transferrin on Gas6 expression by TM4 cells were examined. It was shown that they all exhibited an up-regulating effect on Gas6 expression. The forskolin-stimulated Gas6 expression was accompanied by an increase in tyrosine phosphorylation of the Rse receptor in vitro, suggesting that Gas6 may exhibit an autocrine effect in the Sertoli cells through multiple tyrosine kinase receptors. Our studies so far have demonstrated that tyrosine kinase receptors Rse and Mer and their ligand Gas6 are widely expressed in the testicular somatic cell lines and may play a marked role in promoting testicular cell survival.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Andrology. The Journal's web site is located at http://www.andrologyjournal.orgen_HK
dc.relation.ispartofJournal of Andrologyen_HK
dc.subjectSertoli cellsen_HK
dc.subjectTesticular cell linesen_HK
dc.subjectTyrosine phosphorylationen_HK
dc.subject.meshIntercellular Signaling Peptides and Proteins-
dc.subject.meshNeural Cell Adhesion Molecules - metabolism-
dc.subject.meshProteins - genetics - metabolism-
dc.subject.meshReceptor Protein-Tyrosine Kinases - genetics - metabolism-
dc.subject.meshTestis - cytology - enzymology - metabolism-
dc.titleIdentification and regulation of receptor tyrosine kinases Rse and Mer and their ligand Gas6 in testicular somatic cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, MCW: mchan@hku.hken_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityChan, MCW=rp00420en_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10714824-
dc.identifier.scopuseid_2-s2.0-0033994339en_HK
dc.identifier.hkuros48609en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033994339&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue2en_HK
dc.identifier.spage291en_HK
dc.identifier.epage302en_HK
dc.identifier.isiWOS:000085540900015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, MCW=26654715500en_HK
dc.identifier.scopusauthoridMather, JP=35512944300en_HK
dc.identifier.scopusauthoridMcCray, G=6701506586en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats